A protein binding the methylated 5'-terminal sequence, m7GpppN, of eukaryotic messenger RNA.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 73 ·No. 5 ·1976-05-00 ·Pages 1559-63

Filipowicz W, Furuichi Y, Sierra JM, Muthukrishnan S, Shatkin AJ, Ochoa S

Abstract

Ribosomal salt washes of Artemia salina embryos contain a protein(s) that binds [3H]m7GpppGpC and [3H]m7GpppGmpC, as measured by retention on nitrocellulose membrane filters. These oligonucleotides correspond in structure to the methylated 5'-terminal sequences (caps) present in many eukaryotic mRNAs. The cap binding protein does not bind the unmethylated counterparts of caps, e.g., [32P]GpppGpCp, or a derivative of m7GpppGmpC containing ring-opened m7G. None of the purified initiation factors IF-MP, IF-M2A, IF-M2B, IF-M3, or IF-MI binds the m7G-containing oligonucleotides.

MeSH Terms

Base Sequence Binding, Competitive Decapoda Guanine Nucleotides/metabolism,pharmacology Magnesium/pharmacology Methylation Peptide Chain Initiation, Translational/drug effects Peptide Initiation Factors Protein Binding/drug effects Protein Biosynthesis/drug effects Proteins/metabolism RNA, Messenger/metabolism Structure-Activity Relationship

Chemicals

Guanine Nucleotides Peptide Initiation Factors Proteins RNA, Messenger Magnesium

Authors & Affiliations

6 authors, click to expand affiliations / ORCID

Filipowicz W

Furuichi Y

Sierra J M

Muthukrishnan S

Shatkin A J

Ochoa S

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26 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.73.5.1559

Published

1976-05-00

Pages

1559-63

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC430337

Subset

External Links

PubMed source DOI: 10.1073/pnas.73.5.1559 PMC Full Text View journal details

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A protein binding the methylated 5'-terminal sequence, m7GpppN, of eukaryotic messenger RNA.

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