Stability of nucleosomes in native and reconstituted chromatins.

Nucleic acids research ·Vol. 3 ·No. 11 ·1976-11-00 ·Pages 3173-92

Germond JE, Bellard M, Oudet P, Chambon P

Abstract

The stability of nucleosomes of SV40 minichromosomes extracted from infected cells or reconstituted by association of SV40 DNA and the four histones H2A, H2B, H3 and H4 was studied as a function of the ionic strength. As a measure of the stability of the nucleosome, we followed the disappearance of the nucleosomes from the original chromatin and their appearance on a "competing" DNA. We show here that the DNA and the histone components of the nucleosomes do not apprecially dissociate below 800 mM NaCl. At 800 mM and above, the histone moiety of the nucleosomes can dissociate from the DNA and efficiently participate to the formation of nucleosomes on a "competing" DNA.

MeSH Terms

Animals Cattle Cell Nucleus/ultrastructure Chromatin/ultrastructure DNA DNA, Circular DNA, Viral Drug Stability Electrophoresis, Polyacrylamide Gel Histones Macromolecular Substances Microscopy, Electron Molecular Weight Nucleic Acid Conformation Osmolar Concentration Simian virus 40/ultrastructure Thymus Gland

Chemicals

Chromatin DNA, Circular DNA, Viral Histones Macromolecular Substances DNA

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Germond J E

Bellard M

Oudet P

Chambon P

References (7)

7 references, click to expand

Electron microscopic and biochemical evidence that chromatin structure is a repeating unit.
Cell. 1975 Apr;4(4):281-300 PMID: 1122558
Folding of the DNA double helix in chromatin-like structures from simian virus 40.
Proc Natl Acad Sci U S A. 1975 May;72(5):1843-7 PMID: 168578
Stepwise relaxation of supercoiled SV40 DNA.
Cold Spring Harb Symp Quant Biol. 1975;39 Pt 1:199-208 PMID: 169060
Deficiency in histone acetylation in nontransforming host range mutants of polyoma virus.
Proc Natl Acad Sci U S A. 1976 Apr;73(4):1092-6 PMID: 177973
An octamer of histones in chromatin and free in solution.
Proc Natl Acad Sci U S A. 1975 Jul;72(7):2626-30 PMID: 241077
Structure of chromatin.
Nat New Biol. 1971 Jan 27;229(4):101-6 PMID: 5283616
High resolution acrylamide gel electrophoresis of histones.
Arch Biochem Biophys. 1969 Mar;130(1):337-46 PMID: 5778650

Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/3.11.3173

Published

1976-11-00

Pages

3173-92

Language

English

Region

England

NLM ID

0411011

PMCID

PMC343161

Subset

External Links

PubMed source DOI: 10.1093/nar/3.11.3173 PMC Full Text View journal details

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Stability of nucleosomes in native and reconstituted chromatins.

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