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PMID: 3367427 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Simian virus 40 (SV40) T antigen binds specifically to double-stranded DNA but not to single-stranded DNA or DNA/RNA hybrids containing the SV40 regulatory sequences.

Journal of virology ·Vol. 62 ·No. 6 ·1988-06-00 ·Pages 2204-8

Auborn KJ, Markowitz RB, Wang E, Yu YT, Prives C

Abstract

Simian virus 40 T antigen has been shown previously to bind specifically with high affinity to sites within the regulatory region of double-stranded simian virus 40 DNA. Using competition filter binding and the DNA-binding immunoassay, we show that T antigen did not bind specifically to either early or late single-stranded DNA containing these binding sites. Moreover, T antigen did not bind these sequences present in single-stranded RNA, RNA/RNA duplexes, or RNA/DNA hybrids. T antigen did, however, bind as efficiently to single-stranded DNA-cellulose as to double-stranded DNA-cellulose. This binding was nonspecific because it was independent of the presence of T-antigen-binding sites. The implications of these observations are discussed.

MeSH Terms
Antigens, Polyomavirus Transforming/metabolism Binding, Competitive DNA, Single-Stranded/metabolism DNA, Viral/metabolism DNA-Binding Proteins/metabolism Nucleic Acid Hybridization RNA, Viral/metabolism Regulatory Sequences, Nucleic Acid Transcription Factors/metabolism
Chemicals
Antigens, Polyomavirus Transforming DNA, Single-Stranded DNA, Viral DNA-Binding Proteins RNA, Viral Transcription Factors
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Auborn K J
Department of Biological Sciences, Columbia University, New York, New York 10027.
Markowitz R B
Wang E
Yu Y T
Prives C
References (44)
44 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.62.6.2204-2208.1988
Published
1988-06-00
Pages
2204-8
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC253332
Subset
IM
Grants
NCI NIH HHS · CA07536 · United States
NCI NIH HHS · CA07571 · United States
NCI NIH HHS · CA26905 · United States
External Links
PubMed source DOI: 10.1128/JVI.62.6.2204-2208.1988 PMC Full Text View journal details
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