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PMID: 2984674 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

cDNA-derived amino acid sequence of the gamma subunit of GTPase from bovine rod outer segments.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 82 ·No. 7 ·1985-04-00 ·Pages 1936-40

Yatsunami K, Pandya BV, Oprian DD, Khorana HG

Abstract

The sequence of the 74 amino acids in the gamma subunit of GTPase from bovine rod outer segments has been deduced from the cDNA sequence. Enriched GTPase mRNA was used to prepare a cDNA library in the expression vector lambda gt11. Clones encoding the gamma subunit were identified by screening the library with anti-GTPase antibodies and by cell-free translation of hybrid-selected mRNA. The longest cDNA (448 base pairs) contained the amino acid coding region for the entire gamma subunit as well as 5' and 3' noncoding regions of 67 and 159 base pairs, respectively. The 3' noncoding region contained the sequence, A-A-U-A-A-A, the presumed recognition sequence for polyadenylylation, and a 14-unit long poly(A) tract. The amino acid sequence derived for the gamma-subunit is mostly in agreement with that previously determined by McConnell and co-workers [McConnell, D. G., Kohnken, R. E. & Smith, A. J. (1984) Fed. Proc. Fed. Am. Soc. Exp. Biol. 43, 1585] and the partial sequence deduced by Van Dop and co-workers from cDNA [Van Dop, C., Medynski, D., Sullivan, K., Wu, A. M., Fung, B. K.-K. & Bourne, H. R. (1984) Biochem. Biophys. Res. Commun. 124, 250-255].

MeSH Terms
Amino Acid Sequence Animals Base Sequence Cattle Cloning, Molecular DNA/analysis GTP Phosphohydrolases/analysis,genetics Phosphoric Monoester Hydrolases/analysis Photoreceptor Cells/enzymology RNA, Messenger/metabolism Rod Cell Outer Segment/enzymology
Chemicals
RNA, Messenger DNA Phosphoric Monoester Hydrolases GTP Phosphohydrolases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Yatsunami K
Pandya B V
Oprian D D
Khorana H G
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37 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.82.7.1936
Published
1985-04-00
Pages
1936-40
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC397451
Subset
IM
Grants
NIAID NIH HHS · AI11479 · United States
NIGMS NIH HHS · GM28289 · United States
Databases
GENBANK
K03255
External Links
PubMed source DOI: 10.1073/pnas.82.7.1936 PMC Full Text View journal details
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