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PMID: 1061116 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Inhibition of initiation of protein synthesis by 7-methylguanosine-5'-monophosphate.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 73 ·No. 1 ·1976-01-00 ·Pages 19-23

Hickey ED, Weber LA, Baglioni C

Abstract

Translation of rabbit globin mRNA in a wheat germ protein-synthesizing system is inhibited by the nucleotide 7-methylguanosine-5'-monophosphate (m7G5'p) but not by other guanosine nucleotides without the 7-methyl group or with the phosphate in a different position. Translation of RNA of tobacco mosaic virus and poly(A) + HeLa RNA is also inhibited by m7G5'p. We show that m7G5'p prevents the association of mRNA with ribosomal subunits to form an initiation complex. We propose that m7G5'p interacts with a site on initiation factor(s) or ribosomes which is involved in mRNA recognition, presumably by binding to the 5'-terminal sequence m7G5'ppp. m7G5'p does not inhibit translation of poly(U) and RNA of satellite tobacco necrosis virus, which do not have the 5'-terminal sequence m7G5'ppp. In the case of RNA of satellite tobacco necrosis virus, some stimulation of its translation is consistently observed in the presence of m7G5'p; possible interpretations of this finding are discussed.

MeSH Terms

Cell-Free System Depression, Chemical Globulins/biosynthesis Guanine Nucleotides/pharmacology HeLa Cells Peptide Chain Initiation, Translational/drug effects Plant Viruses RNA Cap Analogs RNA, Messenger/metabolism RNA, Viral/metabolism Reoviridae Ribosomes/metabolism Satellite Viruses Tobacco Mosaic Virus

Chemicals

Globulins Guanine Nucleotides RNA Cap Analogs RNA, Messenger RNA, Viral 7-methylguanosine-5'-monophosphate

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Hickey E D

Weber L A

Baglioni C

References (22)

22 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.73.1.19

Published

1976-01-00

Pages

19-23

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC335830

Subset

External Links

PubMed source DOI: 10.1073/pnas.73.1.19 PMC Full Text View journal details

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Inhibition of initiation of protein synthesis by 7-methylguanosine-5'-monophosphate.

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