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PMID: 2501782 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Multiple enhancer-like sequences in the HLA-B7 gene.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 86 ·No. 14 ·1989-07-00 ·Pages 5247-51

Ganguly S, Vasavada HA, Weissman SM

Abstract

Enhancer-like sequences have previously been identified in the promoter region of the mouse major histocompatibility complex (MHC) class I genes. We have screened for such sequences in and around a human MHC class I gene, HLA-B7. Various restriction fragments of the B7 gene were assayed for their ability to enhance transcription of a bacterial chloramphenicol acetyltransferase gene from a simian virus 40 promoter in transiently transfected mouse LTA cells. Our results demonstrate that enhancer activity is located in introns 3 and 5 as well as 5' to the transcription initiation site. RNase protection experiments corroborate the results. Preliminary experiments indicate that B7 enhancers are active in various cell types. The role of these enhancers in B7 gene expression is not known at present. We speculate that the position of the enhancer elements may be related to the occurrence of Hpa II tiny fragment islands.

MeSH Terms
Animals Base Sequence Chloramphenicol O-Acetyltransferase/genetics Enhancer Elements, Genetic Genes Genes, MHC Class I HLA-B Antigens/genetics HLA-B7 Antigen HeLa Cells/immunology Humans L Cells/immunology Mice Molecular Sequence Data Plasmids Restriction Mapping Transfection beta-Galactosidase/genetics
Chemicals
HLA-B Antigens HLA-B7 Antigen Chloramphenicol O-Acetyltransferase beta-Galactosidase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Ganguly S
Department of Human Genetics, Yale University School of Medicine, New Haven, CT 06510.
Vasavada H A
Weissman S M
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.86.14.5247
Published
1989-07-00
Pages
5247-51
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC297598
Subset
IM
Grants
NCI NIH HHS · CA 42556-03 · United States
External Links
PubMed source DOI: 10.1073/pnas.86.14.5247 PMC Full Text View journal details
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