Home LiteratureArticle Details
PMID: 6254055 Published · ppublish English Journal Article

Nucleosomal packaging of the thymidine kinase gene of herpes simplex virus transferred into mouse cells: an actively expressed single-copy gene.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 77 ·No. 9 ·1980-09-00 ·Pages 5079-83

Camerini-Otero RD, Zasloff MA

Abstract

We have studied the nucleoprotein structure of the herpes thymidine kinase gene introduced into mouse Ltk-aprt- cells by means of DNA-mediated gene transfer. Using the technique of Southern blotting, we examined staphylococcal digests of the nuclei from the relatively stable transformants that contain one or less integrated copies of the thymidine kinase gene per haploid genome. Out experiments show that, under selection for the active expression of this gene, it is packaged in nucleosomes with a repeat length identical to the average for the host mouse sequences.

MeSH Terms
Animals Cell Line DNA/metabolism DNA Restriction Enzymes DNA, Viral/metabolism Electrophoresis, Agar Gel Genes, Viral L Cells Mice Nucleosomes/analysis Recombination, Genetic Simplexvirus/genetics Thymidine Kinase/genetics Transformation, Genetic
Chemicals
DNA, Viral Nucleosomes DNA Thymidine Kinase DNA Restriction Enzymes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Camerini-Otero R D
Zasloff M A
References (43)
43 references, click to expand
  1. Transfer of thymidine kinase to thymidine kinaseless L cells by infection with ultraviolet-irradiated herpes simplex virus.
    J Virol. 1971 Jun;7(6):813-20 PMID: 4327589
  2. The chromosome of bacteriophage T5. I. Analysis of the single-stranded DNA fragments by agarose gel electrophoresis.
    J Mol Biol. 1972 Feb 14;63(3):383-95 PMID: 5014925
  3. A new technique for the assay of infectivity of human adenovirus 5 DNA.
    Virology. 1973 Apr;52(2):456-67 PMID: 4705382
  4. Phosphorylation of 5-bromodeoxycytidine in cells infected with herpes simplex virus.
    Proc Natl Acad Sci U S A. 1973 Dec;70(12):3788-92 PMID: 4359489
  5. Nucleotide sequence of the rightward operator of phage lambda.
    Proc Natl Acad Sci U S A. 1975 Mar;72(3):1184-8 PMID: 1055375
  6. Temperature-sensitive mutants of herpes simplex virus type 1 defective in lysis but not in transformation.
    J Virol. 1975 Aug;16(2):275-83 PMID: 168402
  7. Folding of the DNA double helix in chromatin-like structures from simian virus 40.
    Proc Natl Acad Sci U S A. 1975 May;72(5):1843-7 PMID: 168578
  8. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
  9. Analysis of DNA of isolated chromatin subunits.
    Proc Natl Acad Sci U S A. 1975 Oct;72(10):3978-82 PMID: 1060078
  10. Chromatin-like organization of the adenovirus chromosome.
    Proc Natl Acad Sci U S A. 1976 Feb;73(2):401-4 PMID: 1061143
  11. Human deoxythymidine kinase. I. Purification and general properties of the cytoplasmic and mitochondrial isozymes derived from blast cells of acute myelocytic leukemia.
    J Biol Chem. 1976 May 10;251(9):2600-4 PMID: 1063125
  12. Chromosomal subunits in active genes have an altered conformation.
    Science. 1976 Sep 3;193(4256):848-56 PMID: 948749
  13. The organization of histones and DNA in chromatin: evidence for an arginine-rich histone kernel.
    Cell. 1976 Jul;8(3):333-47 PMID: 986252
  14. Selective digestion of transcriptionally active ovalbumin genes from oviduct nuclei.
    Proc Natl Acad Sci U S A. 1976 Nov;73(11):3966-70 PMID: 1069279
  15. Stability of nucleosomes in native and reconstituted chromatins.
    Nucleic Acids Res. 1976 Nov;3(11):3173-92 PMID: 188018
  16. A comparison of the structure of chicken erythrocyte and chicken liver chromatin.
    Cell. 1976 Dec;9(4 Pt 1):627-32 PMID: 1009580
  17. Transfer of the gene for thymidine kinase to thymidine kinase-deficient human cells by purified herpes simplex viral DNA.
    Proc Natl Acad Sci U S A. 1977 Apr;74(4):1590-4 PMID: 193108
  18. Variation in chromatin structure in two cell types from the same tissue: a short DNA repeat length in cerebral cortex neurons.
    Cell. 1977 Apr;10(4):633-40 PMID: 862024
  19. Transfer of purified herpes virus thymidine kinase gene to cultured mouse cells.
    Cell. 1977 May;11(1):223-32 PMID: 194704
  20. Biochemical transformation of mouse cells by fragments of herpes simplex virus DNA.
    Cell. 1977 May;11(1):233-41 PMID: 194705
  21. Chromatin.
    Nature. 1978 Jan 12;271(5641):115-22 PMID: 340956
  22. A new method for the purification and identification of covalently closed circular DNA molcules.
    Nucleic Acids Res. 1978 Apr;5(4):1139-52 PMID: 26042
  23. The transfer and stable integration of the HSV thymidine kinase gene into mouse cells.
    Cell. 1978 May;14(1):133-41 PMID: 208776
  24. Nucleosome structure III: the structure and transcriptional activity of the chromatin containing the ovalbumin and globin genes in chick oviduct nuclei.
    Cold Spring Harb Symp Quant Biol. 1978;42 Pt 2:779-91 PMID: 277317
  25. Biochemical transfer of single-copy eucaryotic genes using total cellular DNA as donor.
    Cell. 1978 Jul;14(3):725-31 PMID: 210957
  26. Fractionation of hen oviduct chromatin into transcriptionally active and inactive regions after selective micrococcal nuclease digestion.
    Cell. 1978 Sep;15(1):141-50 PMID: 699037
  27. A rational approach to the development of antiviral chemotherapy: alternative substrates of herpes simplex virus Type 1 (HSV-1) and Type 2 (HSV-2) thymidine kinase (TK).
    Ann N Y Acad Sci. 1977 Mar 4;284:594-8 PMID: 212988
  28. Preparative and analytical purification of DNA from agarose.
    Proc Natl Acad Sci U S A. 1979 Feb;76(2):615-9 PMID: 284385
  29. Nucleosomal structure of Epstein-Barr virus DNA in transformed cell lines.
    J Virol. 1979 Feb;29(2):657-65 PMID: 219253
  30. Random cleavage of intranuclear herpes simplex virus DNA by micrococcal nuclease.
    FEBS Lett. 1979 Apr 1;100(1):107-9 PMID: 220083
  31. DNA-mediated transfer of the adenine phosphoribosyltransferase locus into mammalian cells.
    Proc Natl Acad Sci U S A. 1979 Mar;76(3):1373-6 PMID: 286319
  32. Transformation of mammalian cells with genes from procaryotes and eucaryotes.
    Cell. 1979 Apr;16(4):777-85 PMID: 222468
  33. The chromatin structure of specific genes: II. Disruption of chromatin structure during gene activity.
    Cell. 1979 Apr;16(4):807-14 PMID: 455450
  34. Parental adenovirus DNA accumulates in nucleosome-like structures in infected cells.
    Nucleic Acids Res. 1979 Jun 25;6(8):2769-85 PMID: 461205
  35. Chromatin structure of the 5S ribonucleic acid genes of Xenopus laevis.
    Biochemistry. 1979 Jul 24;18(15):3223-31 PMID: 465464
  36. Construction and characterization of a recombinant plasmid encoding the gene for the thymidine kinase of Herpes simplex type 1 virus.
    Gene. 1979 Nov;7(3-4):335-42 PMID: 230124
  37. Introduction and expression of a rabbit beta-globin gene in mouse fibroblasts.
    Proc Natl Acad Sci U S A. 1979 Nov;76(11):5684-8 PMID: 293672
  38. DNA-mediated gene transfer of a circular plasmid into murine cells.
    Proc Natl Acad Sci U S A. 1979 Nov;76(11):5820-4 PMID: 293687
  39. Studies on the mechanism of transcription of nucleosomal complexes.
    Eur J Biochem. 1980 Jan;103(2):219-26 PMID: 6244943
  40. Limited DNase I nicking as a probe of gene conformation.
    Proc Natl Acad Sci U S A. 1980 Apr;77(4):1907-11 PMID: 6929527
  41. Transformation of the gene for hypoxanthine phosphoribosyltransferase.
    Somatic Cell Genet. 1979 Nov;5(6):1031-44 PMID: 397622
  42. Rabbit beta-globin mRNA production in mouse L cells transformed with cloned rabbit beta-globin chromosomal DNA.
    Nature. 1979 Sep 6;281(5726):40-6 PMID: 551266
  43. DELETION OF THYMIDINE KINASE ACTIVITY FROM L CELLS RESISTANT TO BROMODEOXYURIDINE.
    Exp Cell Res. 1963 Aug;31:297-312 PMID: 14056989
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.77.9.5079
Published
1980-09-00
Pages
5079-83
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC349999
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.77.9.5079 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2