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PMID: 1850240 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Identification of a superoxide-generating NADPH oxidase system in human fibroblasts.

The Biochemical journal ·Vol. 275 ( Pt 1) ·1991-04-01 ·Pages 241-5

Meier B, Cross AR, Hancock JT, Kaup FJ, Jones OT

Abstract

Human fibroblasts have the capacity to release superoxide radicals upon stimulation of an electron transport system similar to the NADPH oxidase of leukocytes. Two components of the NADPH oxidase system, (1) a flavoprotein of 45 kDa which binds diphenylene iodonium (a compound described as a specific inhibitor of the leukocyte NADPH oxidase), and (2) a low-potential cytochrome b, are present in fibroblast membranes. Fibroblasts exhibit these compounds at lower concentrations than do polymorphonuclear leukocytes or B-lymphocytes. The superoxide-generating system is rather uniformly associated with the outer cell membrane, as shown by light and electron microscopy. Superoxide release upon stimulation with various agents was prevented by the addition of micromolar concentrations of diphenylene iodonium, making an NADPH oxidase a likely source.

MeSH Terms
Calcimycin/pharmacology Cell Membrane/enzymology Cytochrome b Group/metabolism Electron Transport Fibroblasts/enzymology,ultrastructure Free Radicals Humans Hydrogen Peroxide/metabolism Microscopy, Electron, Scanning Molecular Weight NADH, NADPH Oxidoreductases/antagonists & inhibitors,metabolism NADPH Oxidases Onium Compounds/metabolism,pharmacology Superoxides/metabolism
Chemicals
Cytochrome b Group Free Radicals Onium Compounds Superoxides Calcimycin diphenyleneiodonium Hydrogen Peroxide NADH, NADPH Oxidoreductases NADPH Oxidases
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Meier B
Chemisches Institut, Tierärztliche Hochschule, Hannover, Federal Republic of Germany.
Cross A R
Hancock J T
Kaup F J
Jones O T
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj2750241
Published
1991-04-01
Pages
241-5
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1150038
Subset
IM
External Links
PubMed source DOI: 10.1042/bj2750241 PMC Full Text View journal details
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