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PMID: 2439897 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Alterations in pp60c-src accompany differentiation of neurons from rat embryo striatum.

Molecular and cellular biology ·Vol. 7 ·No. 5 ·1987-05-00 ·Pages 1830-40

Cartwright CA, Simantov R, Kaplan PL, Hunter T, Eckhart W

Abstract

Cultured neurons from rat embryo striatum were found to contain two structurally distinct forms of pp60c-src. The 60-kilodalton (kDa) form appeared similar to pp60c-src from cultured rat fibroblasts or astrocytes. The 61-kDa form was specific to neurons and differed in the NH2-terminal 18 kDa of the molecule. In undifferentiated neurons the predominant phosphorylated species of pp60c-src was the fibroblast form. Upon differentiation, a second phosphorylated form of pp60c-src was detected. This form had two or more additional sites of serine phosphorylation within the NH2-terminal 18-kDa region of the molecule, one of which was Ser-12. The specific protein-tyrosine kinase activity of the total pp60c-src population increased 14-fold, as measured by autophosphorylation, or 7-fold, as measured by phosphorylation of an exogenous substrate, as striatal neurons differentiated. This elevation in protein kinase activity occurred without a detectable decrease in Tyr-527 phosphorylation or increase in Tyr-416 phosphorylation. Our results support the idea that the expression of the neuron-specific form of pp60c-src and the increase in specific protein kinase activity may be important for neuronal differentiation.

MeSH Terms
Animals Cell Differentiation Cells, Cultured Corpus Striatum/physiology Culture Media Enzyme Activation Gene Expression Regulation Neurons/physiology Phosphoproteins/genetics Phosphoserine/metabolism Protein-Tyrosine Kinases/genetics Proto-Oncogene Proteins/genetics Proto-Oncogene Proteins pp60(c-src) Rats
Chemicals
Culture Media Phosphoproteins Proto-Oncogene Proteins Phosphoserine Protein-Tyrosine Kinases Proto-Oncogene Proteins pp60(c-src)
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Cartwright C A
Simantov R
Kaplan P L
Hunter T
Eckhart W
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.7.5.1830-1840.1987
Published
1987-05-00
Pages
1830-40
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC365286
Subset
IM
Grants
NCI NIH HHS · CA-01040 · United States
NCI NIH HHS · CA-13884 · United States
NCI NIH HHS · CA-14195 · United States
External Links
PubMed source DOI: 10.1128/mcb.7.5.1830-1840.1987 PMC Full Text View journal details
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