Home LiteratureArticle Details
PMID: 6191198 Published · ppublish English Journal Article

Specific association of repetitive DNA sequences with major histocompatibility genes.

Molecular and cellular biology ·Vol. 3 ·No. 5 ·1983-05-00 ·Pages 903-13

Singer DS, Lifshitz R, Abelson L, Nyirjesy P, Rudikoff S

Abstract

The DNA sequence organization of a 17.8-kilobase segment of porcine DNA, containing a functional major histocompatibility (MHC) gene, has been studied. The DNA flanking the MHC gene contains at least 10 distinct repetitive DNA sequence elements, each of which occurs only once within the 17.8-kilobase DNA segment. Their reiteration frequencies in the genome range from 10(2) to 10(4). The genomic organization of seven of these sequence elements has been examined; all are interspersed with other, unrelated DNA sequences. These seven repeated sequences are not generally associated in the genome. However, they appear to be nonrandomly linked in MHC-associated regions of the genome: at least two additional DNA segments containing MHC-homologous DNA also contain sequences homologous to DNA fragments bearing the seven different repeats. Of the seven sequences, four can be detected in splenic total RNA. These results suggest that these repeated elements are specifically associated with the MHC locus.

MeSH Terms
Animals Cloning, Molecular DNA, Recombinant Major Histocompatibility Complex Nucleic Acid Hybridization RNA/genetics Repetitive Sequences, Nucleic Acid Swine Swine, Miniature/genetics
Chemicals
DNA, Recombinant RNA
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Singer D S
Lifshitz R
Abelson L
Nyirjesy P
Rudikoff S
References (40)
40 references, click to expand
  1. Analysis of repeating DNA sequences by reassociation.
    Methods Enzymol. 1974;29:363-418 PMID: 4850571
  2. Nucleotide sequence of the rightward operator of phage lambda.
    Proc Natl Acad Sci U S A. 1975 Mar;72(3):1184-8 PMID: 1055375
  3. Analysis of single- and double-stranded nucleic acids on polyacrylamide and agarose gels by using glyoxal and acridine orange.
    Proc Natl Acad Sci U S A. 1977 Nov;74(11):4835-8 PMID: 73185
  4. The protein products of the murine 17th chromosome: genetics and structure.
    Adv Immunol. 1978;26:147-93 PMID: 99995
  5. The isolation of structural genes from libraries of eucaryotic DNA.
    Cell. 1978 Oct;15(2):687-701 PMID: 719759
  6. Unusual organization of DNA sequences in the chicken.
    Biochemistry. 1978 Dec 26;17(26):5838-44 PMID: 728440
  7. The major histocompatibility complex of the mouse.
    Science. 1979 Feb 9;203(4380):516-21 PMID: 104386
  8. Regulation of gene expression: possible role of repetitive sequences.
    Science. 1979 Jun 8;204(4397):1052-9 PMID: 451548
  9. Prolonged incubation in calcium chloride improves the competence of Escherichia coli cells.
    Gene. 1979 May;6(1):23-8 PMID: 383576
  10. MHC-restricted cytotoxic T cells: studies on the biological role of polymorphic major transplantation antigens determining T-cell restriction-specificity, function, and responsiveness.
    Adv Immunol. 1979;27:51-177 PMID: 92183
  11. Complete amino acid sequence of a papain-solubilized human histocompatibility antigen, HLA-B7. 2. Sequence determination and search for homologies.
    Biochemistry. 1979 Dec 11;18(25):5711-20 PMID: 518865
  12. The clustered and scrambled arrangement of moderately repetitive elements in Drosophila DNA.
    Cell. 1979 Dec;18(4):1231-46 PMID: 117904
  13. A ubiquitous family of repeated DNA sequences in the human genome.
    J Mol Biol. 1979 Aug 15;132(3):289-306 PMID: 533893
  14. The organization of repetitive sequences in a cluster of rabbit beta-like globin genes.
    Cell. 1980 Feb;19(2):379-91 PMID: 6244107
  15. Complete amino acid sequence of pooled papain-solubilized HLA-A, -B, and -C antigens: relatedness to immunoglobulins and internal homologies.
    Proc Natl Acad Sci U S A. 1980 Feb;77(2):1129-33 PMID: 6928663
  16. Elution of DNA from agarose gels after electrophoresis.
    Methods Enzymol. 1979;68:176-82 PMID: 232211
  17. Plasmids of Escherichia coli as cloning vectors.
    Methods Enzymol. 1979;68:245-67 PMID: 232214
  18. Ubiquitous, interspersed repeated sequences in mammalian genomes.
    Proc Natl Acad Sci U S A. 1980 Mar;77(3):1398-402 PMID: 6246492
  19. HLA--a central immunological agency of man.
    Adv Hum Genet. 1980;10:137-208, 385-6 PMID: 6446849
  20. Purification of mouse immunoglobulin heavy-chain messenger RNAs from total myeloma tumor RNA.
    Eur J Biochem. 1980 Jun;107(2):303-14 PMID: 6772444
  21. Mouse rDNA nontranscribed spacer sequences are found flanking immunoglobulin CH genes and elsewhere throughout the genome.
    Cell. 1980 Nov;22(1 Pt 1):179-85 PMID: 6775816
  22. The nucleotide sequence of the ubiquitous repetitive DNA sequence B1 complementary to the most abundant class of mouse fold-back RNA.
    Nucleic Acids Res. 1980 Mar 25;8(6):1201-15 PMID: 7433120
  23. Cloning of V region fragments from mouse liver DNA and localization of repetitive DNA sequences in the vicinity of immunoglobulin gene segments.
    Nucleic Acids Res. 1980 Apr 25;8(8):1721-9 PMID: 6253945
  24. Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose.
    Proc Natl Acad Sci U S A. 1980 Sep;77(9):5201-5 PMID: 6159641
  25. Reiteration frequency mapping: analysis of repetitive sequence organization within cloned DNA fragments containing the human initiator methionine tRNA gene.
    Proc Natl Acad Sci U S A. 1980 Oct;77(10):5668-72 PMID: 6934502
  26. Molecular cloning of a human histocompatibility antigen cDNA fragment.
    Proc Natl Acad Sci U S A. 1980 Oct;77(10):6081-5 PMID: 6934534
  27. Primary structure of a murine transplantation antigen.
    Nature. 1981 May 7;291(5810):35-9 PMID: 7231522
  28. Locations of three repetitive sequence families found in BALB/c adult beta-globin clones.
    Nucleic Acids Res. 1981 Mar 11;9(5):1133-50 PMID: 7232212
  29. Three cDNA clones encoding mouse transplantation antigens: homology to immunoglobulin genes.
    Cell. 1981 Apr;24(1):125-34 PMID: 6786753
  30. Isolation and partial nucleotide sequence of a cDNA clone for human histocompatibility antigen HLA-B by use of an oligodeoxynucleotide primer.
    Proc Natl Acad Sci U S A. 1981 Jan;78(1):616-20 PMID: 6165999
  31. Primary structural: analysis of the transplantation antigens of the murine H-2 major histocompatibility complex.
    Annu Rev Biochem. 1981;50:1025-52 PMID: 7023355
  32. A chicken middle-repetitive DNA sequence which shares homology with mammalian ubiquitous repeats.
    Nucleic Acids Res. 1981 Oct 24;9(20):5383-97 PMID: 7301590
  33. Three classes of mouse H-2 messenger RNA distinguished by analysis of cDNA clones.
    Nature. 1982 Jan 7;295(5844):73-6 PMID: 6276751
  34. Exon/intron organization and complete nucleotide sequence of an HLA gene.
    Proc Natl Acad Sci U S A. 1982 Feb;79(3):893-7 PMID: 6461010
  35. Characterization of a porcine genomic clone encoding a major histocompatibility antigen: expression in mouse L cells.
    Proc Natl Acad Sci U S A. 1982 Mar;79(5):1403-7 PMID: 6461859
  36. SINEs and LINEs: highly repeated short and long interspersed sequences in mammalian genomes.
    Cell. 1982 Mar;28(3):433-4 PMID: 6280868
  37. Clusters of genes encoding mouse transplantation antigens.
    Cell. 1982 Mar;28(3):489-98 PMID: 6280871
  38. Organization of the constant-region gene family of the mouse immunoglobulin heavy chain.
    Cell. 1982 Mar;28(3):499-506 PMID: 6804095
  39. Structure and expression of a mouse major histocompatibility antigen gene, H-2Ld.
    Proc Natl Acad Sci U S A. 1982 Mar;79(6):1994-8 PMID: 6952248
  40. Tissue-specific expression of an unusual H-2 (class I)-related gene.
    Proc Natl Acad Sci U S A. 1982 Aug;79(16):4947-51 PMID: 6956903
Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.3.5.903-913.1983
Published
1983-05-00
Pages
903-13
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC368613
Subset
IM
External Links
PubMed source DOI: 10.1128/mcb.3.5.903-913.1983 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2