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PMID: 7816618 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Identification of two mismatch-binding activities in protein extracts of Schizosaccharomyces pombe.

Nucleic acids research ·Vol. 22 ·No. 24 ·1994-12-11 ·Pages 5289-95

Fleck O, Schär P, Kohli J

Abstract

We have performed band-shift assays to identify mismatch-binding proteins in cell extracts of Schizosaccharomyces pombe. By testing heteroduplex DNA containing either a T/G or a C/C mismatch, two distinct band shifts were produced in the gels. A low mobility complex was observed with the T/G substrate, while a high mobility complex was present with C/C. Further analysis of the mismatch-binding specificities revealed that the T/G binding activity also binds to T/C, C/T, T/T, T/-, A/-, C/-, G/-, G/G, A/A, A/C, A/G, G/T, G/A, and C/A substrates with varying efficiencies, but not binds to C/C. The C/C binding activity efficiently binds to C/C, T/C, C/T, C/A, A/C, C/-, and weakly also to T/T, while all other mispairs are not recognized. Protein extracts of a mutant strain, defective in the mutS homologue swi4, displayed both mismatch-binding activities. Thus, swi4 does not encode for either one of the mismatch-binding proteins.

Related Genes
swi4
MeSH Terms
Base Sequence DNA, Fungal/metabolism DNA, Single-Stranded/chemical synthesis,metabolism DNA-Binding Proteins/metabolism Fungal Proteins/genetics,metabolism Molecular Sequence Data Mutation Nucleic Acid Heteroduplexes/metabolism Polydeoxyribonucleotides/chemical synthesis,metabolism Saccharomyces cerevisiae Proteins Schizosaccharomyces/metabolism Transcription Factors
Chemicals
DNA, Fungal DNA, Single-Stranded DNA-Binding Proteins Fungal Proteins Nucleic Acid Heteroduplexes Polydeoxyribonucleotides SWI4 protein, S cerevisiae Saccharomyces cerevisiae Proteins Transcription Factors
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Fleck O
Institute of General Microbiology, University of Bern, Switzerland.
Schär P
Kohli J
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/22.24.5289
Published
1994-12-11
Pages
5289-95
Language
English
Region
England
NLM ID
0411011
PMCID
PMC332073
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/22.24.5289 PMC Full Text View journal details
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