Home LiteratureArticle Details
PMID: 1317550 Published · ppublish English Journal Article

The swi4+ gene of Schizosaccharomyces pombe encodes a homologue of mismatch repair enzymes.

Nucleic acids research ·Vol. 20 ·No. 9 ·1992-05-11 ·Pages 2271-8

Fleck O, Michael H, Heim L

Abstract

The swi4+ gene of Schizosaccharomyces pombe is involved in termination of copy-synthesis during mating-type switching. The gene was cloned by functional complementation of a swi4 mutant transformed with a genomic library. Determination of the nucleotide sequence revealed an open reading frame of 2979 nucleotides which is interrupted by a 68 bp long intron. The putative Swi4 protein shows homology to Duc-1 (human), Rep-3 (mouse), HexA (Streptococcus pneumoniae) and MutS (Salmonella typhimurium). The prokaryotic proteins are known as essential components involved in mismatch repair. A strain with a disrupted swi4+ gene was constructed and analysed with respect to the switching process. As in swi4 mutants duplications occur in the mating-type region of the swi4 (null) strain, reducing the efficiency of switching.

Related Genes
swi4<up>+</up>
MeSH Terms
Adenosine Triphosphatases Amino Acid Sequence Animals Bacterial Proteins/chemistry Base Sequence Blotting, Northern Blotting, Southern Cloning, Molecular DNA Repair/genetics DNA-Binding Proteins Escherichia coli Proteins Fungal Proteins/chemistry,genetics Genes, Fungal Genes, Mating Type, Fungal Genes, Switch Humans Molecular Sequence Data MutS DNA Mismatch-Binding Protein Restriction Mapping Saccharomyces cerevisiae Proteins Schizosaccharomyces/enzymology,genetics Sequence Homology, Nucleic Acid Transcription Factors
Chemicals
Bacterial Proteins DNA-Binding Proteins Escherichia coli Proteins Fungal Proteins SWI4 protein, S cerevisiae Saccharomyces cerevisiae Proteins Transcription Factors Adenosine Triphosphatases MutS DNA Mismatch-Binding Protein MutS protein, E coli
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Fleck O
Institut für Genetik, Biozentrum, Technische Universität Braunschweig, Germany.
Michael H
Heim L
References (42)
42 references, click to expand
  1. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  2. Distantly related sequences in the alpha- and beta-subunits of ATP synthase, myosin, kinases and other ATP-requiring enzymes and a common nucleotide binding fold.
    EMBO J. 1982;1(8):945-51 PMID: 6329717
  3. Targeting of T7 RNA polymerase to tobacco nuclei mediated by an SV40 nuclear location signal.
    Plant Mol Biol. 1991 Aug;17(2):229-34 PMID: 1650616
  4. Altering the conserved nucleotide binding motif in the Salmonella typhimurium MutS mismatch repair protein affects both its ATPase and mismatch binding activities.
    EMBO J. 1991 Sep;10(9):2707-15 PMID: 1651234
  5. The sequence of a 6.3 kb segment of yeast chromosome III reveals an open reading frame coding for a putative mismatch binding protein.
    Yeast. 1991 Dec;7(9):981-8 PMID: 1803822
  6. The rate of nuclear cytoplasmic protein transport is determined by the casein kinase II site flanking the nuclear localization sequence of the SV40 T-antigen.
    EMBO J. 1991 Mar;10(3):633-9 PMID: 1848177
  7. The mechanism of fission yeast mating type interconversion: seal/replicate/cleave model of replication across the double-stranded break site at mat1.
    Genetics. 1991 Mar;127(3):489-96 PMID: 2016051
  8. A mutated swi4 gene causes duplications in the mating-type region of Schizosaccharomyces pombe.
    Curr Genet. 1990 Dec;18(6):501-9 PMID: 2076550
  9. Relocalization of the dorsal protein from the cytoplasm to the nucleus correlates with its function.
    Cell. 1989 Dec 22;59(6):1179-88 PMID: 2598266
  10. Some of the swi genes of Schizosaccharomyces pombe also have a function in the repair of radiation damage.
    Curr Genet. 1989 Aug;16(2):89-94 PMID: 2598273
  11. Dual bidirectional promoters at the mouse dhfr locus: cloning and characterization of two mRNA classes of the divergently transcribed Rep-1 gene.
    Mol Cell Biol. 1989 Jul;9(7):3058-72 PMID: 2674679
  12. Isolation and characterization of cDNA clones derived from the divergently transcribed gene in the region upstream from the human dihydrofolate reductase gene.
    J Biol Chem. 1989 Jun 15;264(17):10057-64 PMID: 2722860
  13. Mechanisms of nuclear localization of the progesterone receptor: evidence for interaction between monomers.
    Cell. 1989 Jun 30;57(7):1147-54 PMID: 2736623
  14. Four mating-type genes control sexual differentiation in the fission yeast.
    EMBO J. 1988 May;7(5):1537-47 PMID: 2900761
  15. Identification and characterization of the mutL and mutS gene products of Salmonella typhimurium LT2.
    J Bacteriol. 1985 Sep;163(3):1007-15 PMID: 2993227
  16. Nuclear location signals in polyoma virus large-T.
    Cell. 1986 Jan 17;44(1):77-85 PMID: 3000623
  17. Escherichia coli mutS-encoded protein binds to mismatched DNA base pairs.
    Proc Natl Acad Sci U S A. 1986 Jul;83(14):5057-61 PMID: 3014530
  18. Replicating plasmids in Schizosaccharomyces pombe: improvement of symmetric segregation by a new genetic element.
    Mol Cell Biol. 1986 Jan;6(1):80-9 PMID: 3023839
  19. A new cell division operon in Escherichia coli.
    Mol Gen Genet. 1986 Oct;205(1):134-45 PMID: 3025556
  20. Casein kinase II as a potentially important enzyme concerned with signal transduction.
    Cold Spring Harb Symp Quant Biol. 1988;53 Pt 1:77-84 PMID: 3076090
  21. Dorsal, an embryonic polarity gene in Drosophila, is homologous to the vertebrate proto-oncogene, c-rel.
    Science. 1987 Oct 30;238(4827):692-4 PMID: 3118464
  22. Genetic engineering of Schizosaccharomyces pombe: a system for gene disruption and replacement using the ura4 gene as a selectable marker.
    Mol Gen Genet. 1988 Dec;215(1):81-6 PMID: 3241624
  23. CLUSTAL: a package for performing multiple sequence alignment on a microcomputer.
    Gene. 1988 Dec 15;73(1):237-44 PMID: 3243435
  24. Nucleotide sequence of the hexA gene for DNA mismatch repair in Streptococcus pneumoniae and homology of hexA to mutS of Escherichia coli and Salmonella typhimurium.
    J Bacteriol. 1988 Jan;170(1):190-6 PMID: 3275608
  25. Transformation of intact yeast cells treated with alkali cations.
    J Bacteriol. 1983 Jan;153(1):163-8 PMID: 6336730
  26. Plasmids carrying the yeast OMP decarboxylase structural and regulatory genes: transcription regulation in a foreign environment.
    Cell. 1983 Feb;32(2):371-7 PMID: 6337726
  27. Genes required for initiation and resolution steps of mating-type switching in fission yeast.
    Proc Natl Acad Sci U S A. 1984 Jun;81(11):3481-5 PMID: 6587363
  28. Methodological Information on the Genetics of Schizosaccharomyces Pombe.
    Schweiz Z Pathol Bakteriol. 1955;18(5):1141-6 PMID: 13311482
  29. Mapping the double-strand breaks at the mating-type locus in fission yeast by genomic sequencing.
    EMBO J. 1989 Jan;8(1):269-76 PMID: 2714252
  30. Nucleotide sequence of the Salmonella typhimurium mutS gene required for mismatch repair: homology of MutS and HexA of Streptococcus pneumoniae.
    J Bacteriol. 1988 Jan;170(1):197-202 PMID: 3275609
  31. DNA mismatch correction.
    Annu Rev Biochem. 1987;56:435-66 PMID: 3304141
  32. Transformation of Schizosaccharomyces pombe by non-homologous, unstable integration of plasmids in the genome.
    Curr Genet. 1986;10(7):503-8 PMID: 3442827
  33. Strains of Schizosaccharomyces pombe with a disrupted swi1 gene still show some mating-type switching.
    Mol Gen Genet. 1987 Dec;210(3):485-9 PMID: 3481026
  34. Protein import into the cell nucleus.
    Annu Rev Cell Biol. 1986;2:367-90 PMID: 3548772
  35. Integration efficiency and genetic recombination in pneumococcal transformation.
    Genetics. 1966 Jan;53(1):207-35 PMID: 4379022
  36. Sequence requirements for nuclear location of simian virus 40 large-T antigen.
    Nature. 1984 Sep 6-11;311(5981):33-8 PMID: 6088992
  37. A short amino acid sequence able to specify nuclear location.
    Cell. 1984 Dec;39(3 Pt 2):499-509 PMID: 6096007
  38. Protein-DNA recognition.
    Annu Rev Biochem. 1984;53:293-321 PMID: 6236744
  39. Construction of a Schizosaccharomyces pombe gene bank in a yeast bacterial shuttle vector and its use to isolate genes by complementation.
    Mol Gen Genet. 1982;187(2):326-9 PMID: 6294466
  40. Identification of the uvrD gene product of Salmonella typhimurium LT2.
    J Bacteriol. 1983 Mar;153(3):1172-9 PMID: 6298177
  41. Rearrangements of the transposable mating-type cassettes of fission yeast.
    EMBO J. 1984 Mar;3(3):603-10 PMID: 6325178
  42. Repair tracts in mismatched DNA heteroduplexes.
    Proc Natl Acad Sci U S A. 1976 Nov;73(11):4135-9 PMID: 1069303
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/20.9.2271
Published
1992-05-11
Pages
2271-8
Language
English
Region
England
NLM ID
0411011
PMCID
PMC312341
Subset
IM
Databases
GENBANK
X61306
External Links
PubMed source DOI: 10.1093/nar/20.9.2271 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2