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PMID: 9858548 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The msh2 gene of Schizosaccharomyces pombe is involved in mismatch repair, mating-type switching, and meiotic chromosome organization.

Molecular and cellular biology ·Vol. 19 ·No. 1 ·1999-01-00 ·Pages 241-50

Rudolph C, Kunz C, Parisi S, Lehmann E, Hartsuiker E, Fartmann B, Kramer W, Kohli J, Fleck O

Abstract

We have identified in the fission yeast Schizosaccharomyces pombe a MutS homolog that shows highest homology to the Msh2 subgroup. msh2 disruption gives rise to increased mitotic mutation rates and increased levels of postmeiotic segregation of genetic markers. In bandshift assays performed with msh2Delta cell extracts, a general mismatch-binding activity is absent. By complementation assays, we showed that S. pombe msh2 is allelic with the previously identified swi8 and mut3 genes, which are involved in mating-type switching. The swi8-137 mutant has a mutation in the msh2 gene which causes a truncated Msh2 peptide lacking a putative DNA-binding domain. Cytological analysis revealed that during meiotic prophase of msh2-defective cells, chromosomal structures were frequently formed; such structures are rarely found in the wild type. Our data show that besides having a function in mismatch repair, S. pombe msh2 is required for correct termination of copy synthesis during mating-type switching as well as for proper organization of chromosomes during meiosis.

MeSH Terms
Alleles Base Pair Mismatch Base Sequence Chromosomes, Fungal Cloning, Molecular DNA Repair DNA, Fungal DNA-Binding Proteins/genetics Fungal Proteins Genes, Fungal Genes, Mating Type, Fungal Meiosis Mitosis Molecular Sequence Data MutS Homolog 2 Protein Nucleic Acid Conformation Prophase Schizosaccharomyces/genetics Schizosaccharomyces pombe Proteins Spores, Fungal
Chemicals
DNA, Fungal DNA-Binding Proteins Fungal Proteins Schizosaccharomyces pombe Proteins MutS Homolog 2 Protein msh2 protein, S pombe
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Rudolph C
Institute of General Microbiology, University of Bern, CH-3012 Bern, Switzerland.
Kunz C
Parisi S
Lehmann E
Hartsuiker E
Fartmann B
Kramer W
Kohli J
Fleck O
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.19.1.241
Published
1999-01-00
Pages
241-50
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC83882
Subset
IM
Databases
GENBANK
AJ006948
External Links
PubMed source DOI: 10.1128/MCB.19.1.241 PMC Full Text View journal details
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