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PMID: 5723704 Published · ppublish English Journal Article

Separation of ten reovirus genome segments by polyacrylamide gel electrophoresis.

Journal of virology ·Vol. 2 ·No. 10 ·1968-10-00 ·Pages 986-91

Shatkin AJ, Sipe JD, Loh P

Abstract

Double-stranded ribonucleic acid (RNA) extracted from purified reoviruses of all three serotypes and from type 3 virus-infected cells was analyzed by polyacrylamide gel electrophoresis. It was calculated that each RNA includes 10 segments: 3 large, 3 intermediate, and 4 small fragments corresponding to molecular weights of about 2.5, 1.4, and 0.8 x 10(6) daltons, respectively, or a total of 15 x 10(6) daltons.

MeSH Terms
Acrylates Electrophoresis Gels Genetics, Microbial RNA, Viral/analysis Reoviridae/analysis
Chemicals
Acrylates Gels RNA, Viral
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Shatkin A J
Sipe J D
Loh P
References (22)
22 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.2.10.986-991.1968
Published
1968-10-00
Pages
986-91
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC375428
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.2.10.986-991.1968 PMC Full Text View journal details
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