Home LiteratureArticle Details
PMID: 6206493 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Cloning and sequence analysis of the human major histocompatibility complex gene DC-3 beta.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 81 ·No. 16 ·1984-08-00 ·Pages 5199-203

Boss JM, Strominger JL

Abstract

The DC antigen is one of the class II major histocompatibility antigens involved in the regulation of the immune response. This molecule is a heterodimer composed of an alpha and a beta chain. Southern blot analysis of several homozygous cell lines shows that there are two DC beta genes. The DC-3 beta gene, corresponding to a polymorphic restriction fragment, was cloned and sequenced and found to exist in five exons spanning 8 kilobase pairs of DNA. These exons correspond to the functional domains of the DC beta protein. Comparison of the beta 1 domains of known DC beta chains shows that the polymorphism is clustered in four regions. A similar comparison of the mouse A beta sequences shows only two prominent diversity regions. The DC beta chain sequences are eight amino acids shorter than the A beta chain sequences due to the elimination of a small exon by an aberrant splice acceptor.

MeSH Terms
Base Sequence Cloning, Molecular Genes, MHC Class II Genes, Regulator Genetic Vectors Humans Major Histocompatibility Complex RNA/isolation & purification RNA, Messenger/genetics
Chemicals
RNA, Messenger RNA
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Boss J M
Strominger J L
References (30)
30 references, click to expand
  1. Genetic mapping of a human class II antigen beta-chain cDNA clone to the SB region of the HLA complex.
    Proc Natl Acad Sci U S A. 1983 Oct;80(19):6036-40 PMID: 6310612
  2. Primary structure of human class II histocompatibility antigens 3rd communication. Amino acid sequence comparison between DR and DC subclass antigens derived from a lymphoblastoid B cell line homozygous at the HLA loci (HLA-A3,3; B7,7; Dw2,2; DR2,2: MT1,1; Dc1,1: MB1,1).
    Hoppe Seylers Z Physiol Chem. 1983 Jun;364(6):749-55 PMID: 6576979
  3. NH2-terminal sequence of the alpha and beta chains of human DC-1 antigen isolated from the JY cell line. Homology with murine I-A molecules.
    Immunogenetics. 1983;18(5):453-9 PMID: 6417007
  4. Exon-intron organization and complete nucleotide sequence of a human major histocompatibility antigen DC beta gene.
    Proc Natl Acad Sci U S A. 1983 Dec;80(23):7313-7 PMID: 6316358
  5. The class II molecules of the human and murine major histocompatibility complex.
    Cell. 1984 Jan;36(1):1-13 PMID: 6198089
  6. Nucleotide sequence of mutant I-A beta bm12 gene is evidence for genetic exchange between mouse immune response genes.
    Nature. 1984 Apr 5-11;308(5959):551-3 PMID: 6324001
  7. Organization of the transcriptional unit of a human class II histocompatibility antigen: HLA-DR heavy chain.
    Nucleic Acids Res. 1983 Dec 20;11(24):8663-75 PMID: 6324094
  8. The nucleotide sequence of the murine I-E beta b immune response gene: evidence for gene conversion events in class II genes of the major histocompatibility complex.
    EMBO J. 1984 Jun;3(6):1221-5 PMID: 6086309
  9. Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose.
    Proc Natl Acad Sci U S A. 1972 Jun;69(6):1408-12 PMID: 4504350
  10. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
  11. Sequencing end-labeled DNA with base-specific chemical cleavages.
    Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
  12. A subcloning strategy for DNA sequence analysis.
    Nucleic Acids Res. 1980 Dec 11;8(23):5541-9 PMID: 6258146
  13. A system for shotgun DNA sequencing.
    Nucleic Acids Res. 1981 Jan 24;9(2):309-21 PMID: 6259625
  14. Cloning in single-stranded bacteriophage as an aid to rapid DNA sequencing.
    J Mol Biol. 1980 Oct 25;143(2):161-78 PMID: 6260957
  15. Gene conversion: some implications for immunoglobulin genes.
    Cell. 1981 Jun;24(3):592-4 PMID: 7018693
  16. Sequence of the yeast iso-1-cytochrome c mRNA.
    J Biol Chem. 1981 Dec 25;256(24):12958-61 PMID: 6273415
  17. The molecular characterization of three transcriptional mutations in the yeast iso-2-cytochrome c gene.
    J Biol Chem. 1982 Jul 10;257(13):7756-61 PMID: 6282853
  18. HLA-DR light chain has a polymorphic N-terminal region and a conserved immunoglobulin-like C-terminal region.
    Nature. 1982 Jun 24;297(5868):694-7 PMID: 6953314
  19. Complete amino acid sequence of an HLA-DR antigen-like beta chain as predicted from the nucleotide sequence: similarities with immunoglobulins and HLA-A, -B, and -C antigens.
    Proc Natl Acad Sci U S A. 1982 Jun;79(12):3687-91 PMID: 6954511
  20. HLA-DR antigens: structure, separation of subpopulations, gene cloning and function.
    Immunol Rev. 1982;66:133-87 PMID: 6813244
  21. The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers.
    Gene. 1982 Oct;19(3):259-68 PMID: 6295879
  22. Human class II major histocompatibility antigen beta-chains are derived from at least three loci.
    Nature. 1983 Jan 6;301(5895):82-4 PMID: 6296686
  23. The structure of a mutant H-2 gene suggests that the generation of polymorphism in H-2 genes may occur by gene conversion-like events.
    Nature. 1983 Feb 24;301(5902):671-4 PMID: 6828150
  24. The murine E alpha immune response gene.
    Cell. 1983 Mar;32(3):745-54 PMID: 6403249
  25. Murine I-A beta chain polymorphism: nucleotide sequences of three allelic I-A beta genes.
    Science. 1983 Jul 15;221(4607):283-6 PMID: 6407114
  26. Complete primary structures of the E beta chain and gene of the mouse major histocompatibility complex.
    Proc Natl Acad Sci U S A. 1983 Sep;80(18):5520-4 PMID: 6310581
  27. The complete nucleotide sequence of the I-E alpha d immune response gene.
    Nucleic Acids Res. 1983 Aug 11;11(15):5055-71 PMID: 6308570
  28. Regions of allelic hypervariability in the murine A alpha immune response gene.
    Cell. 1983 Aug;34(1):169-77 PMID: 6309407
  29. Structure of the murine immune response I-A beta locus: sequence of the I-A beta gene and an adjacent beta-chain second domain exon.
    Cell. 1983 Aug;34(1):179-88 PMID: 6411350
  30. Genes of the major histocompatibility complex in mouse and man.
    Science. 1983 Nov 18;222(4625):727-33 PMID: 6356354
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.81.16.5199
Published
1984-08-00
Pages
5199-203
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC391665
Subset
IM
Grants
NIAID NIH HHS · 1 F32 AI 06860 · United States
NIADDK NIH HHS · AM-30241 · United States
Databases
GENBANK
K02405
External Links
PubMed source DOI: 10.1073/pnas.81.16.5199 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2