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PMID: 6952248 Published · ppublish English Journal Article

Structure and expression of a mouse major histocompatibility antigen gene, H-2Ld.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 79 ·No. 6 ·1982-03-00 ·Pages 1994-8

Evans GA, Margulies DH, Camerini-Otero RD, Ozato K, Seidman JG

Abstract

A genomic clone encoding H-2Ld, a mouse major transplantation antigen, has been identified and the structure of the H-2Ld gene has been partially determined. We isolated 35 genomic clones from a BALB/c (H-2d) genomic library by hybridization to mouse or human probes. One of these clones encodes H-2Ld as determined by two criteria. First, the gene encodes a protein that is identical at the 76 known amino acid positions for H-2Ld. Second, when introduced into L cells by DNA-mediated gene transfer, a new H-2 antigen is expressed that is recognized by anti-H-2Ld monoclonal antibodies. The sequence of the H-2Ld protein predicted by the DNA sequences shows more than 80% homology to known H-2 antigens. H-2L-like sequences are found in mutant H-2Kb molecules, suggesting that gene conversion or reciprocal recombination may play a role in the development of H-2 polymorphism.

MeSH Terms
Animals Base Sequence Biological Evolution Cloning, Molecular DNA/genetics Gene Conversion Gene Expression Regulation Genes H-2 Antigens/genetics Lymphoma/immunology Major Histocompatibility Complex Mice
Chemicals
H-2 Antigens DNA
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Evans G A
Margulies D H
Camerini-Otero R D
Ozato K
Seidman J G
References (34)
34 references, click to expand
  1. Charon phages: safer derivatives of bacteriophage lambda for DNA cloning.
    Science. 1977 Apr 8;196(4286):161-9 PMID: 847462
  2. The use of thin acrylamide gels for DNA sequencing.
    FEBS Lett. 1978 Mar 1;87(1):107-10 PMID: 631324
  3. Multiple related immunoglobulin variable-region genes identified by cloning and sequence analysis.
    Proc Natl Acad Sci U S A. 1978 Aug;75(8):3881-5 PMID: 279004
  4. The arrangement and rearrangement of antibody genes.
    Nature. 1978 Dec 21-28;276(5690):790-5 PMID: 103004
  5. The major histocompatibility complex of the mouse.
    Science. 1979 Feb 9;203(4380):516-21 PMID: 104386
  6. Transformation of mammalian cells with genes from procaryotes and eucaryotes.
    Cell. 1979 Apr;16(4):777-85 PMID: 222468
  7. Primary structural studies of an H-2L molecule confirm that it is a unique gene product with homology to H-2K and H-2D antigens.
    Proc Natl Acad Sci U S A. 1980 Feb;77(2):1134-8 PMID: 6928664
  8. Functional studies of the products of H-2L locus.
    Immunogenetics. 1980;10(1):7-17 PMID: 6967857
  9. Cloned human and mouse kappa immunoglobulin constant and J region genes conserve homology in functional segments.
    Cell. 1980 Nov;22(1 Pt 1):197-207 PMID: 6775818
  10. Phenotypic expression of I-A and I-E/C subregion determinants on murine thymocytes.
    J Immunol. 1980 Nov;125(5):2263-8 PMID: 6159403
  11. Monoclonal antibodies to mouse MHC antigens. II. Antibodies to the H-2Ld antigen, the products of a third polymorphic locus of the mouse major histocompatibility complex.
    J Immunol. 1980 Dec;125(6):2473-7 PMID: 7191868
  12. Nucleosomal packaging of the thymidine kinase gene of herpes simplex virus transferred into mouse cells: an actively expressed single-copy gene.
    Proc Natl Acad Sci U S A. 1980 Sep;77(9):5079-83 PMID: 6254055
  13. Molecular cloning of a human histocompatibility antigen cDNA fragment.
    Proc Natl Acad Sci U S A. 1980 Oct;77(10):6081-5 PMID: 6934534
  14. Monoclonal antibodies to mouse MHC antigens. III. Hybridoma antibodies reacting to antigens of the H-2b haplotype reveal genetic control of isotype expression.
    J Immunol. 1981 Jan;126(1):317-21 PMID: 6935293
  15. Human immunoglobulin variable region genes--DNA sequences of two V kappa genes and a pseudogene.
    Nature. 1980 Dec 25;288(5792):730-3 PMID: 6779204
  16. Primary structure of murine major histocompatibility complex alloantigens. Amino acid sequence of the NH2-terminal ninety-eight residues of the H-2Db glycoprotein.
    J Biol Chem. 1981 Mar 25;256(6):2863-72 PMID: 7225204
  17. A system for shotgun DNA sequencing.
    Nucleic Acids Res. 1981 Jan 24;9(2):309-21 PMID: 6259625
  18. Biochemistry of the gene products from murine MHC mutants.
    Annu Rev Genet. 1980;14:241-77 PMID: 7011174
  19. Cloning in single-stranded bacteriophage as an aid to rapid DNA sequencing.
    J Mol Biol. 1980 Oct 25;143(2):161-78 PMID: 6260957
  20. Primary structure of a murine transplantation antigen.
    Nature. 1981 May 7;291(5810):35-9 PMID: 7231522
  21. Three cDNA clones encoding mouse transplantation antigens: homology to immunoglobulin genes.
    Cell. 1981 Apr;24(1):125-34 PMID: 6786753
  22. Major histocompatibility antigens: the human (HLA-A, -B, -C) and murine (H-2K, H-2D) class I molecules.
    Cell. 1981 May;24(2):287-99 PMID: 7016338
  23. Isolation and partial nucleotide sequence of a cDNA clone for human histocompatibility antigen HLA-B by use of an oligodeoxynucleotide primer.
    Proc Natl Acad Sci U S A. 1981 Jan;78(1):616-20 PMID: 6165999
  24. Mouse beta 2-microglobulin cDNA clones: a screening procedure for cDNA clones corresponding to rare mRNAs.
    Proc Natl Acad Sci U S A. 1981 Apr;78(4):2253-7 PMID: 6166005
  25. Gene conversion: some implications for immunoglobulin genes.
    Cell. 1981 Jun;24(3):592-4 PMID: 7018693
  26. Mechanisms and applications of DNA-mediated gene transfer in mammalian cells - a review.
    Gene. 1981 Jun-Jul;14(1-2):1-10 PMID: 7262562
  27. Structure of C-terminal half of two H-2 antigens from cloned mRNA.
    Nature. 1981 Jul 2;292(5818):78-81 PMID: 6895103
  28. A pseudogene homologous to mouse transplantation antigens: transplantation antigens are encoded by eight exons that correlate with protein domains.
    Cell. 1981 Sep;25(3):683-92 PMID: 6895187
  29. H-2-like genes in the Tla region of mouse chromosome 17.
    Nature. 1982 Jan 14;295(5845):168-70 PMID: 6276757
  30. Identification of a BALB/c H-2Ld gene by DNA-mediated gene transfer.
    Science. 1982 Feb 5;215(4533):677-9 PMID: 7058331
  31. DNA sequence of a gene encoding a BALB/c mouse Ld transplantation antigen.
    Science. 1982 Feb 5;215(4533):679-82 PMID: 7058332
  32. SEQ: a nucleotide sequence analysis and recombination system.
    Nucleic Acids Res. 1982 Jan 11;10(1):279-94 PMID: 7063402
  33. Enhanced graphic matrix analysis of nucleic acid and protein sequences.
    Proc Natl Acad Sci U S A. 1981 Dec;78(12):7665-9 PMID: 6801656
  34. STUDIES ON INDUCED RESISTANCE AGAINST ISOTRANSPLANTS OF VIRUS-INDUCED LEUKEMIA.
    Cancer Res. 1964 Apr;24:502-8 PMID: 14147826
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.79.6.1994
Published
1982-03-00
Pages
1994-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC346108
Subset
IM
Databases
GENBANK
J00403, J00404, L00127, L00128, L00129
External Links
PubMed source DOI: 10.1073/pnas.79.6.1994 PMC Full Text View journal details
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