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PMID: 3600654 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Chromatin structure of the HLA-DR alpha gene in different functional states of major histocompatibility complex class II gene expression.

Molecular and cellular biology ·Vol. 7 ·No. 5 ·1987-05-00 ·Pages 1967-72

Peterlin BM, Hardy KJ, Larsen AS

Abstract

We utilized DNase I hypersensitivity mapping to study chromatin structure within the HLA-DR alpha gene. We found a single DNase I-hypersensitive site coinciding with the HLA-DR alpha gene promoter in all cells studied. Moreover, in cells that constitutively express HLA-DR, two additional DNase I-hypersensitive sites were observed. These lie within the first intron of the HLA-DR alpha gene and encompass DNA sequences that share homologies with regulatory loci of the immunoglobulin and immune response genes, as well as with core enhancer consensus sequences.

MeSH Terms
Base Sequence Cell Line Chromatin/ultrastructure Chromosome Mapping Deoxyribonuclease I Gene Expression Regulation Genes HLA-D Antigens/genetics HLA-DR Antigens/genetics Humans Transcription, Genetic
Chemicals
Chromatin HLA-D Antigens HLA-DR Antigens Deoxyribonuclease I
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Peterlin B M
Hardy K J
Larsen A S
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29 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.7.5.1967-1972.1987
Published
1987-05-00
Pages
1967-72
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC365303
Subset
IM
Grants
NIADDK NIH HHS · AM33004 · United States
External Links
PubMed source DOI: 10.1128/mcb.7.5.1967-1972.1987 PMC Full Text View journal details
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