Polypeptide chain initiation in eukaryotes: initiation factor MP in Artemia salina embryos.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 72 ·No. 10 ·1975-10-00 ·Pages 3947-51

Filipowicz W, Sierra JM, Ochoa S

Abstract

The activity of IF-MP, a polypeptide chain initiation factor that forms a ternary complex with eukaryotic initiator Met-tRNA and GTP and promotes binding of the initiator to 40S ribosomes, is very low in undeveloped Artemia salina embryos but increases over 20-fold following resumption of development upon hydration of the cysts. The factor is present in both the ribosomal salt wash and high-speed-supernatant. Its specific activity is 50 times higher in the wash but its total activity is only about twice as high in the wash as in the supernatant. As is true of IF-MP from other eukaryotic sources, the A. salina factor is specific for eukaryotic Met-tRNAi and sensitive to SH-reagents, and its activity is GTP dependent.

MeSH Terms

Animals Binding Sites Cytosol/drug effects,metabolism Decapoda Dithiothreitol Embryo, Nonmammalian Escherichia coli/metabolism Ethylmaleimide Guanosine Triphosphate/pharmacology Liver/metabolism Nucleic Acid Conformation Peptide Chain Initiation, Translational Peptide Initiation Factors/analysis RNA, Transfer/metabolism Rabbits Reticulocytes/metabolism Ribosomes/drug effects,metabolism Species Specificity

Chemicals

Peptide Initiation Factors Guanosine Triphosphate RNA, Transfer Ethylmaleimide Dithiothreitol

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Filipowicz W

Sierra J M

Ochoa S

References (22)

22 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.72.10.3947

Published

1975-10-00

Pages

3947-51

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC433114

Subset

External Links

PubMed source DOI: 10.1073/pnas.72.10.3947 PMC Full Text View journal details

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Polypeptide chain initiation in eukaryotes: initiation factor MP in Artemia salina embryos.

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