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PMID: 2555700 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Properties of the DNA-binding domain of the simian virus 40 large T antigen.

Molecular and cellular biology ·Vol. 9 ·No. 12 ·1989-12-00 ·Pages 5525-36

McVey D, Strauss M, Gluzman Y

Abstract

T antigen (Tag) from simian virus 40 binds specifically to two distinct sites in the viral origin of replication and to single-stranded DNA. Analysis of the protein domain responsible for these activities revealed the following. (i) The C-terminal boundary of the origin-specific and single-strand-specific DNA-binding domain is at or near amino acid 246; furthermore, the maximum of these DNA-binding activities coincides with a narrow C-terminal boundary, spanning 4 amino acids (246 to 249) and declines sharply in proteins with C termini which differ by a few (4 to 10) amino acids; (ii) a polypeptide spanning residues 132 to 246 of Tag is an independent domain responsible for origin-specific DNA binding and presumably for single-stranded DNA binding; and (iii) a comparison of identical N-terminal fragments of Tag purified from mammalian and bacterial cells revealed differential specificity and levels of activity between the two sources of protein. A role for posttranslational modification (phosphorylation) in controlling the DNA-binding activity of Tag is discussed.

MeSH Terms
Amino Acid Sequence Antigens, Polyomavirus Transforming Base Sequence Chromosome Deletion DNA Replication DNA, Single-Stranded/genetics,metabolism DNA, Viral/genetics,metabolism DNA-Binding Proteins/metabolism Genes, Fungal HeLa Cells/metabolism Humans Molecular Sequence Data Nucleic Acid Heteroduplexes/genetics,metabolism Oligonucleotide Probes/chemical synthesis Plasmids Recombination, Genetic Simian virus 40/genetics,immunology Templates, Genetic
Chemicals
Antigens, Polyomavirus Transforming DNA, Single-Stranded DNA, Viral DNA-Binding Proteins Nucleic Acid Heteroduplexes Oligonucleotide Probes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
McVey D
Cold Spring Harbor Laboratory, New York 11724.
Strauss M
Gluzman Y
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72 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.9.12.5525-5536.1989
Published
1989-12-00
Pages
5525-36
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC363723
Subset
IM
Grants
NCI NIH HHS · CA 13106 · United States
External Links
PubMed source DOI: 10.1128/mcb.9.12.5525-5536.1989 PMC Full Text View journal details
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