Home LiteratureArticle Details
PMID: 9258673 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Mismatch repair in Schizosaccharomyces pombe requires the mutL homologous gene pms1: molecular cloning and functional analysis.

Genetics ·Vol. 146 ·No. 4 ·1997-08-00 ·Pages 1275-86

Schär P, Baur M, Schneider C, Kohli J

Abstract

Homologues of the bacterial mutS and mutL genes involved in DNA mismatch repair have been found in organisms from bacteria to humans. Here, we describe the structure and function of a newly identified Schizosaccharomyces pombe that encodes a predicted amino acid sequence of 794 residues with a high degree of homology to MutL related proteins. On the basis of its closer relationship to the eukaryotic "PMS" genes than to the "MLH" genes, we have designated the S. pombe homologue pms1. Disruption of the pms1 gene causes a significant increase of spontaneous mutagenesis as documented by reversion rate measurements. Tetrad analyses of crosses homozygous for the pms1 mutation reveal a reduction of spore viability from > 92% to 80% associated with a low proportion (approximately 50%) of meioses producing four viable spores and a significant, allele-dependent increase of the level of post-meiotic segregation of genetic marker allele pairs. The mutant phenotypes are consistent with a general function of pms1 in correction of mismatched base pairs arising as a consequence of DNA polymerase errors during DNA synthesis, or of hybrid DNA formation between homologous but not perfectly complementary DNA strands during meiotic recombination.

MeSH Terms
Adenosine Triphosphatases Amino Acid Sequence Bacterial Proteins/genetics Base Sequence Carrier Proteins Chromosome Mapping Cloning, Molecular DNA Primers/genetics DNA Repair/genetics DNA, Fungal/genetics Escherichia coli Proteins Fungal Proteins/genetics Genes, Fungal Meiosis/genetics Mitosis/genetics Molecular Sequence Data MutL Proteins Mutation Phenotype Polymerase Chain Reaction Schizosaccharomyces/cytology,genetics,metabolism Sequence Homology, Amino Acid Species Specificity Spores, Fungal/genetics
Chemicals
Bacterial Proteins Carrier Proteins DNA Primers DNA, Fungal Escherichia coli Proteins Fungal Proteins MutL protein, E coli Adenosine Triphosphatases MutL Proteins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Schär P
Institute of Medical Radiobiology, University of Zürich, Switzerland. schaer@imr.unizh.ch
Baur M
Schneider C
Kohli J
References (53)
53 references, click to expand
  1. The yeast gene MSH3 defines a new class of eukaryotic MutS homologues.
    Mol Gen Genet. 1993 May;239(1-2):97-108 PMID: 8510668
  2. Meiotic gene conversion mutants in Saccharomyces cerevisiae. I. Isolation and characterization of pms1-1 and pms1-2.
    Genetics. 1985 Aug;110(4):609-46 PMID: 3896926
  3. Mismatch recognition in chromosomal interactions and speciation.
    Chromosoma. 1993 Jun;102(6):369-73 PMID: 8365347
  4. Nonconserved segment of the MutL protein from Escherichia coli K-12 and Salmonella typhimurium.
    Nucleic Acids Res. 1992 May 11;20(9):2379 PMID: 1594459
  5. Polarity of meiotic gene conversion in fungi: contrasting views.
    Experientia. 1994 Mar 15;50(3):242-52 PMID: 8143798
  6. The nucleotide mapping of DNA double-strand breaks at the CYS3 initiation site of meiotic recombination in Saccharomyces cerevisiae.
    EMBO J. 1995 Sep 15;14(18):4589-98 PMID: 7556102
  7. Alignment of Sfi I sites with the Not I restriction map of Schizosaccharomyces pombe genome.
    Nucleic Acids Res. 1991 Nov 25;19(22):6289-94 PMID: 1956788
  8. Isolation and characterization of two Saccharomyces cerevisiae genes encoding homologs of the bacterial HexA and MutS mismatch repair proteins.
    Genetics. 1992 Dec;132(4):963-73 PMID: 1459447
  9. hMSH2 forms specific mispair-binding complexes with hMSH3 and hMSH6.
    Proc Natl Acad Sci U S A. 1996 Nov 26;93(24):13629-34 PMID: 8942985
  10. DNA sequence analysis of the ade6 gene of Schizosaccharomyces pombe. Wild-type and mutant alleles including the recombination host spot allele ade6-M26.
    J Mol Biol. 1988 Dec 20;204(4):917-25 PMID: 3221399
  11. Involvement of mouse Mlh1 in DNA mismatch repair and meiotic crossing over.
    Nat Genet. 1996 Jul;13(3):336-42 PMID: 8673133
  12. Restoration of mismatch repair to nuclear extracts of H6 colorectal tumor cells by a heterodimer of human MutL homologs.
    Proc Natl Acad Sci U S A. 1995 Mar 14;92(6):1950-4 PMID: 7892206
  13. Human strand-specific mismatch repair occurs by a bidirectional mechanism similar to that of the bacterial reaction.
    J Biol Chem. 1993 Jun 5;268(16):11838-44 PMID: 8505312
  14. Identification of two mismatch-binding activities in protein extracts of Schizosaccharomyces pombe.
    Nucleic Acids Res. 1994 Dec 11;22(24):5289-95 PMID: 7816618
  15. High resolution cosmid and P1 maps spanning the 14 Mb genome of the fission yeast S. pombe.
    Cell. 1993 Apr 9;73(1):109-20 PMID: 8462094
  16. Destabilization of tracts of simple repetitive DNA in yeast by mutations affecting DNA mismatch repair.
    Nature. 1993 Sep 16;365(6443):274-6 PMID: 8371783
  17. Mutations of a mutS homolog in hereditary nonpolyposis colorectal cancer.
    Cell. 1993 Dec 17;75(6):1215-25 PMID: 8261515
  18. Meiotic mismatch repair quantified on the basis of segregation patterns in Schizosaccharomyces pombe.
    Genetics. 1993 Apr;133(4):815-24 PMID: 8462843
  19. The swi4+ gene of Schizosaccharomyces pombe encodes a homologue of mismatch repair enzymes.
    Nucleic Acids Res. 1992 May 11;20(9):2271-8 PMID: 1317550
  20. Site Specific Induction of Gene Conversion in SCHIZOSACCHAROMYCES POMBE.
    Genetics. 1971 Nov;69(3):317-37 PMID: 17248549
  21. Transformation of Schizosaccharomyces pombe by non-homologous, unstable integration of plasmids in the genome.
    Curr Genet. 1986;10(7):503-8 PMID: 3442827
  22. Genetic and physical analysis of the M26 recombination hotspot of Schizosaccharomyces pombe.
    Genetics. 1988 Jul;119(3):491-7 PMID: 3402730
  23. Versatile shuttle vectors and genomic libraries for use with Schizosaccharomyces pombe.
    Gene. 1992 May 1;114(1):59-66 PMID: 1587485
  24. The Saccharomyces cerevisiae Msh2 protein specifically binds to duplex oligonucleotides containing mismatched DNA base pairs and insertions.
    Genes Dev. 1995 Jan 15;9(2):234-47 PMID: 7851796
  25. Genetic engineering of Schizosaccharomyces pombe: a system for gene disruption and replacement using the ura4 gene as a selectable marker.
    Mol Gen Genet. 1988 Dec;215(1):81-6 PMID: 3241624
  26. Interaction between mismatch repair and genetic recombination in Saccharomyces cerevisiae.
    Genetics. 1994 May;137(1):19-39 PMID: 8056309
  27. Mutations of two PMS homologues in hereditary nonpolyposis colon cancer.
    Nature. 1994 Sep 1;371(6492):75-80 PMID: 8072530
  28. Characterization of insertion mutations in the Saccharomyces cerevisiae MSH1 and MSH2 genes: evidence for separate mitochondrial and nuclear functions.
    Genetics. 1992 Dec;132(4):975-85 PMID: 1334021
  29. Hotspots of homologous recombination.
    Experientia. 1994 Mar 15;50(3):234-41 PMID: 8143797
  30. On some properties of five mutator alleles in Schizosaccharomyces pombe.
    Mutat Res. 1975 Jul;29(1):155-7 PMID: 1152857
  31. Preferential strand transfer and hybrid DNA formation at the recombination hotspot ade6-M26 of Schizosaccharomyces pombe.
    EMBO J. 1994 Nov 1;13(21):5212-9 PMID: 7957086
  32. Inactivation of the mouse Msh2 gene results in mismatch repair deficiency, methylation tolerance, hyperrecombination, and predisposition to cancer.
    Cell. 1995 Jul 28;82(2):321-30 PMID: 7628020
  33. Nucleotide sequence of the Streptococcus pneumoniae hexB mismatch repair gene: homology of HexB to MutL of Salmonella typhimurium and to PMS1 of Saccharomyces cerevisiae.
    J Bacteriol. 1989 Oct;171(10):5332-8 PMID: 2676973
  34. Male mice defective in the DNA mismatch repair gene PMS2 exhibit abnormal chromosome synapsis in meiosis.
    Cell. 1995 Jul 28;82(2):309-19 PMID: 7628019
  35. Initiation of methyl-directed mismatch repair.
    J Biol Chem. 1992 Jun 15;267(17):12142-8 PMID: 1601880
  36. Methyl-directed mismatch repair is bidirectional.
    J Biol Chem. 1993 Jun 5;268(16):11823-9 PMID: 8389365
  37. MLH1, PMS1, and MSH2 interactions during the initiation of DNA mismatch repair in yeast.
    Science. 1994 Aug 19;265(5175):1091-3 PMID: 8066446
  38. Mutation of a mutL homolog in hereditary colon cancer.
    Science. 1994 Mar 18;263(5153):1625-9 PMID: 8128251
  39. Mechanisms and biological effects of mismatch repair.
    Annu Rev Genet. 1991;25:229-53 PMID: 1812808
  40. GTBP, a 160-kilodalton protein essential for mismatch-binding activity in human cells.
    Science. 1995 Jun 30;268(5219):1912-4 PMID: 7604265
  41. Cloning and nucleotide sequence of DNA mismatch repair gene PMS1 from Saccharomyces cerevisiae: homology of PMS1 to procaryotic MutL and HexB.
    J Bacteriol. 1989 Oct;171(10):5339-46 PMID: 2676974
  42. Interallelic and intergenic conversion in three serine tRNA genes of Schizosaccharomyces pombe.
    Cold Spring Harb Symp Quant Biol. 1984;49:31-40 PMID: 6597758
  43. Dual requirement in yeast DNA mismatch repair for MLH1 and PMS1, two homologs of the bacterial mutL gene.
    Mol Cell Biol. 1994 Jan;14(1):407-15 PMID: 8264608
  44. The double-strand-break repair model for recombination.
    Cell. 1983 May;33(1):25-35 PMID: 6380756
  45. Complete coverage of the Schizosaccharomyces pombe genome in yeast artificial chromosomes.
    Nat Genet. 1992 Jul;1(4):273-7 PMID: 1302023
  46. Redundancy of Saccharomyces cerevisiae MSH3 and MSH6 in MSH2-dependent mismatch repair.
    Genes Dev. 1996 Feb 15;10(4):407-20 PMID: 8600025
  47. Maximum likelihood estimation of linkage and interference from tetrad data.
    Genetics. 1979 May;92(1):231-45 PMID: 17248917
  48. A role for exonuclease I from S. pombe in mutation avoidance and mismatch correction.
    Science. 1995 Feb 24;267(5201):1166-9 PMID: 7855597
  49. Strand-specific mismatch correction in nuclear extracts of human and Drosophila melanogaster cell lines.
    Proc Natl Acad Sci U S A. 1990 Aug;87(15):5837-41 PMID: 2116007
  50. Mutation in the DNA mismatch repair gene homologue hMLH1 is associated with hereditary non-polyposis colon cancer.
    Nature. 1994 Mar 17;368(6468):258-61 PMID: 8145827
  51. 3' non-coding region sequences in eukaryotic messenger RNA.
    Nature. 1976 Sep 16;263(5574):211-4 PMID: 822353
  52. Isolation of an hMSH2-p160 heterodimer that restores DNA mismatch repair to tumor cells.
    Science. 1995 Jun 30;268(5219):1909-12 PMID: 7604264
  53. Architectural features of pre-mRNA introns in the fission yeast Schizosaccharomyces pombe.
    Yeast. 1992 Mar;8(3):171-82 PMID: 1574925
Article Info
Journal
Genetics
Abbr.
Genetics
ISSN
0016-6731
DOI
10.1093/genetics/146.4.1275
Email
schaer@imr.unizh.ch
Published
1997-08-00
Pages
1275-86
Language
English
Region
United States
NLM ID
0374636
PMCID
PMC1208074
Subset
IM
Databases
GENBANK
M29688, U07187, U07343, U13695, U13696, U28724, X96581
External Links
PubMed source DOI: 10.1093/genetics/146.4.1275 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2