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PMID: 4528644 Published · ppublish English Journal Article

Methylated messenger RNA synthesis in vitro by purified reovirus.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 71 ·No. 8 ·1974-08-00 ·Pages 3204-7

Shatkin AJ

Abstract

Purified human reovirus contains RNA methylase activity in addition to an RNA polymerase. Virions incubated under appropriate conditions in the presence of S-adenosyl-L-methionine synthesize mRNA that is specifically methylated in the 5'-terminal guanosine. Alkaline digestion of the methylated RNA released a 5'-terminal dinucleotide, ppG'pCp, indicating that the guanosine contains 2'-O-methylribose. The possible roles of methylation in viral and cellular mRNA function is discussed.

MeSH Terms
Chromatography, DEAE-Cellulose Culture Media DNA-Directed RNA Polymerases/metabolism Kinetics L Cells/metabolism Methylation Oligonucleotides/isolation & purification Phosphorus Radioisotopes RNA, Messenger/biosynthesis,metabolism Reoviridae/enzymology,metabolism S-Adenosylmethionine/metabolism Stimulation, Chemical Tritium Valine/metabolism tRNA Methyltransferases/metabolism
Chemicals
Culture Media Oligonucleotides Phosphorus Radioisotopes RNA, Messenger Tritium S-Adenosylmethionine tRNA Methyltransferases DNA-Directed RNA Polymerases Valine
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Shatkin A J
References (16)
16 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.71.8.3204
Published
1974-08-00
Pages
3204-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC388651
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.71.8.3204 PMC Full Text View journal details
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