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PMID: 7232212 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Locations of three repetitive sequence families found in BALB/c adult beta-globin clones.

Nucleic acids research ·Vol. 9 ·No. 5 ·1981-03-11 ·Pages 1133-50

Haigwood NL, Jahn CL, Hutchison CA, Edgell MH

Abstract

Three different repeat sequences have been mapped within the cloned EcoRI fragments that contain the adult beta-globin genes from the BALB/c (Hddd) mouse. One sequence, "a", occurs 1.5-2 kb 3' to the beta-major gene. A second, "b", is found 4kb 5' and 7.5kb 3' to the beta-minor gene. The 14kb EcoRI fragment bearing the beta-minor gene carries at least one additional repetitive element, "c". Probing a BALB/c DNA library with each repeat has demonstrated that these sequences are moderately to highly repetitive and are extensively interspersed with each other throughout the genome. In addition, repeats "a" and "b" are preferentially found in satellite and main-band DNa, respectively. The occurrence of these repeats elsewhere in the beta-globin cluster was demonstrated by probing the non-adult globin clones with each repeat. The arrangement of these repeats around the non-adult genes is 5'-"b"-"b"-epsilon y-beta hl-beta h2-"c"-beta h3-3'. Probing the C57BL/10 (Hbbs) adult gene clones with these repeats demonstrated that the distribution of these sequences in the adult region of these two haplotypes is essentially the same.

MeSH Terms
Animals Base Sequence Cloning, Molecular DNA Genes Globins/genetics Male Mice Mice, Inbred BALB C/genetics Mice, Inbred C57BL Nucleic Acid Hybridization
Chemicals
Globins DNA
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Haigwood N L
Jahn C L
Hutchison C A
Edgell M H
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28 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/9.5.1133
Published
1981-03-11
Pages
1133-50
Language
English
Region
England
NLM ID
0411011
PMCID
PMC326742
Subset
IM
Grants
NIAID NIH HHS · AI08998 · United States
NIGMS NIH HHS · GM 21313 · United States
NIGMS NIH HHS · GM07121 · United States
External Links
PubMed source DOI: 10.1093/nar/9.5.1133 PMC Full Text View journal details
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