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PMID: 1900917 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

DNase I-hypersensitive sites and transcription factor-binding motifs within the mouse E beta meiotic recombination hot spot.

Molecular and cellular biology ·Vol. 11 ·No. 4 ·1991-04-00 ·Pages 1813-9

Shenkar R, Shen MH, Arnheim N

Abstract

The second intron of the E beta gene in the mouse major histocompatibility complex is the site of a meiotic recombination hot spot. We detected two DNase I-hypersensitive sites in this intron in meiotic cells isolated from mouse testes. One site appears to be constitutive and is found in other tissues regardless of whether or not they express the E beta gene. Near this hypersensitive site are potential binding motifs for H2TF1/KBF1, NF kappa B, and octamer transcription factors. Gel retardation studies with mouse lymphoma cell nuclear extracts confirmed that each of these motifs is capable of binding protein. The binding of transcription factors may contribute to the enhancement of recombination potential by altering chromatin structure and increasing the accessibility of the DNA to the recombination machinery.

Related Genes
E&Bgr
MeSH Terms
Animals Base Sequence Binding Sites Cell Line Deoxyribonuclease I/metabolism Female Genes Genes, MHC Class II Introns Male Meiosis Methylation Mice Molecular Sequence Data Recombination, Genetic Restriction Mapping Testis/cytology,metabolism Transcription Factors/metabolism
Chemicals
Transcription Factors Deoxyribonuclease I
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Shenkar R
Molecular Biology Section, University of Southern California, Los Angeles 90089-1340.
Shen M H
Arnheim N
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.11.4.1813-1819.1991
Published
1991-04-00
Pages
1813-9
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC359851
Subset
IM
Grants
NIA NIH HHS · AG00093 · United States
NIGMS NIH HHS · GM36745 · United States
External Links
PubMed source DOI: 10.1128/mcb.11.4.1813-1819.1991 PMC Full Text View journal details
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