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PMID: 2987816 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Drosophila topoisomerase II double-strand DNA cleavage: analysis of DNA sequence homology at the cleavage site.

Nucleic acids research ·Vol. 13 ·No. 4 ·1985-02-25 ·Pages 1057-72

Sander M, Hsieh TS

Abstract

In order to study the sequence specificity of double-strand DNA cleavage by Drosophila topoisomerase II, we have mapped and sequenced 16 strong and 47 weak cleavage sites in the recombinant plasmid p pi 25.1. Analysis of the nucleotide and dinucleotide frequencies in the region near the site of phosphodiester bond breakage revealed a nonrandom distribution. The nucleotide frequencies observed would occur by chance with a probability less than 0.05. The consensus sequence we derived is 5'GT.A/TAY decrease ATT.AT..G 3', where a dot means no preferred nucleotide, Y is for pyrimidine, and the arrow shows the point of bond cleavage. On average, strong sites match the consensus better than weak sites.

MeSH Terms
Animals Base Sequence Chromosome Mapping DNA/metabolism DNA Topoisomerases, Type II/metabolism DNA, Recombinant Drosophila melanogaster/enzymology,genetics Plasmids Substrate Specificity
Chemicals
DNA, Recombinant DNA DNA Topoisomerases, Type II
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Sander M
Hsieh T S
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32 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/13.4.1057
Published
1985-02-25
Pages
1057-72
Language
English
Region
England
NLM ID
0411011
PMCID
PMC341056
Subset
IM
Grants
NIGMS NIH HHS · GM29006 · United States
External Links
PubMed source DOI: 10.1093/nar/13.4.1057 PMC Full Text View journal details
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