Home LiteratureArticle Details
PMID: 15238208 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. Review

The NADPH oxidase of professional phagocytes--prototype of the NOX electron transport chain systems.

Biochimica et biophysica acta ·Vol. 1657 ·No. 1 ·2004-06-28 ·Pages 1-22

Cross AR, Segal AW

Abstract

The NADPH oxidase is an electron transport chain in "professional" phagocytic cells that transfers electrons from NADPH in the cytoplasm, across the wall of the phagocytic vacuole, to form superoxide. The electron transporting flavocytochrome b is activated by the integrated function of four cytoplasmic proteins. The antimicrobial function of this system involves pumping K+ into the vacuole through BKCa channels, the effect of which is to elevate the vacuolar pH and activate neutral proteases. A number of homologous systems have been discovered in plants and lower animals as well as in man. Their function remains to be established.

MeSH Terms
Animals Electron Transport Chain Complex Proteins/chemistry,immunology,metabolism Enzyme Activation Humans Isoenzymes/metabolism NADPH Oxidases/chemistry,immunology,metabolism Phagocytes/enzymology,immunology Respiratory Burst/physiology
Chemicals
Electron Transport Chain Complex Proteins Isoenzymes NADPH Oxidases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Cross Andrew R
Department of Molecular and Experimental Medicine, The Scripps Research Institute, 10550 North Torrey Pines Road, La Jolla, CA 92037, USA.
Segal Anthony W
References (322)
322 references, click to expand
  1. High-conductance calcium-activated potassium channels; structure, pharmacology, and function.
    J Bioenerg Biomembr. 1996 Jun;28(3):255-67 PMID: 8807400
  2. Electron transfer across the O2- generating flavocytochrome b of neutrophils. Evidence for a transition from a low-spin state to a high-spin state of the heme iron component.
    Biochemistry. 1996 Oct 15;35(41):13400-10 PMID: 8873608
  3. Superoxide-producing cytochrome b. Enzymatic and electron paramagnetic resonance properties of cytochrome b558 purified from neutrophils.
    J Biol Chem. 1993 Feb 25;268(6):4025-31 PMID: 8382686
  4. Evidence for small intracellular vesicles in human blood phagocytes containing cytochrome b558 and the adhesion molecule CD11b/CD18.
    Blood. 1993 Jun 1;81(11):3122-9 PMID: 8098969
  5. Generation of superoxide by purified and relipidated cytochrome b559 in the absence of cytosolic activators.
    FEBS Lett. 1993 Jul 19;327(1):57-62 PMID: 8392946
  6. Electron currents generated by the human phagocyte NADPH oxidase.
    Nature. 1998 Apr 16;392(6677):734-7 PMID: 9565037
  7. Virulence of catalase-deficient aspergillus nidulans in p47(phox)-/- mice. Implications for fungal pathogenicity and host defense in chronic granulomatous disease.
    J Clin Invest. 1998 May 1;101(9):1843-50 PMID: 9576747
  8. Six Arabidopsis thaliana homologues of the human respiratory burst oxidase (gp91phox).
    Plant J. 1998 May;14(3):365-70 PMID: 9628030
  9. Gp91(phox) is the heme binding subunit of the superoxide-generating NADPH oxidase.
    Proc Natl Acad Sci U S A. 1998 Jul 7;95(14):7993-8 PMID: 9653128
  10. Role of NADH/NADPH oxidase-derived H2O2 in angiotensin II-induced vascular hypertrophy.
    Hypertension. 1998 Sep;32(3):488-95 PMID: 9740615
  11. Conformational changes of the leukocyte NADPH oxidase subunit p47(phox) during activation studied through its intrinsic fluorescence.
    Biochim Biophys Acta. 1998 Sep 8;1387(1-2):406-14 PMID: 9748657
  12. Inside the neutrophil phagosome: oxidants, myeloperoxidase, and bacterial killing.
    Blood. 1998 Nov 1;92(9):3007-17 PMID: 9787133
  13. p40(phox) is phosphorylated on threonine 154 and serine 315 during activation of the phagocyte NADPH oxidase. Implication of a protein kinase c-type kinase in the phosphorylation process.
    J Biol Chem. 1998 Nov 13;273(46):30097-103 PMID: 9804763
  14. Activation of NADPH oxidase involves the dissociation of p21rac from its inhibitory GDP/GTP exchange protein (rhoGDI) followed by its translocation to the plasma membrane.
    Biochem J. 1994 Mar 15;298 Pt 3:585-91 PMID: 8141770
  15. A point mutation in gp91-phox of cytochrome b558 of the human NADPH oxidase leading to defective translocation of the cytosolic proteins p47-phox and p67-phox.
    J Clin Invest. 1994 May;93(5):2120-6 PMID: 8182143
  16. Interaction of Rac with p67phox and regulation of phagocytic NADPH oxidase activity.
    Science. 1994 Jul 22;265(5171):531-3 PMID: 8036496
  17. The Ca2+/NADPH-dependent H2O2 generator in thyroid plasma membrane: inhibition by diphenyleneiodonium.
    Biochem J. 1994 Jul 1;301 ( Pt 1):75-81 PMID: 8037694
  18. The superoxide-generating system of human neutrophils possesses a novel diaphorase activity. Evidence for distinct regulation of electron flow within NADPH oxidase by p67-phox and p47-phox.
    J Biol Chem. 1994 Aug 26;269(34):21448-54 PMID: 8063777
  19. Chronic granulomatous disease.
    Biochim Biophys Acta. 1994 Oct 21;1227(1-2):1-24 PMID: 7918677
  20. Assembly of the phagocyte NADPH oxidase: binding of Src homology 3 domains to proline-rich targets.
    Proc Natl Acad Sci U S A. 1994 Oct 25;91(22):10650-4 PMID: 7938008
  21. Assessment of the contribution of the cytochrome b moiety of the NADPH oxidase to the transmembrane H+ conductance of leukocytes.
    J Biol Chem. 1994 Nov 4;269(44):27280-5 PMID: 7525551
  22. Characterization and partial purification of a novel neutrophil membrane-associated kinase capable of phosphorylating the respiratory burst component p47phox.
    Biochem J. 1999 Mar 1;338 ( Pt 2):359-66 PMID: 10024511
  23. A ferric-chelate reductase for iron uptake from soils.
    Nature. 1999 Feb 25;397(6721):694-7 PMID: 10067892
  24. Severe impairment in early host defense against Candida albicans in mice deficient in myeloperoxidase.
    Infect Immun. 1999 Apr;67(4):1828-36 PMID: 10085024
  25. High-conductance calcium-activated potassium channels in rat brain: pharmacology, distribution, and subunit composition.
    Biochemistry. 1999 Apr 27;38(17):5392-400 PMID: 10220326
  26. NADPH dehydrogenase activity of p67PHOX, a cytosolic subunit of the leukocyte NADPH oxidase.
    Biochemistry. 1999 May 4;38(18):5746-53 PMID: 10231525
  27. Transient association of the nicotinamide adenine dinucleotide phosphate oxidase subunits p47phox and p67phox with phagosomes in neutrophils from patients with X-linked chronic granulomatous disease.
    Blood. 1999 May 15;93(10):3521-30 PMID: 10233905
  28. Phosphorylation of p67phox in the neutrophil occurs in the cytosol and is independent of p47phox.
    FEBS Lett. 1999 Apr 23;449(2-3):225-9 PMID: 10338137
  29. Simultaneous presence of p47(phox) and flavocytochrome b-245 are required for the activation of NADPH oxidase by anionic amphiphiles. Evidence for an intermediate state of oxidase activation.
    J Biol Chem. 1999 May 28;274(22):15519-25 PMID: 10336445
  30. The tumour suppressor protein VHL targets hypoxia-inducible factors for oxygen-dependent proteolysis.
    Nature. 1999 May 20;399(6733):271-5 PMID: 10353251
  31. The participation of the hemes of flavocytochrome b245 in the electron transfer process in NADPH oxidase.
    Blood. 1999 Jun 15;93(12):4449 PMID: 10391701
  32. The mechanism of activation of NADPH oxidase in the cell-free system: the activation process is primarily catalytic and not through the formation of a stoichiometric complex.
    Biochem J. 1999 Jul 15;341 ( Pt 2):251-5 PMID: 10393079
  33. O2 sensing is preserved in mice lacking the gp91 phox subunit of NADPH oxidase.
    Proc Natl Acad Sci U S A. 1999 Jul 6;96(14):7944-9 PMID: 10393927
  34. Biochemical characterization of a Ca2+/NAD(P)H-dependent H2O2 generator in human thyroid tissue.
    Biochimie. 1999 Apr;81(4):373-80 PMID: 10401672
  35. The p67(phox) activation domain regulates electron flow from NADPH to flavin in flavocytochrome b(558).
    J Biol Chem. 1999 Aug 13;274(33):22999-3005 PMID: 10438466
  36. A noninvasive fluorimetric procedure for measurement of membrane potential. Quantification of the NADPH oxidase-induced depolarization in activated neutrophils.
    J Biol Chem. 1999 Sep 10;274(37):26098-104 PMID: 10473559
  37. Cell transformation by the superoxide-generating oxidase Mox1.
    Nature. 1999 Sep 2;401(6748):79-82 PMID: 10485709
  38. Functional analysis of NADPH oxidase in granulocytic cells expressing a delta488-497 gp91(phox) deletion mutant.
    Blood. 1999 Oct 1;94(7):2497-504 PMID: 10498623
  39. The active form of the ferric heme in neutrophil cytochrome b(558) is low-spin in the reconstituted cell-free system in the presence of amphophil.
    J Biochem. 1999 Oct;126(4):708-14 PMID: 10502679
  40. Protein kinase C (PKC) isoforms translocate to Triton-insoluble fractions in stimulated human neutrophils: correlation of conventional PKC with activation of NADPH oxidase.
    J Immunol. 1999 Oct 15;163(8):4574-82 PMID: 10510401
  41. Demonstration by the Nadi reaction of cytochrome oxidase activity in cells of the lymphoid and myeloid series, obtained from normal individuals and patients with Hodgkin's disease.
    Am J Pathol. 1951 Jul-Aug;27(4):738-9 PMID: 14846963
  42. The biochemical basis of phagocytosis. I. Metabolic changes during the ingestion of particles by polymorphonuclear leukocytes.
    J Biol Chem. 1959 Jun;234(6):1355-62 PMID: 13654378
  43. Studies on the labile, stable Nadi oxidase and peroxidase staining reactions in the isolated particles of horse granulocyte.
    Nagoya J Med Sci. 1961 May;23:362-78 PMID: 13712255
  44. Electrical properties of mitochondrial membranes.
    J Biophys Biochem Cytol. 1960 Jul;7:589-601 PMID: 14431035
  45. Cytosolic phox proteins interact with and regulate the assembly of coronin in neutrophils.
    J Cell Sci. 1997 Dec;110 ( Pt 24):3071-81 PMID: 9365277
  46. Assembly of the neutrophil respiratory burst oxidase. Protein kinase C promotes cytoskeletal and membrane association of cytosolic oxidase components.
    J Biol Chem. 1991 Mar 25;266(9):5911-7 PMID: 1848559
  47. Photoaffinity labeling and photoinactivation of the O2(-)-generating oxidase of neutrophils by an azido derivative of FAD.
    Biochemistry. 1995 Feb 7;34(5):1760-70 PMID: 7849036
  48. The cytosolic activating factors p47phox and p67phox have distinct roles in the regulation of electron flow in NADPH oxidase.
    J Biol Chem. 1995 Mar 24;270(12):6543-8 PMID: 7896790
  49. The mechanism of electron donation to molecular oxygen by phagocytic cytochrome b558.
    J Biol Chem. 1995 Apr 7;270(14):7853-7 PMID: 7713877
  50. A variant X-linked chronic granulomatous disease patient (X91+) with partially functional cytochrome b.
    J Biol Chem. 1995 Apr 7;270(14):8194-200 PMID: 7713925
  51. Kinetic characterization of the redox components in solubilized membranes from porcine neutrophils: reduction with dithionite and photoexcited NAD(P)H.
    Photochem Photobiol. 1995 Mar;61(3):261-8 PMID: 7716188
  52. Interactions between the cytosolic components p47phox and p67phox of the human neutrophil NADPH oxidase that are not required for activation in the cell-free system.
    J Biol Chem. 1995 May 12;270(19):11216-21 PMID: 7744754
  53. Electron spin resonance studies on neutrophil cytochrome b558. Evidence that low-spin heme iron is essential for O2-. generating activity.
    J Biol Chem. 1995 May 26;270(21):12685-9 PMID: 7759520
  54. Reconstitution of flavin-depleted neutrophil flavocytochrome b558 with 8-mercapto-FAD and characterization of the flavin-reconstituted enzyme.
    J Biol Chem. 1995 Jul 7;270(27):16428-34 PMID: 7608214
  55. Cytochrome b-245 of the neutrophil superoxide-generating system contains two nonidentical hemes. Potentiometric studies of a mutant form of gp91phox.
    J Biol Chem. 1995 Jul 21;270(29):17075-7 PMID: 7615499
  56. Mapping sites of interaction of p47-phox and flavocytochrome b with random-sequence peptide phage display libraries.
    Proc Natl Acad Sci U S A. 1995 Jul 18;92(15):7110-4 PMID: 7624379
  57. The phosphorylation targets of p47phox, a subunit of the respiratory burst oxidase. Functions of the individual target serines as evaluated by site-directed mutagenesis.
    J Clin Invest. 1995 Sep;96(3):1499-505 PMID: 7657821
  58. Molecular analysis in three cases of X91- variant chronic granulomatous disease.
    Blood. 1995 Nov 1;86(9):3575-82 PMID: 7579466
  59. Stoichiometry of p22-phox and gp91-phox in phagocyte cytochrome b558.
    Biochemistry. 1995 Dec 26;34(51):16753-7 PMID: 8527449
  60. Aerobic and anaerobic functioning of superoxide-producing cytochrome b-559 reconstituted with phospholipids.
    Biochim Biophys Acta. 1995 Dec 12;1232(3):225-9 PMID: 8534675
  61. Mutations in the X-linked and autosomal recessive forms of chronic granulomatous disease.
    Blood. 1996 Mar 1;87(5):1663-81 PMID: 8634410
  62. The FRE1 ferric reductase of Saccharomyces cerevisiae is a cytochrome b similar to that of NADPH oxidase.
    J Biol Chem. 1996 Jun 14;271(24):14240-4 PMID: 8662973
  63. Intracellular pH regulation during spreading of human neutrophils.
    J Cell Biol. 1996 Jun;133(6):1391-402 PMID: 8682873
  64. Assembly and activation of the phagocyte NADPH oxidase. Specific interaction of the N-terminal Src homology 3 domain of p47phox with p22phox is required for activation of the NADPH oxidase.
    J Biol Chem. 1996 Sep 6;271(36):22152-8 PMID: 8703027
  65. Involvement of superoxide and myeloperoxidase in oxygen-dependent killing of Staphylococcus aureus by neutrophils.
    Infect Immun. 1996 Sep;64(9):3512-7 PMID: 8751892
  66. NADPH oxidase activity is independent of p47phox in vitro.
    J Biol Chem. 1996 Sep 13;271(37):22578-82 PMID: 8798426
  67. Increased respiratory burst in myeloperoxidase-deficient monocytes.
    Blood. 1983 Oct;62(4):902-9 PMID: 6309288
  68. NIH conference. Recent advances in chronic granulomatous disease.
    Ann Intern Med. 1983 Nov;99(5):657-74 PMID: 6227266
  69. Complementation in monocyte hybrids revealing genetic heterogeneity in chronic granulomatous disease.
    Nature. 1984 Feb 9-15;307(5951):553-5 PMID: 6320013
  70. Novel domains in NADPH oxidase subunits, sorting nexins, and PtdIns 3-kinases: binding partners of SH3 domains?
    Protein Sci. 1996 Nov;5(11):2353-7 PMID: 8931154
  71. Paxilline inhibition of the alpha-subunit of the high-conductance calcium-activated potassium channel.
    Neuropharmacology. 1996;35(7):963-8 PMID: 8938726
  72. The cytosolic component p47(phox) is not a sine qua non participant in the activation of NADPH oxidase but is required for optimal superoxide production.
    J Biol Chem. 1996 Nov 29;271(48):30326-9 PMID: 8939991
  73. Analysis of activation-induced conformational changes in p47phox using tryptophan fluorescence spectroscopy.
    J Biol Chem. 1997 Nov 21;272(47):29502-10 PMID: 9368011
  74. Essential requirement of cytosolic phospholipase A2 for activation of the phagocyte NADPH oxidase.
    J Biol Chem. 1998 Jan 2;273(1):441-5 PMID: 9417101
  75. Neutrophils exposed to bacterial lipopolysaccharide upregulate NADPH oxidase assembly.
    J Clin Invest. 1998 Jan 15;101(2):455-63 PMID: 9435318
  76. Potassium channels in the vasculature.
    Curr Opin Nephrol Hypertens. 1998 Jan;7(1):91-8 PMID: 9442369
  77. The PC motif: a novel and evolutionarily conserved sequence involved in interaction between p40phox and p67phox, SH3 domain-containing cytosolic factors of the phagocyte NADPH oxidase.
    Eur J Biochem. 1998 Feb 1;251(3):583-9 PMID: 9490029
  78. A plant homolog of the neutrophil NADPH oxidase gp91phox subunit gene encodes a plasma membrane protein with Ca2+ binding motifs.
    Plant Cell. 1998 Feb;10(2):255-66 PMID: 9490748
  79. Activation of human neutrophil nicotinamide adenine dinucleotide phosphate, reduced (triphosphopyridine nucleotide, reduced) oxidase by arachidonic acid in a cell-free system.
    J Clin Invest. 1985 May;75(5):1740-3 PMID: 2987311
  80. Cytochrome b-245 from human neutrophils is a glycoprotein.
    Biochem J. 1985 May 1;227(3):783-8 PMID: 2408606
  81. Production of the superoxide adduct of myeloperoxidase (compound III) by stimulated human neutrophils and its reactivity with hydrogen peroxide and chloride.
    Biochem J. 1985 Jun 15;228(3):583-92 PMID: 2992450
  82. Stimulated neutrophils from patients with autosomal recessive chronic granulomatous disease fail to phosphorylate a Mr-44,000 protein.
    Nature. 1985 Aug 8-14;316(6028):547-9 PMID: 4033752
  83. Chemotactic factor-induced activation of Na+/H+ exchange in human neutrophils. II. Intracellular pH changes.
    J Biol Chem. 1985 Oct 25;260(24):13248-55 PMID: 2997160
  84. Activation of NADPH-dependent superoxide production in a cell-free system by sodium dodecyl sulfate.
    J Biol Chem. 1985 Nov 5;260(25):13539-45 PMID: 2997168
  85. Superoxide production by polymorphonuclear leukocytes. A cytochemical approach.
    Histochemistry. 1986;84(4-6):371-8 PMID: 3013809
  86. Electron spin resonance studies on a flavoprotein in neutrophil plasma membranes. Redox potentials of the flavin and its participation in NADPH oxidase.
    J Biol Chem. 1986 Jul 15;261(20):9426-32 PMID: 3013889
  87. Activation of p47(PHOX), a cytosolic subunit of the leukocyte NADPH oxidase. Phosphorylation of ser-359 or ser-370 precedes phosphorylation at other sites and is required for activity.
    J Biol Chem. 1998 Dec 25;273(52):35147-52 PMID: 9857051
  88. Spontaneous activation of NADPH oxidase in a cell-free system: unexpected multiple effects of magnesium ion concentrations.
    Biochem J. 1999 Feb 15;338 ( Pt 1):229-33 PMID: 9931320
  89. NADPH oxidase: an update.
    Blood. 1999 Mar 1;93(5):1464-76 PMID: 10029572
  90. Guanine nucleotides stimulate NADPH oxidase in membranes of human neutrophils.
    FEBS Lett. 1986 Sep 1;205(1):161-5 PMID: 3017756
  91. The respiratory burst oxidase of human neutrophils. Further studies of the purified enzyme.
    J Biol Chem. 1986 Oct 5;261(28):13247-51 PMID: 3759962
  92. The effect of the inhibitor diphenylene iodonium on the superoxide-generating system of neutrophils. Specific labelling of a component polypeptide of the oxidase.
    Biochem J. 1986 Jul 1;237(1):111-6 PMID: 3800872
  93. Regulation of neutrophil NADPH oxidase activation in a cell-free system by guanine nucleotides and fluoride. Evidence for participation of a pertussis and cholera toxin-insensitive G protein.
    J Biol Chem. 1987 Feb 5;262(4):1685-90 PMID: 3027097
  94. Absence of both cytochrome b-245 subunits from neutrophils in X-linked chronic granulomatous disease.
    Nature. 1987 Mar 5-11;326(6108):88-91 PMID: 3821877
  95. The glycoprotein encoded by the X-linked chronic granulomatous disease locus is a component of the neutrophil cytochrome b complex.
    Nature. 1987 Jun 25-Jul 1;327(6124):717-20 PMID: 3600768
  96. The X-linked chronic granulomatous disease gene codes for the beta-chain of cytochrome b-245.
    Nature. 1987 Jun 25-Jul 1;327(6124):720-1 PMID: 3600769
  97. The superoxide-generating NADPH oxidase of human neutrophils is electrogenic and associated with an H+ channel.
    Biochem J. 1987 Sep 1;246(2):325-9 PMID: 2825632
  98. The quaternary structure of the plasma membrane b-type cytochrome of human granulocytes.
    Biochim Biophys Acta. 1988 Jan 20;932(1):71-83 PMID: 3337799
  99. Chronic granulomatous disease due to a defect in the cytosolic factor required for nicotinamide adenine dinucleotide phosphate oxidase activation.
    J Clin Invest. 1988 Feb;81(2):606-10 PMID: 3339133
  100. Myeloperoxidase deficiency.
    Hematol Oncol Clin North Am. 1988 Mar;2(1):135-58 PMID: 2831185
  101. The superoxide-forming enzymatic system of phagocytes.
    Free Radic Biol Med. 1988;4(4):225-61 PMID: 2834275
  102. Primary structure and unique expression of the 22-kilodalton light chain of human neutrophil cytochrome b.
    Proc Natl Acad Sci U S A. 1988 May;85(10):3319-23 PMID: 3368442
  103. Activation of bovine neutrophil oxidase in a cell free system. GTP-dependent formation of a complex between a cytosolic factor and a membrane protein.
    Biochem Biophys Res Commun. 1988 May 16;152(3):993-1001 PMID: 3132160
  104. Phagocyte NADPH-oxidase. Studies with flavin analogues as active site probes in triton X-100-solubilized preparations.
    J Biol Chem. 1988 Jun 25;263(18):8859-63 PMID: 3379049
  105. Internal pH changes associated with the activity of NADPH oxidase of human neutrophils. Further evidence for the presence of an H+ conducting channel.
    Biochem J. 1988 Apr 15;251(2):563-7 PMID: 2456757
  106. Phosphorylation of the subunits of cytochrome b-245 upon triggering of the respiratory burst of human neutrophils and macrophages.
    Biochem J. 1988 Jun 15;252(3):901-4 PMID: 2844158
  107. Proton release associated with respiratory burst of polymorphonuclear leukocytes.
    J Biochem. 1988 Apr;103(4):656-60 PMID: 2844739
  108. Two cytosolic neutrophil oxidase components absent in autosomal chronic granulomatous disease.
    Science. 1988 Dec 2;242(4883):1295-7 PMID: 2848318
  109. Superoxide generation by the electrogenic NADPH oxidase of human neutrophils is limited by the movement of a compensating charge.
    Biochem J. 1988 Oct 1;255(1):285-90 PMID: 2848506
  110. Purification and some properties of the small subunit of cytochrome b558 from human neutrophils.
    J Biol Chem. 1989 Jan 5;264(1):112-8 PMID: 2909509
  111. Cytosolic components of the respiratory burst oxidase: resolution of four components, two of which are missing in complementing types of chronic granulomatous disease.
    Proc Natl Acad Sci U S A. 1989 Feb;86(3):825-9 PMID: 2915980
  112. The plasma membrane potential of human neutrophils. Role of ion channels and the sodium/potassium pump.
    Biochim Biophys Acta. 1989 Apr 14;980(2):139-45 PMID: 2539191
  113. Functional defect in neutrophil cytosols from two patients with autosomal recessive cytochrome-positive chronic granulomatous disease.
    J Clin Invest. 1989 Apr;83(4):1236-40 PMID: 2539395
  114. Absence of both the 91kD and 22kD subunits of human neutrophil cytochrome b in two genetic forms of chronic granulomatous disease.
    Blood. 1989 May 1;73(6):1416-20 PMID: 2713485
  115. Respiratory burst oxidase and three of four oxidase-related polypeptides are associated with the cytoskeleton of human neutrophils.
    J Clin Invest. 1991 Apr;87(4):1345-51 PMID: 1849148
  116. Characterization of neutrophil b-type cytochrome in situ by electron paramagnetic resonance spectroscopy.
    FEBS Lett. 1991 Apr 9;281(1-2):130-2 PMID: 1849835
  117. Identification of a superoxide-generating NADPH oxidase system in human fibroblasts.
    Biochem J. 1991 Apr 1;275 ( Pt 1):241-5 PMID: 1850240
  118. Application of spin trapping to human phagocytic cells: insight into conditions for formation and limitation of hydroxyl radical.
    Free Radic Res Commun. 1991;12-13 Pt 1:17-25 PMID: 1649085
  119. Activation of the NADPH oxidase involves the small GTP-binding protein p21rac1.
    Nature. 1991 Oct 17;353(6345):668-70 PMID: 1922386
  120. Protein kinase C activates an H+ (equivalent) conductance in the plasma membrane of human neutrophils.
    Proc Natl Acad Sci U S A. 1991 Dec 1;88(23):10816-20 PMID: 1720552
  121. Regulation of phagocyte oxygen radical production by the GTP-binding protein Rac 2.
    Science. 1991 Dec 6;254(5037):1512-5 PMID: 1660188
  122. Purification and characterization of a third cytosolic component of the superoxide-generating NADPH oxidase of macrophages.
    J Biol Chem. 1991 Dec 15;266(35):23577-85 PMID: 1660877
  123. Phorbol 12-myristate 13-acetate activates an electrogenic H(+)-conducting pathway in the membrane of neutrophils.
    Biochem J. 1992 Feb 1;281 ( Pt 3):697-701 PMID: 1371386
  124. Cytochrome b-245 is a flavocytochrome containing FAD and the NADPH-binding site of the microbicidal oxidase of phagocytes.
    Biochem J. 1992 Jun 15;284 ( Pt 3):781-8 PMID: 1320378
  125. Separation of human neutrophil plasma membrane from intracellular vesicles containing alkaline phosphatase and NADPH oxidase activity by free flow electrophoresis.
    J Biol Chem. 1992 Jul 25;267(21):14912-7 PMID: 1634531
  126. EPR signals of cytochrome b558 purified from porcine neutrophils.
    J Biol Chem. 1992 Sep 25;267(27):19673-5 PMID: 1326560
  127. Activation of vacuolar-type proton pumps by protein kinase C. Role in neutrophil pH regulation.
    J Biol Chem. 1992 Nov 15;267(32):22740-6 PMID: 1331065
  128. Chronic granulomatous disease with partial deficiency of cytochrome b558 and incomplete respiratory burst: variants of the X-linked, cytochrome b558-negative form of the disease.
    J Leukoc Biol. 1992 Feb;51(2):164-71 PMID: 1431553
  129. The cytochrome b-558 molecules involved in the fibroblast and polymorphonuclear leucocyte superoxide-generating NADPH oxidase systems are structurally and genetically distinct.
    Biochem J. 1993 Jan 15;289 ( Pt 2):481-6 PMID: 7678734
  130. V-ATPases in phagocytic cells.
    J Exp Biol. 1992 Nov;172:179-92 PMID: 1491224
  131. Neutrophil nicotinamide adenine dinucleotide phosphate oxidase assembly. Translocation of p47-phox and p67-phox requires interaction between p47-phox and cytochrome b558.
    J Clin Invest. 1991 Jan;87(1):352-6 PMID: 1985107
  132. Resonance Raman and electron paramagnetic resonance structural investigations of neutrophil cytochrome b558.
    J Biol Chem. 1991 Jan 25;266(3):1627-34 PMID: 1846361
  133. Iodination by stimulated human neutrophils. Studies on its stoichiometry, subcellular localization and relevance to microbial killing.
    Biochem J. 1983 Jan 15;210(1):215-25 PMID: 6303312
  134. Critical assessment of the presence of an NADPH binding site on neutrophil cytochrome b558 by photoaffinity and immunochemical labeling.
    Biochemistry. 1993 Aug 31;32(34):8880-7 PMID: 8364034
  135. Purification of the 260 kDa cytosolic complex involved in the superoxide production of guinea pig neutrophils.
    FEBS Lett. 1993 Sep 13;330(2):215-8 PMID: 8396047
  136. Role of the rac1 p21-GDP-dissociation inhibitor for rho heterodimer in the activation of the superoxide-forming NADPH oxidase of macrophages.
    Eur J Biochem. 1993 Oct 1;217(1):441-55 PMID: 8223583
  137. Affinity-labeling of an NADPH-binding site on the heavy subunit of flavocytochrome b558 in particulate NADPH oxidase from activated human neutrophils.
    Biochem Biophys Res Commun. 1993 Oct 29;196(2):543-52 PMID: 8240326
  138. A structural model for the nucleotide binding domains of the flavocytochrome b-245 beta-chain.
    Protein Sci. 1993 Oct;2(10):1675-85 PMID: 8251942
  139. Activation of NADPH oxidase of human neutrophils involves the phosphorylation and the translocation of cytosolic p67phox.
    Biochem J. 1993 Dec 1;296 ( Pt 2):367-71 PMID: 8257426
  140. Potential, pH, and arachidonate gate hydrogen ion currents in human neutrophils.
    Biophys J. 1993 Oct;65(4):1590-8 PMID: 7506066
  141. p40phox, a third cytosolic component of the activation complex of the NADPH oxidase to contain src homology 3 domains.
    Biochem J. 1993 Dec 15;296 ( Pt 3):557-61 PMID: 8280052
  142. Superoxide production by cytochrome b559. Mechanism of cytosol-independent activation.
    FEBS Lett. 1994 Feb 7;338(3):285-9 PMID: 8307196
  143. Complementation of NADPH oxidase in p67-phox-deficient CGD patients p67-phox/p40-phox interaction.
    Eur J Biochem. 2000 Feb;267(4):1059-67 PMID: 10672014
  144. Impaired immunity and enhanced resistance to endotoxin in the absence of neutrophil elastase and cathepsin G.
    Immunity. 2000 Feb;12(2):201-10 PMID: 10714686
  145. NAD(P)H oxidase: role in cardiovascular biology and disease.
    Circ Res. 2000 Mar 17;86(5):494-501 PMID: 10720409
  146. Potassium channel openers as potential therapeutic weapons in ion channel disease.
    Kidney Int. 2000 Mar;57(3):838-45 PMID: 10720937
  147. X-ray crystal structure and characterization of halide-binding sites of human myeloperoxidase at 1.8 A resolution.
    J Biol Chem. 2000 Apr 21;275(16):11964-71 PMID: 10766826
  148. Human neutrophil immunodeficiency syndrome is associated with an inhibitory Rac2 mutation.
    Proc Natl Acad Sci U S A. 2000 Apr 25;97(9):4654-9 PMID: 10758162
  149. Arachidonic acid and phosphorylation synergistically induce a conformational change of p47phox to activate the phagocyte NADPH oxidase.
    J Biol Chem. 2000 May 5;275(18):13793-801 PMID: 10788501
  150. Processing and maturation of flavocytochrome b558 include incorporation of heme as a prerequisite for heterodimer assembly.
    J Biol Chem. 2000 May 5;275(18):13986-93 PMID: 10788525
  151. 156Pro-->Gln substitution in the light chain of cytochrome b558 of the human NADPH oxidase (p22-phox) leads to defective translocation of the cytosolic proteins p47-phox and p67-phox.
    J Exp Med. 1994 Dec 1;180(6):2329-34 PMID: 7964505
  152. Rac translocates independently of the neutrophil NADPH oxidase components p47phox and p67phox. Evidence for its interaction with flavocytochrome b558.
    J Biol Chem. 1994 Dec 9;269(49):30749-52 PMID: 7982999
  153. Methoxatin (PQQ) in guinea-pig neutrophils.
    Free Radic Biol Med. 1994 Oct;17(4):311-20 PMID: 8001835
  154. P67-phox-mediated NADPH oxidase assembly: imaging of cytochrome b558 liposomes by atomic force microscopy.
    Biochemistry. 2000 Aug 8;39(31):9302-10 PMID: 10924123
  155. Cloning of two human thyroid cDNAs encoding new members of the NADPH oxidase family.
    J Biol Chem. 2000 Jul 28;275(30):23227-33 PMID: 10806195
  156. Dominant negative mutation of the hematopoietic-specific Rho GTPase, Rac2, is associated with a human phagocyte immunodeficiency.
    Blood. 2000 Sep 1;96(5):1646-54 PMID: 10961859
  157. Phosphorylation of p22phox is mediated by phospholipase D-dependent and -independent mechanisms. Correlation of NADPH oxidase activity and p22phox phosphorylation.
    J Biol Chem. 2000 Sep 15;275(37):28406-12 PMID: 10893420
  158. Role of reactive oxygen intermediates and cognate redox signaling in disease resistance.
    Plant Physiol. 2000 Sep;124(1):21-9 PMID: 10982418
  159. Direct interaction of actin with p47(phox) of neutrophil NADPH oxidase.
    Biochem Biophys Res Commun. 2000 Oct 5;276(3):1186-90 PMID: 11027608
  160. Hematologically important mutations: the autosomal recessive forms of chronic granulomatous disease (first update).
    Blood Cells Mol Dis. 2000 Oct;26(5):561-5 PMID: 11112388
  161. Targeting of Rac1 to the phagocyte membrane is sufficient for the induction of NADPH oxidase assembly.
    J Biol Chem. 2000 Dec 22;275(51):40073-81 PMID: 11007780
  162. Twenty-first century phox: not yet ready for widespread screening.
    Circulation. 2001 Jan 2;103(1):7-9 PMID: 11136676
  163. p40(phox) Participates in the activation of NADPH oxidase by increasing the affinity of p47(phox) for flavocytochrome b(558).
    Biochem J. 2000 Jul 1;349(Pt 1):113-7 PMID: 10861218
  164. Hematologically important mutations: X-linked chronic granulomatous disease (second update).
    Blood Cells Mol Dis. 2001 Jan-Feb;27(1):16-26 PMID: 11162142
  165. Roles for beta II-protein kinase C and RACK1 in positive and negative signaling for superoxide anion generation in differentiated HL60 cells.
    J Biol Chem. 2001 Mar 23;276(12):8910-7 PMID: 11120743
  166. Superoxide excess in hypertension and aging: a common cause of endothelial dysfunction.
    Hypertension. 2001 Feb;37(2 Pt 2):529-34 PMID: 11230330
  167. Molecular basis for Rac2 regulation of phagocyte NADPH oxidase.
    Nat Immunol. 2001 Mar;2(3):211-5 PMID: 11224519
  168. Biochemical and biological characterization of a human Rac2 GTPase mutant associated with phagocytic immunodeficiency.
    J Biol Chem. 2001 May 11;276(19):15929-38 PMID: 11278678
  169. Solution structure of the PX domain, a target of the SH3 domain.
    Nat Struct Biol. 2001 Jun;8(6):526-30 PMID: 11373621
  170. The PX domains of p47phox and p40phox bind to lipid products of PI(3)K.
    Nat Cell Biol. 2001 Jul;3(7):675-8 PMID: 11433300
  171. PtdIns(3)P regulates the neutrophil oxidase complex by binding to the PX domain of p40(phox).
    Nat Cell Biol. 2001 Jul;3(7):679-82 PMID: 11433301
  172. Expression of reduced nicotinamide adenine dinucleotide phosphate oxidase (ThoX, LNOX, Duox) genes and proteins in human thyroid tissues.
    J Clin Endocrinol Metab. 2001 Jul;86(7):3351-8 PMID: 11443211
  173. Novel modular domain PB1 recognizes PC motif to mediate functional protein-protein interactions.
    EMBO J. 2001 Aug 1;20(15):3938-46 PMID: 11483497
  174. Heme histidine ligands within gp91(phox) modulate proton conduction by the phagocyte NADPH oxidase.
    J Biol Chem. 2001 Aug 10;276(32):30277-84 PMID: 11389135
  175. Heme-ligating histidines in flavocytochrome b(558): identification of specific histidines in gp91(phox).
    J Biol Chem. 2001 Aug 17;276(33):31105-12 PMID: 11413138
  176. Mechanism of NADPH oxidase activation by the Rac/Rho-GDI complex.
    Biochemistry. 2001 Aug 28;40(34):10014-22 PMID: 11513579
  177. A kinetic analysis of the catalase activity of myeloperoxidase.
    Biochemistry. 2001 Aug 28;40(34):10204-12 PMID: 11513598
  178. Tyrosine cross-linking of extracellular matrix is catalyzed by Duox, a multidomain oxidase/peroxidase with homology to the phagocyte oxidase subunit gp91phox.
    J Cell Biol. 2001 Aug 20;154(4):879-91 PMID: 11514595
  179. Intramembrane bis-heme motif for transmembrane electron transport conserved in a yeast iron reductase and the human NADPH oxidase.
    J Biol Chem. 1996 Dec 6;271(49):31021-4 PMID: 8940093
  180. Stoichiometry of the subunits of flavocytochrome b558 of the NADPH oxidase of phagocytes.
    Biochem J. 1996 Nov 15;320 ( Pt 1):33-8 PMID: 8947464
  181. Assembly of the phagocyte NADPH oxidase: molecular interaction of oxidase proteins.
    J Leukoc Biol. 1996 Dec;60(6):677-91 PMID: 8975869
  182. Analysis of glycosylation sites on gp91phox, the flavocytochrome of the NADPH oxidase, by site-directed mutagenesis and translation in vitro.
    Biochem J. 1997 Feb 1;321 ( Pt 3):583-5 PMID: 9032440
  183. Involvement of p40phox in activation of phagocyte NADPH oxidase through association of its carboxyl-terminal, but not its amino-terminal, with p67phox.
    J Exp Med. 1996 Sep 1;184(3):893-902 PMID: 9064349
  184. Cloned potassium channels from eukaryotes and prokaryotes.
    Annu Rev Neurosci. 1997;20:91-123 PMID: 9056709
  185. p40(phox) down-regulates NADPH oxidase activity through interactions with its SH3 domain.
    J Biol Chem. 1997 Apr 4;272(14):9141-6 PMID: 9083043
  186. An in-frame triplet deletion within the gp91-phox gene in an adult X-linked chronic granulomatous disease patient with residual NADPH-oxidase activity.
    Eur J Haematol. 1997 Feb;58(2):78-85 PMID: 9111587
  187. Electron transfer in the superoxide-generating NADPH oxidase complex reconstituted in vitro.
    Biochim Biophys Acta. 1997 Apr 11;1319(2-3):139-46 PMID: 9131041
  188. A modulator of rho family G proteins, rhoGDI, binds these G proteins via an immunoglobulin-like domain and a flexible N-terminal arm.
    Structure. 1997 May 15;5(5):623-33 PMID: 9195882
  189. Activation of the leukocyte NADPH oxidase subunit p47phox by protein kinase C. A phosphorylation-dependent change in the conformation of the C-terminal end of p47phox.
    Biochemistry. 1997 Jun 17;36(24):7474-80 PMID: 9200696
  190. Crystal structure of the cytochrome bc1 complex from bovine heart mitochondria.
    Science. 1997 Jul 4;277(5322):60-6 PMID: 9204897
  191. Biosynthesis of the phagocyte NADPH oxidase cytochrome b558. Role of heme incorporation and heterodimer formation in maturation and stability of gp91phox and p22phox subunits.
    J Biol Chem. 1997 Oct 24;272(43):27288-94 PMID: 9341176
  192. The membrane-associated component of the amphiphile-activated, cytosol-dependent superoxide-forming NADPH oxidase of macrophages is identical to cytochrome b559.
    J Biol Chem. 1991 Feb 15;266(5):2795-804 PMID: 1847135
  193. Subcellular localization of the b-cytochrome component of the human neutrophil microbicidal oxidase: translocation during activation.
    J Cell Biol. 1983 Jul;97(1):52-61 PMID: 6408102
  194. The cytochrome b and flavin content and properties of the O2- -forming NADPH oxidase solubilized from activated neutrophils.
    Biochim Biophys Acta. 1983 Jul 28;746(1-2):40-7 PMID: 6871231
  195. P-Rex1, a PtdIns(3,4,5)P3- and Gbetagamma-regulated guanine-nucleotide exchange factor for Rac.
    Cell. 2002 Mar 22;108(6):809-21 PMID: 11955434
  196. Catalase negative Staphylococcus aureus retain virulence in mouse model of chronic granulomatous disease.
    FEBS Lett. 2002 May 8;518(1-3):107-10 PMID: 11997027
  197. Hydrogen peroxide and nitric oxide as signalling molecules in plants.
    J Exp Bot. 2002 May;53(372):1237-47 PMID: 11997372
  198. The subcellular localization of ubiquinone in human neutrophils.
    Biochem J. 1983 Dec 15;216(3):765-8 PMID: 6320799
  199. Purification of cytochrome b-245 from human neutrophils.
    Biochem J. 1984 Apr 15;219(2):519-27 PMID: 6331390
  200. Cytochrome b, flavins, and ubiquinone-50 in enucleated human neutrophils (polymorphonuclear leukocyte cytoplasts).
    J Biol Chem. 1984 Aug 10;259(15):9603-6 PMID: 6746662
  201. Proton secretion by stimulated neutrophils. Significance of hexose monophosphate shunt activity as source of electrons and protons for the respiratory burst.
    J Clin Invest. 1984 Aug;74(2):455-9 PMID: 6430961
  202. Unsaturated fatty acids stimulate NADPH-dependent superoxide production by cell-free system derived from macrophages.
    Cell Immunol. 1984 Oct 1;88(1):213-21 PMID: 6090027
  203. Activation of a NADPH oxidase from horse polymorphonuclear leukocytes in a cell-free system.
    J Leukoc Biol. 1984 Dec;36(6):751-9 PMID: 6594417
  204. Activation of the human neutrophil NADPH oxidase results in coupling of electron carrier function between ubiquinone-10 and cytochrome b559.
    J Biol Chem. 1985 Apr 10;260(7):3991-5 PMID: 2984192
  205. Mechanism of the superoxide-producing oxidase of neutrophils. O2 is necessary for the fast reduction of cytochrome b-245 by NADPH.
    Biochem J. 1985 Mar 15;226(3):881-4 PMID: 2985050
  206. Activation of the respiratory burst enzyme from human neutrophils in a cell-free system. Evidence for a soluble cofactor.
    J Clin Invest. 1985 May;75(5):1735-9 PMID: 2987310
  207. PX domain takes shape.
    Curr Opin Hematol. 2003 Jan;10(1):2-7 PMID: 12483105
  208. Interactions of oxidants with vascular signaling systems.
    Arterioscler Thromb Vasc Biol. 2000 Jun;20(6):1430-42 PMID: 10845855
  209. Two novel proteins activate superoxide generation by the NADPH oxidase NOX1.
    J Biol Chem. 2003 Feb 7;278(6):3510-3 PMID: 12473664
  210. Reactive oxygen species produced by NADPH oxidase regulate plant cell growth.
    Nature. 2003 Mar 27;422(6930):442-6 PMID: 12660786
  211. The voltage dependence of NADPH oxidase reveals why phagocytes need proton channels.
    Nature. 2003 Apr 3;422(6931):531-4 PMID: 12673252
  212. Molecular basis of phosphorylation-induced activation of the NADPH oxidase.
    Cell. 2003 May 2;113(3):343-55 PMID: 12732142
  213. Proteins homologous to p47phox and p67phox support superoxide production by NAD(P)H oxidase 1 in colon epithelial cells.
    J Biol Chem. 2003 May 30;278(22):20006-12 PMID: 12657628
  214. Reassessment of the microbicidal activity of reactive oxygen species and hypochlorous acid with reference to the phagocytic vacuole of the neutrophil granulocyte.
    J Med Microbiol. 2003 Aug;52(Pt 8):643-51 PMID: 12867557
  215. Cloning the gene for an inherited human disorder--chronic granulomatous disease--on the basis of its chromosomal location.
    Nature. 1986 Jul 3-9;322(6074):32-8 PMID: 2425263
  216. Cytoplasmic pH regulation in phorbol ester-activated human neutrophils.
    Am J Physiol. 1986 Jul;251(1 Pt 1):C55-65 PMID: 2425631
  217. Antimicrobial properties of hydrogen peroxide and sodium bicarbonate individually and in combination against selected oral, gram-negative, facultative bacteria.
    J Dent Res. 1986 Sep;65(9):1142-8 PMID: 3016051
  218. [Electron microscopic and biochemical study of the neutrophilic granules from leucocytes].
    J Electron Microsc (Tokyo). 1966;15(2):81-5 PMID: 5965755
  219. The role of the phagocyte in host-parasite interactions. VII. Di- and triphosphopyridine nucleotide kinetics during phagocytosis.
    Biochim Biophys Acta. 1967 Jul 25;141(2):243-9 PMID: 4382998
  220. Iodination of bacteria: a bactericidal mechanism.
    J Exp Med. 1967 Dec 1;126(6):1063-78 PMID: 4964565
  221. Myeloperoxidase-halide-hydrogen peroxide antibacterial system.
    J Bacteriol. 1968 Jun;95(6):2131-8 PMID: 4970226
  222. Defective bactericidal activity in myeloperoxidase-deficient human neutrophils.
    Nature. 1969 Jul 5;223(5201):78-9 PMID: 4978299
  223. Superoxide dismutase. An enzymic function for erythrocuprein (hemocuprein).
    J Biol Chem. 1969 Nov 25;244(22):6049-55 PMID: 5389100
  224. Intraleukocytic microbicidal defects.
    Annu Rev Med. 1971;22:39-62 PMID: 4400133
  225. Laboratory models of chronic granulomatous disease.
    J Reticuloendothel Soc. 1972 Aug;12(2):216-37 PMID: 4263057
  226. Biological defense mechanisms. The production by leukocytes of superoxide, a potential bactericidal agent.
    J Clin Invest. 1973 Mar;52(3):741-4 PMID: 4346473
  227. Role of peroxide in phagocytic killing of pneumococci.
    Infect Immun. 1974 Jan;9(1):48-52 PMID: 4148725
  228. Biological defense mechanisms. Evidence for the participation of superoxide in bacterial killing by xanthine oxidase.
    J Lab Clin Med. 1975 Feb;85(2):235-44 PMID: 1089740
  229. Catalase, superoxide dismutase, and virulence of Staphylococcus aureus. In vitro and in vivo studies with emphasis on staphylococcal--leukocyte interaction.
    J Clin Invest. 1975 Mar;55(3):561-6 PMID: 1117067
  230. Antimicrobial mechanisms in neutrophilic polymorphonuclear leukocytes.
    Semin Hematol. 1975 Apr;12(2):117-42 PMID: 1118738
  231. Chicken neutrophils: oxidative metabolism in phagocytic cells devoid of myeloperoxidase.
    Proc Natl Acad Sci U S A. 1975 Dec;72(12):5012-5 PMID: 174090
  232. Iodination by human polymorphonuclear leukocytes: a re-evaluation.
    J Lab Clin Med. 1977 Mar;89(3):675-86 PMID: 839126
  233. Absence of a newly described cytochrome b from neutrophils of patients with chronic granulomatous disease.
    Lancet. 1978 Aug 26;2(8087):446-9 PMID: 79807
  234. Fluorescence probe measurement of the intralysosomal pH in living cells and the perturbation of pH by various agents.
    Proc Natl Acad Sci U S A. 1978 Jul;75(7):3327-31 PMID: 28524
  235. Analytical subcellular fractionation of neutrophils from patients with chronic granulomatous disease. Demonstration of the enzyme defect in four cases.
    Q J Med. 1978 Apr;47(186):213-20 PMID: 684156
  236. Solubilization of the O2(-)-forming activity responsible for the respiratory burst in human neutrophils.
    J Biol Chem. 1978 Oct 10;253(19):6663-5 PMID: 690118
  237. Tracing the fate of oxygen consumed during phagocytosis by human neutrophils with 15O2.
    Clin Sci Mol Med. 1978 Oct;55(4):413-5 PMID: 710056
  238. Kinetics of oxygen consumption by phagocytosing human neutrophils.
    Biochem Biophys Res Commun. 1978 Oct 16;84(3):611-7 PMID: 718704
  239. Novel cytochrome b system in phagocytic vacuoles of human granulocytes.
    Nature. 1978 Nov 30;276(5687):515-7 PMID: 723935
  240. Bactericidal activity of a superoxide anion-generating system. A model for the polymorphonuclear leukocyte.
    J Exp Med. 1979 Jan 1;149(1):27-39 PMID: 216766
  241. The enzyme of granulocytes that produces superoxide and peroxide. An elusive Pimpernel.
    N Engl J Med. 1979 May 17;300(20):1157-60 PMID: 219342
  242. Production of superoxide by neutrophils: a reappraisal.
    FEBS Lett. 1979 Apr 1;100(1):27-32 PMID: 220088
  243. Role of arginine-38 in regulation of the cytochrome c oxidation-reduction equilibrium.
    Biochemistry. 1989 Apr 18;28(8):3188-97 PMID: 2545252
  244. Localization of the 47 kDa phosphoprotein involved in the respiratory-burst NADPH oxidase of phagocytic cells.
    Biochem J. 1989 May 15;260(1):243-8 PMID: 2775188
  245. Cloning of the cDNA and functional expression of the 47-kilodalton cytosolic component of human neutrophil respiratory burst oxidase.
    Proc Natl Acad Sci U S A. 1989 Sep;86(18):7195-9 PMID: 2550933
  246. Studies on the electron-transfer mechanism of the human neutrophil NADPH oxidase.
    Biochem J. 1989 Sep 1;262(2):575-9 PMID: 2553003
  247. Indications to an NADPH oxidase as a possible pO2 sensor in the rat carotid body.
    FEBS Lett. 1989 Oct 9;256(1-2):75-8 PMID: 2806553
  248. Asparagine-linked glycosylation of cytochrome b558 large subunit varies in different human phagocytic cells.
    J Immunol. 1989 Dec 15;143(12):4152-7 PMID: 2556477
  249. Activation of the superoxide-forming NADPH oxidase of macrophages requires two cytosolic components--one of them is also present in certain nonphagocytic cells.
    J Immunol. 1989 Dec 15;143(12):4180-7 PMID: 2556480
  250. Neutrophil degranulation inhibits potential hydroxyl-radical formation. Relative impact of myeloperoxidase and lactoferrin release on hydroxyl-radical production by iron-supplemented neutrophils assessed by spin-trapping techniques.
    Biochem J. 1989 Dec 1;264(2):447-55 PMID: 2557840
  251. Voltage-dependent and Ca2(+)-activated ion channels in human neutrophils.
    J Clin Invest. 1990 Feb;85(2):491-8 PMID: 2153708
  252. Inhibitors of the leukocyte superoxide generating oxidase: mechanisms of action and methods for their elucidation.
    Free Radic Biol Med. 1990;8(1):71-93 PMID: 2157636
  253. Cloning of a 67-kD neutrophil oxidase factor with similarity to a noncatalytic region of p60c-src.
    Science. 1990 May 11;248(4956):727-30 PMID: 1692159
  254. Metabolic activation of natural phenols into selective oxidative burst agonists by activated human neutrophils.
    Free Radic Biol Med. 1990;8(3):251-8 PMID: 2160411
  255. Evidence for a functional cytoplasmic domain of phagocyte oxidase cytochrome b558.
    J Biol Chem. 1990 May 25;265(15):8745-50 PMID: 2160466
  256. Purification and characterization of a unique, potent, peptidyl probe for the high conductance calcium-activated potassium channel from venom of the scorpion Buthus tamulus.
    J Biol Chem. 1990 Jul 5;265(19):11083-90 PMID: 1694175
  257. Human neutrophil cytochrome b light chain (p22-phox). Gene structure, chromosomal location, and mutations in cytochrome-negative autosomal recessive chronic granulomatous disease.
    J Clin Invest. 1990 Nov;86(5):1729-37 PMID: 2243141
  258. Proteoglycans in haemopoietic cells.
    Biochim Biophys Acta. 1990 Dec 11;1032(2-3):191-211 PMID: 2261494
  259. A prenylated p67phox-Rac1 chimera elicits NADPH-dependent superoxide production by phagocyte membranes in the absence of an activator and of p47phox: conversion of a pagan NADPH oxidase to monotheism.
    J Biol Chem. 2002 May 24;277(21):18605-10 PMID: 11896062
  260. Absence of proton channels in COS-7 cells expressing functional NADPH oxidase components.
    J Gen Physiol. 2002 Jun;119(6):571-80 PMID: 12034764
  261. Inactivating mutations in the gene for thyroid oxidase 2 (THOX2) and congenital hypothyroidism.
    N Engl J Med. 2002 Jul 11;347(2):95-102 PMID: 12110737
  262. Voltage-gated H+ channels associated with human phagocyte superoxide-generating NADPH oxidases: sequence comparisons, structural predictions, and phylogenetic analyses.
    Mol Membr Biol. 2002 Apr-Jun;19(2):137-47 PMID: 12126231
  263. Mutagenesis of p22(phox) histidine 94. A histidine in this position is not required for flavocytochrome b558 function.
    J Biol Chem. 2002 Aug 16;277(33):30368-74 PMID: 12042318
  264. Voltage-gated proton "channels": a spectator's viewpoint.
    J Gen Physiol. 2002 Dec;120(6):767-71 PMID: 12451046
  265. The gp91phox component of NADPH oxidase is not a voltage-gated proton channel.
    J Gen Physiol. 2002 Dec;120(6):773-9 PMID: 12451047
  266. The adaptor protein p40(phox) as a positive regulator of the superoxide-producing phagocyte oxidase.
    EMBO J. 2002 Dec 2;21(23):6312-20 PMID: 12456638
  267. Evidence for antibody-catalyzed ozone formation in bacterial killing and inflammation.
    Science. 2002 Dec 13;298(5601):2195-9 PMID: 12434011
  268. Natural engineering principles of electron tunnelling in biological oxidation-reduction.
    Nature. 1999 Nov 4;402(6757):47-52 PMID: 10573417
  269. Phosphorylation of p40-phox during activation of neutrophil NADPH oxidase.
    J Leukoc Biol. 1999 Nov;66(5):851-7 PMID: 10577519
  270. Evidence that the product of the human X-linked CGD gene, gp91-phox, is a voltage-gated H(+) pathway.
    J Gen Physiol. 1999 Dec;114(6):771-86 PMID: 10578014
  271. Kinetic study of the activation of the neutrophil NADPH oxidase by arachidonic acid. Antagonistic effects of arachidonic acid and phenylarsine oxide.
    Biochemistry. 1999 Dec 7;38(49):16394-406 PMID: 10587465
  272. Purification of a novel flavoprotein involved in the thyroid NADPH oxidase. Cloning of the porcine and human cdnas.
    J Biol Chem. 1999 Dec 24;274(52):37265-9 PMID: 10601291
  273. Identification of a cytochrome b-type NAD(P)H oxidoreductase ubiquitously expressed in human cells.
    Proc Natl Acad Sci U S A. 1999 Dec 21;96(26):14742-7 PMID: 10611283
  274. A mammalian H+ channel generated through alternative splicing of the NADPH oxidase homolog NOH-1.
    Science. 2000 Jan 7;287(5450):138-42 PMID: 10615049
  275. Central role for G protein-coupled phosphoinositide 3-kinase gamma in inflammation.
    Science. 2000 Feb 11;287(5455):1049-53 PMID: 10669418
  276. Kinase-dependent change in the conformation of the leukocyte NADPH oxidase subunit p47phox.
    Mol Cells. 1999 Dec 31;9(6):652-6 PMID: 10672933
  277. The large-conductance Ca2+-activated K+ channel is essential for innate immunity.
    Nature. 2004 Feb 26;427(6977):853-8 PMID: 14985765
  278. A new cytochrome in neurophilic granules of rabbit leucocyte.
    J Biochem. 1966 Jun;59(6):622-4 PMID: 4289945
  279. Role of the vascular NADH/NADPH oxidase system in atherosclerosis.
    Ann N Y Acad Sci. 2000 May;902:241-7; discussion 247-8 PMID: 10865844
  280. Vascular gp91(phox): beyond the endothelium.
    Circ Res. 2000 Jul 7;87(1):1-3 PMID: 10884362
  281. Identification of renox, an NAD(P)H oxidase in kidney.
    Proc Natl Acad Sci U S A. 2000 Jul 5;97(14):8010-4 PMID: 10869423
  282. Cytochrome b is present in neutrophils from patients with chronic granulomatous disease.
    Lancet. 1979 May 5;1(8123):949-51 PMID: 87617
  283. The subcellular distribution and some properties of the cytochrome b component of the microbicidal oxidase system of human neutrophils.
    Biochem J. 1979 Jul 15;182(1):181-8 PMID: 496906
  284. Structural and catalytic properties of the solubilized superoxide-generating activity of human polymorphonuclear leukocytes. Solubilization, stabilization in solution, and partial characterization.
    Biochemistry. 1979 Dec 11;18(25):5576-84 PMID: 229897
  285. Absence of cytochrome b reduction in stimulated neutrophils from both female and male patients with chronic granulomatous disease.
    FEBS Lett. 1980 Jan 28;110(1):111-4 PMID: 7353656
  286. Rapid incorporation of the human neutrophil plasma membrane cytochrome b into phagocytic vacuoles.
    Biochem Biophys Res Commun. 1980 Jan 29;92(2):710-5 PMID: 7356490
  287. Lactoferrin enhances hydroxyl radical production by human neutrophils, neutrophil particulate fractions, and an enzymatic generating system.
    J Clin Invest. 1981 Feb;67(2):352-60 PMID: 6780607
  288. The respiratory burst of phagocytic cells is associated with a rise in vacuolar pH.
    Nature. 1981 Apr 2;290(5805):406-9 PMID: 7219526
  289. Role of hydroxyl radical in polymorphonuclear leukocyte-mediated bactericidal activity.
    Agents Actions Suppl. 1980;7:180-4 PMID: 6264770
  290. Extracellular proton release by stimulated neutrophils.
    J Clin Invest. 1981 Jul;68(1):310-3 PMID: 6265500
  291. Evidence that a quinone may be required for the production of superoxide and hydrogen peroxide in neutrophils.
    Biochem Biophys Res Commun. 1981 Apr 30;99(4):1277-86 PMID: 6266419
  292. A variant of chronic granulomatous disease: deficient oxidative metabolism due to a low-affinity NADPH oxidase.
    N Engl J Med. 1981 Nov 26;305(22):1329-33 PMID: 6270561
  293. Oxidation-reduction properties of the cytochrome b found in the plasma-membrane fraction of human neutrophils. A possible oxidase in the respiratory burst.
    Biochem J. 1981 Feb 15;194(2):599-606 PMID: 7306004
  294. Cytochrome b-245 of neutrophils is also present in human monocytes, macrophages and eosinophils.
    Biochem J. 1981 Apr 15;196(1):363-7 PMID: 7306076
  295. Inhibition of lipid peroxidation by the iron-binding protein lactoferrin.
    Biochem J. 1981 Oct 1;199(1):259-61 PMID: 7337708
  296. Identification of ubiquinone-50 in human neutrophils and its role in microbicidal events.
    J Biol Chem. 1982 Jun 25;257(12):6662-8 PMID: 6282820
  297. The enzymic reduction and kinetics of oxidation of cytochrome b-245 of neutrophils.
    Biochem J. 1982 May 15;204(2):479-85 PMID: 7115343
  298. Hydrogen ion currents and intracellular pH in depolarized voltage-clamped snail neurones.
    Nature. 1982 Oct 28;299(5886):826-8 PMID: 7133121
  299. Superoxide-forming NADPH oxidase preparation of pig polymorphonuclear leucocyte.
    Biochem J. 1982 Sep 1;205(3):593-601 PMID: 6293459
  300. Absence of cytochrome b-245 in chronic granulomatous disease. A multicenter European evaluation of its incidence and relevance.
    N Engl J Med. 1983 Feb 3;308(5):245-51 PMID: 6848934
  301. Internal pH of human neutrophil lysosomes.
    FEBS Lett. 1982 Nov 22;149(1):113-6 PMID: 6185362
  302. Development of cytochrome b and an active oxidase system in association with maturation of a human promyelocytic (HL-60) cell line.
    J Cell Biol. 1982 Dec;95(3):720-6 PMID: 6296156
  303. Hydrogen peroxide mediated killing of bacteria.
    Mol Cell Biochem. 1982 Dec 10;49(3):143-9 PMID: 6298593
  304. The association of FAD with the cytochrome b-245 of human neutrophils.
    Biochem J. 1982 Dec 15;208(3):759-63 PMID: 7165731
  305. Structural changes are induced in human neutrophil cytochrome b by NADPH oxidase activators, LDS, SDS, and arachidonate: intermolecular resonance energy transfer between trisulfopyrenyl-wheat germ agglutinin and cytochrome b(558).
    Biochim Biophys Acta. 2002 Dec 23;1567(1-2):221-31 PMID: 12488056
  306. Novel competitive inhibitor of NAD(P)H oxidase assembly attenuates vascular O(2)(-) and systolic blood pressure in mice.
    Circ Res. 2001 Aug 31;89(5):408-14 PMID: 11532901
  307. Phosphorylation of the leucocyte NADPH oxidase subunit p47(phox) by casein kinase 2: conformation-dependent phosphorylation and modulation of oxidase activity.
    Biochem J. 2001 Sep 15;358(Pt 3):783-90 PMID: 11535139
  308. The gp91phox component of NADPH oxidase is not the voltage-gated proton channel in phagocytes, but it helps.
    J Biol Chem. 2001 Sep 28;276(39):36063-6 PMID: 11477065
  309. Goiter and hypothyroidism in two siblings due to impaired Ca(+2)/NAD(P)H-dependent H(2)O(2)-generating activity.
    J Clin Endocrinol Metab. 2001 Oct;86(10):4843-8 PMID: 11600551
  310. Phosphatidylinositol 3-phosphate is generated in phagosomal membranes.
    Curr Biol. 2001 Oct 16;11(20):1631-5 PMID: 11676926
  311. The crystal structure of the PX domain from p40(phox) bound to phosphatidylinositol 3-phosphate.
    Mol Cell. 2001 Oct;8(4):829-39 PMID: 11684018
  312. A fusion protein between rac and p67phox (1-210) reconstitutes NADPH oxidase with higher activity and stability than the individual components.
    Biochemistry. 2001 Nov 20;40(46):14089-97 PMID: 11705402
  313. The NADPH oxidase components p47(phox) and p40(phox) bind to moesin through their PX domain.
    Biochem Biophys Res Commun. 2001 Nov 30;289(2):382-8 PMID: 11716484
  314. Nox/Duox family of nicotinamide adenine dinucleotide (phosphate) oxidases.
    Curr Opin Hematol. 2002 Jan;9(1):11-7 PMID: 11753072
  315. Reactive oxygen generated by Nox1 triggers the angiogenic switch.
    Proc Natl Acad Sci U S A. 2002 Jan 22;99(2):715-20 PMID: 11805326
  316. The p40phox and p47phox PX domains of NADPH oxidase target cell membranes via direct and indirect recruitment by phosphoinositides.
    J Biol Chem. 2002 Feb 8;277(6):4512-8 PMID: 11729195
  317. Determinants of the phagosomal pH in neutrophils.
    J Biol Chem. 2002 Feb 22;277(8):6059-66 PMID: 11744729
  318. The role of ATP-sensitive potassium channels in neutrophil migration and plasma exudation.
    J Pharmacol Exp Ther. 2002 Mar;300(3):946-51 PMID: 11861802
  319. Mapping of functional domains in the p22(phox) subunit of flavocytochrome b(559) participating in the assembly of the NADPH oxidase complex by "peptide walking".
    J Biol Chem. 2002 Mar 8;277(10):8421-32 PMID: 11733522
  320. Chlorination of bacterial and neutrophil proteins during phagocytosis and killing of Staphylococcus aureus.
    J Biol Chem. 2002 Mar 22;277(12):9757-62 PMID: 11733505
  321. Killing activity of neutrophils is mediated through activation of proteases by K+ flux.
    Nature. 2002 Mar 21;416(6878):291-7 PMID: 11907569
  322. Assembly of the neutrophil respiratory burst oxidase: a direct interaction between p67PHOX and cytochrome b558 II.
    Proc Natl Acad Sci U S A. 2002 Apr 2;99(7):4262-5 PMID: 11917128
Article Info
Journal
Biochimica et biophysica acta
Abbr.
Biochim Biophys Acta
ISSN
0006-3002
DOI
10.1016/j.bbabio.2004.03.008
Published
2004-06-28
Pages
1-22
Language
English
Region
Netherlands
NLM ID
0217513
PMCID
PMC2636547
Subset
IM
Grants
Wellcome Trust · 074816 · United Kingdom
External Links
PubMed source DOI: 10.1016/j.bbabio.2004.03.008 PMC Full Text
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2