Home LiteratureArticle Details
PMID: 2987832 Published · ppublish English Journal Article

Complete nucleotide sequence of a functional HLA-DP beta gene and the region between the DP beta 1 and DP alpha 1 genes: comparison of the 5' ends of HLA class II genes.

Nucleic acids research ·Vol. 13 ·No. 5 ·1985-03-11 ·Pages 1607-21

Kelly A, Trowsdale J

Abstract

The complete nucleotide sequence of an HLA-DP beta 1 gene and part of the adjacent DP alpha 1 gene, up to and including the signal sequence exon, were determined. The sequence of the DP beta 1 gene identified it as the DPw4 allele. The six exons of the DP beta 1 gene spanned over 11,000 bp of sequence. The arrangement of the gene was broadly analogous to genes of other class II beta chains. The beta 1 exon was flanked by introns of over 4 kb. Comparisons with published sequences of cDNA clones indicated that an alternative splice junction, at the 3' end of the gene, is used in at least one allele. Variation in choice of splice junction indicates an additional mechanism for allelic variation in class II genes. The sequence also indicated that the DP beta 1 and DP alpha 1 genes are separated by only 2 kb at their 5' ends. Comparison of the 5' ends of the DP alpha 1 and beta 1 genes with other class II sequences, including the DZ alpha gene, showed conservation of several blocks of sequences thought to be involved in control of expression. Some areas of the introns were partially conserved in the DQ beta gene, and several other intron sequences were homologous to sequences found in other unrelated genes.

MeSH Terms
Alleles Amino Acid Sequence Base Sequence DNA Restriction Enzymes/metabolism HLA-DP Antigens Histocompatibility Antigens Class II/genetics
Chemicals
HLA-DP Antigens Histocompatibility Antigens Class II DNA Restriction Enzymes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Kelly A
Trowsdale J
References (43)
43 references, click to expand
  1. The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers.
    Gene. 1982 Oct;19(3):259-68 PMID: 6295879
  2. Automation of the computer handling of gel reading data produced by the shotgun method of DNA sequencing.
    Nucleic Acids Res. 1982 Aug 11;10(15):4731-51 PMID: 7133997
  3. Independently evolving chicken histone H2B genes: identification of a ubiquitous H2B-specific 5' element.
    Nucleic Acids Res. 1982 Dec 11;10(23):7851-63 PMID: 6296794
  4. Rapid similarity searches of nucleic acid and protein data banks.
    Proc Natl Acad Sci U S A. 1983 Feb;80(3):726-30 PMID: 6572363
  5. The murine E alpha immune response gene.
    Cell. 1983 Mar;32(3):745-54 PMID: 6403249
  6. Association of two different repetitive DNA elements near immunoglobulin light chain genes.
    Nucleic Acids Res. 1983 Mar 25;11(6):1803-17 PMID: 6300779
  7. Sequences related to HLA-DR alpha chain on human chromosome 6: restriction enzyme polymorphism detected with DC alpha chain probes.
    Proc Natl Acad Sci U S A. 1983 Apr;80(7):1972-6 PMID: 6300884
  8. Structure and nucleotide sequence of the heavy chain gene of HLA-DR.
    Proc Natl Acad Sci U S A. 1983 Jun;80(12):3543-7 PMID: 6304715
  9. Specific association of repetitive DNA sequences with major histocompatibility genes.
    Mol Cell Biol. 1983 May;3(5):903-13 PMID: 6191198
  10. The complete nucleotide sequence of the I-E alpha d immune response gene.
    Nucleic Acids Res. 1983 Aug 11;11(15):5055-71 PMID: 6308570
  11. Nucleotide sequence of a light chain gene of the mouse I-A subregion: A beta d.
    Science. 1983 Aug 19;221(4612):750-4 PMID: 6410508
  12. Complete primary structures of the E beta chain and gene of the mouse major histocompatibility complex.
    Proc Natl Acad Sci U S A. 1983 Sep;80(18):5520-4 PMID: 6310581
  13. Genetic mapping of a human class II antigen beta-chain cDNA clone to the SB region of the HLA complex.
    Proc Natl Acad Sci U S A. 1983 Oct;80(19):6036-40 PMID: 6310612
  14. Molecular analysis of human class II transplantation antigens and their genes.
    Hum Immunol. 1983 Sep;8(1):95-103 PMID: 6415003
  15. The heavy chain of human B-cell alloantigen HLA-DS has a variable N-terminal region and a constant immunoglobulin-like region.
    Nature. 1983 Oct 27-Nov 2;305(5937):813-5 PMID: 6415485
  16. Intergenic DNA sequences flanking the pseudo alpha globin genes of human and chimpanzee.
    Nucleic Acids Res. 1983 Nov 25;11(22):8087-101 PMID: 6316284
  17. Both alpha and beta chains of HLA-DC class II histocompatibility antigens display extensive polymorphism in their amino-terminal domains.
    EMBO J. 1984 Feb;3(2):447-52 PMID: 6585297
  18. Isolation of a cDNA clone coding for an SB beta-chain.
    Nature. 1984 May 3-9;309(5963):76-8 PMID: 6325928
  19. Structure of the 5' ends of immunoglobulin genes: a novel conserved sequence.
    Proc Natl Acad Sci U S A. 1984 May;81(9):2650-4 PMID: 6425835
  20. Six HLA-D region alpha-chain genes on human chromosome 6: polymorphisms and associations of DC alpha-related sequences with DR types.
    Proc Natl Acad Sci U S A. 1984 Jun;81(11):3461-5 PMID: 6328517
  21. Correct transcription of an immunoglobulin kappa gene requires an upstream fragment containing conserved sequence elements.
    Nature. 1984 Jul 5-11;310(5972):71-4 PMID: 6330567
  22. Cell type-specific enhancer element associated with a mouse MHC gene, E beta.
    Nature. 1984 Aug 16-22;310(5978):594-7 PMID: 6087163
  23. Linkage map of two HLA-SB beta and two HLA-SB alpha-related genes: an intron in one of the SB beta genes contains a processed pseudogene.
    Cell. 1984 Aug;38(1):241-9 PMID: 6088068
  24. Cloning and sequence analysis of the human major histocompatibility complex gene DC-3 beta.
    Proc Natl Acad Sci U S A. 1984 Aug;81(16):5199-203 PMID: 6206493
  25. Homozygous deletions that simultaneously eliminate expressions of class I and class II antigens of EBV-transformed B-lymphoblastoid cells. I. Reduced proliferative responses of autologous and allogeneic T cells to mutant cells that have decreased expression of class II antigens.
    Hum Immunol. 1984 Oct;11(2):77-97 PMID: 6092300
  26. Functional insertion of an Alu type 2 (B2 SINE) repetitive sequence in murine class I genes.
    Science. 1984 Nov 23;226(4677):974-7 PMID: 6095445
  27. Complete nucleotide sequence of the E. coli glutathione synthetase gsh-II.
    Nucleic Acids Res. 1984 Dec 21;12(24):9299-307 PMID: 6393055
  28. Structure and polymorphism of the HLA class II SB light chain genes.
    EMBO J. 1984 Dec 1;3(12):2985-93 PMID: 6098459
  29. Transcription of the mouse dihydrofolate reductase gene proceeds unabated through seven polyadenylation sites and terminates near a region of repeated DNA.
    Mol Cell Biol. 1984 Dec;4(12):2921-4 PMID: 6084812
  30. Allelic polymorphism and complexity of the genes for HLA-DR beta-chains--direct analysis by DNA-DNA hybridization.
    Nature. 1982 Nov 25;300(5890):372-4 PMID: 6292729
  31. Polymorphism and complexity of the human DC and murine I-A alpha chain genes.
    EMBO J. 1983;2(1):121-4 PMID: 11894898
  32. Purification and structural characterisation of human HLA-linked B-cell antigens.
    Nature. 1977 Jul 21;268(5617):213-8 PMID: 301990
  33. Cloning in single-stranded bacteriophage as an aid to rapid DNA sequencing.
    J Mol Biol. 1980 Oct 25;143(2):161-78 PMID: 6260957
  34. Spacer DNA sequences upstream of the T-A-T-A-A-A-T-A sequence are essential for promotion of H2A histone gene transcription in vivo.
    Proc Natl Acad Sci U S A. 1980 Dec;77(12):7102-6 PMID: 6938957
  35. Family studies define a new histocompatibility locus, SB, between HLA-DR and GLO.
    Nature. 1981 Oct 29;293(5835):745-7 PMID: 6974826
  36. [Primary structure of class II human histocompatibility antigens. 1st communication. Amino acid sequence of the N-terminal 198 residues of the beta chain of a HLA-Dw2,2;DR2,2-alloantigen (author's transl)].
    Hoppe Seylers Z Physiol Chem. 1981 Dec;362(12):1665-9 PMID: 6947956
  37. Analysis of the HLA-linked SB gene system with cloned and uncloned alloreactive T-cell lines.
    Immunogenetics. 1982;15(2):187-98 PMID: 6174443
  38. cDNA clones coding for the heavy chain of human HLA-DR antigen.
    Proc Natl Acad Sci U S A. 1982 Jan;79(2):545-9 PMID: 6952207
  39. HLA-DR light chain has a polymorphic N-terminal region and a conserved immunoglobulin-like C-terminal region.
    Nature. 1982 Jun 24;297(5868):694-7 PMID: 6953314
  40. Nucleotide sequence of a portion of human chromosome 9 containing a leukocyte interferon gene cluster.
    J Mol Biol. 1982 Apr 15;156(3):467-86 PMID: 6181262
  41. HLA-DR antigens: structure, separation of subpopulations, gene cloning and function.
    Immunol Rev. 1982;66:133-87 PMID: 6813244
  42. Alpha and beta chains of SB and DR antigens are structurally distinct.
    J Exp Med. 1982 Nov 1;156(5):1557-62 PMID: 6182264
  43. Human class II major histocompatibility antigen beta-chains are derived from at least three loci.
    Nature. 1983 Jan 6;301(5895):82-4 PMID: 6296686
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/13.5.1607
Published
1985-03-11
Pages
1607-21
Language
English
Region
England
NLM ID
0411011
PMCID
PMC341099
Subset
IM
Databases
GENBANK
X02228
External Links
PubMed source DOI: 10.1093/nar/13.5.1607 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2