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PMID: 7506066 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Potential, pH, and arachidonate gate hydrogen ion currents in human neutrophils.

Biophysical journal ·Vol. 65 ·No. 4 ·1993-10-00 ·Pages 1590-8

DeCoursey TE, Cherny VV

Abstract

Indirect evidence indicates that a proton-selective conductance is activated during the respiratory burst in neutrophils. A voltage- and time-dependent H(+)-selective conductance, gH, in human neutrophils is demonstrated here directly by the whole-cell patch-clamp technique. The gH is extremely low at large negative potentials, increases slowly upon membrane depolarization, and does not inactivate. It is enhanced at high external pH or low internal pH and is inhibited by Cd2+ and Zn2+. Arachidonic acid, which plays a pivotal role in inflammatory reactions, amplifies the gH. The properties of the gH described here are compatible with its activation during the respiratory burst in stimulated neutrophils, in which it may facilitate sustained superoxide anion release by dissipating metabolically generated acid.

MeSH Terms
Arachidonic Acid/pharmacology Biophysical Phenomena Biophysics Cadmium/pharmacology Electric Conductivity Humans Hydrogen-Ion Concentration In Vitro Techniques Ion Channel Gating/drug effects Ion Channels/drug effects,metabolism Membrane Potentials Neutrophils/drug effects,metabolism Protons Respiratory Burst Zinc/pharmacology
Chemicals
Ion Channels Protons Cadmium Arachidonic Acid Zinc
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
DeCoursey T E
Department of Physiology, Rush Presbyterian St. Luke's Medical Center, Chicago, Illinois 60612.
Cherny V V
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Article Info
Journal
Biophysical journal
Abbr.
Biophys J
ISSN
0006-3495
DOI
10.1016/S0006-3495(93)81198-6
Published
1993-10-00
Pages
1590-8
Language
English
Region
United States
NLM ID
0370626
PMCID
PMC1225885
Subset
IM
Grants
NHLBI NIH HHS · R01 HL061437 · United States
External Links
PubMed source DOI: 10.1016/S0006-3495(93)81198-6 PMC Full Text View journal details
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