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PMID: 1061126 Published · ppublish English Journal Article

Polypeptide chain initiation in eukaryotes: initiation factor requirements for translation of natural messengers.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 73 ·No. 1 ·1976-01-00 ·Pages 44-8

Filipowicz W, Sierra JM, Nombela C, Ochoa S, Merrick WC, Anderson WF

Abstract

A protein-synthesizing system consisting of ribosomes and supernatant of undeveloped Artemia saline embryos is used for assay of mRNA translation and initiation factors. This system contains the components needed for chain elongation but has low levels of mRNA and initiation factors. Exogenous mRNA is readily translated upon addition of high-speed supernatant or ribosomal salt wash of developing embryos as a source of initiation factors (IF). This requirement can be largely satisfied by a mixture of the reticulocyte factors IF-MP, IF-M3, IF-M2A, and IF-M2B. A. salina IF-M1, which is present in undeveloped embryo supernatant, can be inactivated by A. salina IF-M1 antibody without affecting translation.

MeSH Terms
Animals Antigen-Antibody Reactions Cell-Free System Decapoda/embryology Globins Peptide Chain Initiation, Translational Peptide Initiation Factors/analysis Protein Biosynthesis RNA, Messenger/metabolism Reticulocytes Ribosomes/metabolism
Chemicals
Peptide Initiation Factors RNA, Messenger Globins
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Filipowicz W
Sierra J M
Nombela C
Ochoa S
Merrick W C
Anderson W F
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21 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.73.1.44
Published
1976-01-00
Pages
44-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC335835
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.73.1.44 PMC Full Text View journal details
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