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PMID: 461205 Published · ppublish English Journal Article

Parental adenovirus DNA accumulates in nucleosome-like structures in infected cells.

Nucleic acids research ·Vol. 6 ·No. 8 ·1979-06-25 ·Pages 2769-85

Tate VE, Philipson L

Abstract

Micrococcal-nuclease digestion of adenovirus 2(ad 2) infected HeLa cell nuclei early after infection has been used to investigate the nucleoprotein nature of parental viral DNA. Viral DNA is more susceptible to nuclease digestion than cellular DNA. The pattern of digestion products changes as digestion proceeds from an indistinct pattern 1 hour post infection(pi) to a nucleosome-like pattern at 6 hours pi. The major differences between viral and cellular nucleoprotein products were i) a subnucleosome fraction from viral DNA and ii) the repeat size of DNA in viral nucleosomes was 165 base pairs and in cellular nucleosomes, 195 base pairs. Up to 50% viral DNA in nuclei 6 hours pi seems to be in nucleosome-like structures. Such patterns are not seen on digestion of partially-uncoated virus or isolated cores.

MeSH Terms
Adenoviruses, Human/metabolism Cell Nucleus/metabolism DNA Replication DNA, Viral/biosynthesis HeLa Cells/metabolism Humans Molecular Weight Nucleic Acid Hybridization Virus Replication
Chemicals
DNA, Viral
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Tate V E
Philipson L
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35 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/6.8.2769
Published
1979-06-25
Pages
2769-85
Language
English
Region
England
NLM ID
0411011
PMCID
PMC327892
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/6.8.2769 PMC Full Text View journal details
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