Abstract
A line of 3T3 mouse cells lacking deoxythymidine kinase (dTK-) was stably transformed to the dTK+ phenotype after exposure to UV-irradiated equine herpesvirus type 1 (EHV-1). Biochemical transformants were isolated in a system selective for the dTK+ phenotype (Eagle minimal essential medium containing 10(-4) M hypoxanthine, 6 X 10(-7) M aminopterin, and 2 X 10(-5) M deoxythymidine). Transformation was accompanied by the acquisition of a dTK activity with immunological, electrophoretic, and biochemical characteristics identical to those of the dTK induced by EHV-1 during productive infection. The transformed cells have been maintained in selective culture medium for more than 50 passages and have retained the capacity to express EHV-1--specific antigens. Spontaneous release of infectious virus has not been detected in the transformed lines, and the the cells were not oncogenic for athymic nude mice. In contrast to normal dTk+ 3T3 cells, EHV-1 transformants were unable to grow in the presence of arabinosylthymine, a drug selectively phosphorylated by herpesvirus-coded dTK's. These results indicate that a portion of the EHV-1 genome is able to persist in the transformed cells for many generations and be expressed as an enzymatically active viral gene product.
MeSH Terms
Animals
Antigens, Viral/analysis
Arabinonucleosides/pharmacology
Cell Division/drug effects
Cell Line
Cell Transformation, Viral/radiation effects
Herpesviridae/growth & development
Herpesvirus 1, Equid/growth & development,immunology,radiation effects
Mice
Mice, Nude
Neoplasms, Experimental/etiology
Phenotype
Thymidine/analogs & derivatives,pharmacology
Thymidine Kinase/genetics,metabolism
Ultraviolet Rays
Chemicals
Antigens, Viral
Arabinonucleosides
Thymidine Kinase
thymine arabinoside
Thymidine
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Allen G P
McGowan J J
Gentry G A
Randall C C
References (27)
27 references, click to expand
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