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PMID: 1986228 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A poly(dA.dT) tract is a component of the recombination initiation site at the ARG4 locus in Saccharomyces cerevisiae.

Molecular and cellular biology ·Vol. 11 ·No. 1 ·1991-01-00 ·Pages 322-8

Schultes NP, Szostak JW

Abstract

An initiation site for meiotic gene conversion is located in the promoter region of the ARG4 locus in Saccharomyces cerevisiae. We have tested the hypothesis that the initiation site is identical with the promoter by making a series of small deletions that remove specific promoter elements. Disruption of most promoter elements does not lower the level of gene conversion in ARG4, and analysis of RNA levels at the time of recombination in meiosis reveals no direct correlation between the level of ARG4 transcript and the level of gene conversion in ARG4. However, deletion of a tract of 14 A residues located at the peak of the gene conversion gradient decreases the number of gene conversion events stimulated by the initiation site to 25 to 35% of the normal level. We conclude that the poly(dA.dT) tract is responsible for most but not all of the high levels of meiotic gene conversion observed in ARG4.

Related Genes
ARG4
MeSH Terms
Cloning, Molecular DNA Mutational Analysis DNA, Fungal/genetics Gene Conversion Meiosis Poly dA-dT/genetics Promoter Regions, Genetic Recombination, Genetic Saccharomyces cerevisiae/genetics Transcription, Genetic
Chemicals
DNA, Fungal Poly dA-dT
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Schultes N P
Department of Molecular Biology, Massachusetts General Hospital, Boston 02114.
Szostak J W
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30 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.11.1.322-328.1991
Published
1991-01-00
Pages
322-8
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC359622
Subset
IM
External Links
PubMed source DOI: 10.1128/mcb.11.1.322-328.1991 PMC Full Text View journal details
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