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PMID: 2824831 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Map location of the thymidine kinase gene of bovine herpesvirus 1.

Journal of virology ·Vol. 61 ·No. 12 ·1987-12-00 ·Pages 4023-5

Bello LJ, Whitbeck JC, Lawrence WC

Abstract

Bovine herpesvirus 1 has been reported to contain a thymidine kinase (tk) gene which is nonessential for virus replication. We have isolated a thymidine kinase-negative mutant of the virus and localized the mutation by marker rescue experiments to a 1.1-kilobase BglII-SalI fragment which maps at 0.47 to 0.48 on the bovine herpesvirus 1 genomic map. A thymidine kinase-negative bovine cell line isolated in our laboratory was used in these studies.

MeSH Terms

Animals Cattle Cell Line Cloning, Molecular DNA Restriction Enzymes DNA, Viral/genetics Deoxyribonuclease HindIII Genes, Viral Genetic Markers Herpesvirus 1, Bovine/enzymology,genetics Mutation Phenotype Thymidine Kinase/genetics

Chemicals

DNA, Viral Genetic Markers Thymidine Kinase DNA Restriction Enzymes Deoxyribonuclease HindIII

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Bello L J

Laboratory of Microbiology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia 19104-6049.

Whitbeck J C

Lawrence W C

References (14)

14 references, click to expand

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Article Info

Journal

Journal of virology

Abbr.

J Virol

ISSN

0022-538X

DOI

10.1128/JVI.61.12.4023-4025.1987

Published

1987-12-00

Pages

4023-5

Language

English

Region

United States

NLM ID

0113724

PMCID

PMC256025

Subset

External Links

PubMed source DOI: 10.1128/JVI.61.12.4023-4025.1987 PMC Full Text View journal details

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Map location of the thymidine kinase gene of bovine herpesvirus 1.

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