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PMID: 2775188 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Localization of the 47 kDa phosphoprotein involved in the respiratory-burst NADPH oxidase of phagocytic cells.

The Biochemical journal ·Vol. 260 ·No. 1 ·1989-05-15 ·Pages 243-8

Heyworth PG, Shrimpton CF, Segal AW

Abstract

A 47 kDa phosphoprotein is involved in the respiratory-burst oxidase of phagocytic cells. After stimulation of neutrophils with phorbol myristate acetate, this phosphoprotein was identified in both the cytosol and membranes. Peptide mapping of the two forms resulted in identical patterns of phosphopeptides. Dose-response curves for accumulation of phosphoprotein in the two sites were very similar, whereas the detection of the phosphoprotein in the cytosol preceded that in the membranes. The membrane-associated 47 kDa phosphoprotein was absent from the neutrophils of patients with X-chromosome-linked chronic granulomatous disease, which lack cytochrome b-245, and intermediate levels were detected in the membranes of their heterozygote carrier mothers. Activation of the neutrophil oxidase system appears to be dependent upon phosphorylation of the cytosolic 47 kDa protein and its association with cytochrome b-245 in the membranes. It is probably the cytosolic factor required for reconstitution of the active oxidase in cell-free systems.

MeSH Terms
Cell Membrane/analysis Cytosol/analysis Granulomatous Disease, Chronic/metabolism Humans Molecular Weight NADH, NADPH Oxidoreductases/metabolism NADPH Oxidases Neutrophils/analysis,enzymology,ultrastructure Phosphoproteins/analysis Phosphorylation
Chemicals
Phosphoproteins NADH, NADPH Oxidoreductases NADPH Oxidases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Heyworth P G
Department of Medicine, University College London, Rayne Institute, Faculty of Clinical Sciences, U.K.
Shrimpton C F
Segal A W
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27 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj2600243
Published
1989-05-15
Pages
243-8
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1138652
Subset
IM
Grants
Wellcome Trust · United Kingdom
External Links
PubMed source DOI: 10.1042/bj2600243 PMC Full Text View journal details
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