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PMID: 8462843 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Meiotic mismatch repair quantified on the basis of segregation patterns in Schizosaccharomyces pombe.

Genetics ·Vol. 133 ·No. 4 ·1993-04-00 ·Pages 815-24

Schär P, Munz P, Kohli J

Abstract

Hybrid DNA with mismatched base pairs is a central intermediate of meiotic recombination. Mismatch repair leads either to restoration or conversion, while failure of repair results in postmeiotic segregation (PMS). The behavior of three G to C transversions in one-factor crosses with the wild-type alleles is studied in Schizosaccharomyces pombe. They lead to C/C and G/G mismatches and are compared with closely linked mutations yielding other mismatches. A method is presented for the detection of PMS in random spores. The procedure yields accurate PMS frequencies as shown by comparison with tetrad data. A scheme is presented for the calculation of the frequency of hybrid DNA formation and the efficiency of mismatch repair. The efficiency of C/C repair in S. pombe is calculated to be about 70%. Other mismatches are repaired with close to 100% efficiency. These results are compared with data published on mutations in Saccharomyces cerevisiae and Ascobolus immersus. This study forms the basis for the detailed analysis of the marker effects caused by G to C transversions in two-factor crosses.

Related Genes

ade6 sup3

MeSH Terms

Ascomycota/genetics Chromatids Chromosomes, Fungal DNA Repair/genetics DNA, Fungal/genetics Meiosis/genetics Mutation Nucleic Acid Heteroduplexes Recombination, Genetic Saccharomyces cerevisiae/genetics Schizosaccharomyces/cytology,genetics Spores, Fungal

Chemicals

DNA, Fungal Nucleic Acid Heteroduplexes

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Schär P

Institute of General Microbiology, University of Bern, Switzerland.

Munz P

Kohli J

References (23)

23 references, click to expand

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Article Info

Journal

Genetics

Abbr.

Genetics

ISSN

0016-6731

DOI

10.1093/genetics/133.4.815

Published

1993-04-00

Pages

815-24

Language

English

Region

United States

NLM ID

0374636

PMCID

PMC1205402

Subset

External Links

PubMed source DOI: 10.1093/genetics/133.4.815 PMC Full Text View journal details

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Meiotic mismatch repair quantified on the basis of segregation patterns in Schizosaccharomyces pombe.

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