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PMID: 3041053 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

Studies on the origin-specific DNA-binding domain of simian virus 40 large T antigen.

Journal of virology ·Vol. 61 ·No. 10 ·1987-10-00 ·Pages 3326-30

Strauss M, Argani P, Mohr IJ, Gluzman Y

Abstract

The origin-specific DNA-binding domain of simian virus 40 large T antigen was analyzed, and its C-terminal boundary was found to be at or before amino acid 259. This does not include the zinc finger structural motif located at amino acids 302 to 320 (J. M. Berg, Science 232:485-486, 1986). Interestingly, N-terminal fragments of 266 and 272 amino acids and larger displayed dramatically reduced origin-binding activity. In addition, the specific DNA-binding properties of truncated proteins purified from both bacterial and mammalian sources were compared. Truncated T antigens from mammalian cells bound specific DNA fragments more efficiently than did their bacterial counterparts. These results implicate posttranslational modification with a role in regulating the DNA-binding activity of large T antigen.

MeSH Terms
Animals Antigens, Polyomavirus Transforming Antigens, Viral, Tumor/analysis,genetics,metabolism Bacterial Proteins/metabolism DNA, Viral/metabolism DNA-Binding Proteins/analysis HeLa Cells Humans Oncogene Proteins, Viral/analysis,genetics,metabolism Protein Processing, Post-Translational Simian virus 40/genetics,immunology
Chemicals
Antigens, Polyomavirus Transforming Antigens, Viral, Tumor Bacterial Proteins DNA, Viral DNA-Binding Proteins Oncogene Proteins, Viral
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Strauss M
Argani P
Mohr I J
Gluzman Y
References (33)
33 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.61.10.3326-3330.1987
Published
1987-10-00
Pages
3326-30
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC255919
Subset
IM
Grants
NCI NIH HHS · CA 13106 · United States
External Links
PubMed source DOI: 10.1128/JVI.61.10.3326-3330.1987 PMC Full Text View journal details
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