5'-Terminal 7-methylguanosine in eukaryotic mRNA is required for translation.

Nature ·Vol. 255 ·No. 5503 ·1975-05-01 ·Pages 33-7

Muthukrishnan S, Both GW, Furuichi Y, Shatkin AJ

Abstract

Unmethylated reovirus and VSV mRNAs are specifically methylated to form 5'-terminal structures of the type, m-7-G(5')ppp(5')N by protein synthesising extracts prepared from wheat germ and mouse L cells. Reticulocyte mRNA also contains 5'-terminal m-7-G. MRNAs having 5'-terminal m-7-G stimulate protein synthesis in vitro. Removal of m-7-G by beta-elimination abolishes translation of the mRNAs.

MeSH Terms

Animals Cell-Free System Electrophoresis, Paper Guanosine/metabolism Humans L Cells/metabolism Methylation Plant Proteins/biosynthesis Protein Biosynthesis RNA, Messenger/metabolism RNA, Viral/metabolism Rabbits Reoviridae/metabolism Reticulocytes/metabolism Triticum/metabolism Tritium Vesicular stomatitis Indiana virus/metabolism Viral Proteins/biosynthesis Virus Replication

Chemicals

Plant Proteins RNA, Messenger RNA, Viral Viral Proteins Tritium Guanosine

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Muthukrishnan S

Both G W

Furuichi Y

Shatkin A J

Article Info

Journal

Nature

Abbr.

Nature

ISSN

0028-0836

DOI

10.1038/255033a0

Published

1975-05-01

Pages

33-7

Language

English

Region

England

NLM ID

0410462

Subset

External Links

PubMed source DOI: 10.1038/255033a0 View journal details

Analysis Services

5'-Terminal 7-methylguanosine in eukaryotic mRNA is required for translation.

Contact

WeChat Official Account