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PMID: 10793759 Published · ppublish English Journal Article

"Methylation-coupled" transcription by virus-associated transcriptase of cytoplasmic polyhedrosis virus containing double-stranded RNA.

Nucleic acids research ·Vol. 1 ·No. 6 ·1974-06-00 ·Pages 809-22

Furuichi Y

Abstract

S-adenosyl-L-methionine (SAM) activated the virus-associated RNA polymerase of cytoplasmic polyhedrosis virus in vitro. Synthesis of single-stranded viral RNA (mRNA) proceeded depending on the presence of SAM.A methyl residue of SAM was incorporated into an RNA molecule. A ribose moiety of adenylic acid in the 5'-terminal region of the nascent RNA was methylated in the very early stage of the transcription. The dependence of the viral transcription on the presence of SAM and the methylation of terminal nucleotide suggests that the transcription of CPV is a "methylation-coupled" reaction.

MeSH Terms
DNA-Directed RNA Polymerases/metabolism Methylation RNA, Double-Stranded/genetics,metabolism RNA, Messenger/genetics,metabolism RNA, Viral/genetics,metabolism Reoviridae/genetics,metabolism S-Adenosylmethionine/metabolism Transcription, Genetic
Chemicals
RNA, Double-Stranded RNA, Messenger RNA, Viral S-Adenosylmethionine DNA-Directed RNA Polymerases
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Furuichi Y
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24 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/1.6.809
Published
1974-06-00
Pages
809-22
Language
English
Region
England
NLM ID
0411011
PMCID
PMC343382
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/1.6.809 PMC Full Text View journal details
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