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PMID: 8849883 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Requirement of mismatch repair genes MSH2 and MSH3 in the RAD1-RAD10 pathway of mitotic recombination in Saccharomyces cerevisiae.

Genetics ·Vol. 142 ·No. 3 ·1996-03-00 ·Pages 727-36

Saparbaev M, Prakash L, Prakash S

Abstract

The RAD1 and RAD10 genes of Saccharomyces cerevisiae are required for nucleotide excision repair and they also act in mitotic recombination. The Rad1-Rad10 complex has a single-stranded DNA endonuclease activity. Here, we show that the mismatch repair genes MSH2 and MSH3 function in mitotic recombination. For both his3 and his4 duplications, and for homologous integration of a linear DNA fragment into the genome, the msh3 delta mutation has an effect on recombination similar to that of the rad1 delta and rad10 delta mutations. The msh2 delta mutation also reduces the rate of recombination of the his3 duplication and lowers the incidence of homologous integration of a linear DNA fragment. Epistasis analyses indicate that MSH2 and MSH3 function in the RAD1-RAD10 recombination pathway, and studies presented here suggest an involvement of the RAD1-RAD10 pathway in reciprocal recombination. The possible roles of Msh2, Msh3, Rad1, and Rad10 proteins in genetic recombination are discussed. Coupling of mismatch binding proteins with the recombinational machinery could be important for ensuring genetic fidelity in the recombination process.

MeSH Terms
Adaptor Proteins, Signal Transducing Adenosine Triphosphatases DNA Repair DNA Repair Enzymes DNA-Binding Proteins/genetics Endonucleases/genetics Fungal Proteins/genetics Gene Expression Regulation, Fungal Mitosis MutL Protein Homolog 1 MutS Homolog 2 Protein MutS Homolog 3 Protein Proteins/genetics Recombination, Genetic Saccharomyces cerevisiae/genetics Saccharomyces cerevisiae Proteins Signal Transduction/genetics Single-Strand Specific DNA and RNA Endonucleases
Chemicals
Adaptor Proteins, Signal Transducing DNA-Binding Proteins Fungal Proteins MLH1 protein, S cerevisiae MSH3 protein, S cerevisiae MutS Homolog 3 Protein Proteins Saccharomyces cerevisiae Proteins Endonucleases RAD1 protein, S cerevisiae RAD10 protein, S cerevisiae Single-Strand Specific DNA and RNA Endonucleases Adenosine Triphosphatases MSH2 protein, S cerevisiae MutL Protein Homolog 1 MutS Homolog 2 Protein DNA Repair Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Saparbaev M
Sealy Center for Molecular Science, University of Texas Medical Branch, Galveston 77555-1061, USA.
Prakash L
Prakash S
References (43)
43 references, click to expand
  1. Inactivation of the mouse Msh2 gene results in mismatch repair deficiency, methylation tolerance, hyperrecombination, and predisposition to cancer.
    Cell. 1995 Jul 28;82(2):321-30 PMID: 7628020
  2. Purification and characterization of the Saccharomyces cerevisiae RAD1/RAD10 endonuclease.
    J Biol Chem. 1993 Dec 15;268(35):26391-9 PMID: 8253764
  3. The role of DNA repair genes in recombination between repeated sequences in yeast.
    Genetics. 1995 Aug;140(4):1199-211 PMID: 7498763
  4. Gene conversion between duplicated genetic elements in yeast.
    Nature. 1981 Jul 23;292(5821):306-11 PMID: 6265790
  5. Nucleotide sequence and functional analysis of the RAD1 gene of Saccharomyces cerevisiae.
    Mol Cell Biol. 1987 Mar;7(3):1012-20 PMID: 3550428
  6. A method for gene disruption that allows repeated use of URA3 selection in the construction of multiply disrupted yeast strains.
    Genetics. 1987 Aug;116(4):541-5 PMID: 3305158
  7. Different types of recombination events are controlled by the RAD1 and RAD52 genes of Saccharomyces cerevisiae.
    Genetics. 1988 Oct;120(2):367-77 PMID: 3058548
  8. New yeast-Escherichia coli shuttle vectors constructed with in vitro mutagenized yeast genes lacking six-base pair restriction sites.
    Gene. 1988 Dec 30;74(2):527-34 PMID: 3073106
  9. The Saccharomyces cerevisiae DNA repair gene RAD25 is required for transcription by RNA polymerase II.
    Genes Dev. 1993 Nov;7(11):2161-71 PMID: 7693549
  10. Destabilization of tracts of simple repetitive DNA in yeast by mutations affecting DNA mismatch repair.
    Nature. 1993 Sep 16;365(6443):274-6 PMID: 8371783
  11. Dual requirement in yeast DNA mismatch repair for MLH1 and PMS1, two homologs of the bacterial mutL gene.
    Mol Cell Biol. 1994 Jan;14(1):407-15 PMID: 8264608
  12. Dual roles of a multiprotein complex from S. cerevisiae in transcription and DNA repair.
    Cell. 1993 Dec 31;75(7):1379-87 PMID: 8269516
  13. DNA repair gene RAD3 of S. cerevisiae is essential for transcription by RNA polymerase II.
    Nature. 1994 Jan 6;367(6458):91-4 PMID: 8107780
  14. DNA repair genes and proteins of Saccharomyces cerevisiae.
    Annu Rev Genet. 1993;27:33-70 PMID: 8122907
  15. Mismatch repair proteins MutS and MutL inhibit RecA-catalyzed strand transfer between diverged DNAs.
    Proc Natl Acad Sci U S A. 1994 Apr 12;91(8):3238-41 PMID: 8159731
  16. Specific association between the human DNA repair proteins XPA and ERCC1.
    Proc Natl Acad Sci U S A. 1994 May 24;91(11):5012-6 PMID: 8197174
  17. Formation of a ternary complex by human XPA, ERCC1, and ERCC4(XPF) excision repair proteins.
    Proc Natl Acad Sci U S A. 1994 May 24;91(11):5017-21 PMID: 8197175
  18. RAD25 is a DNA helicase required for DNA repair and RNA polymerase II transcription.
    Nature. 1994 Jun 16;369(6481):578-81 PMID: 8202161
  19. Interaction between mismatch repair and genetic recombination in Saccharomyces cerevisiae.
    Genetics. 1994 May;137(1):19-39 PMID: 8056309
  20. RNA polymerase transcription factor IIH holoenzyme from yeast.
    J Biol Chem. 1994 Nov 11;269(45):28044-8 PMID: 7961739
  21. Binding of mismatched microsatellite DNA sequences by the human MSH2 protein.
    Science. 1994 Nov 25;266(5189):1403-5 PMID: 7973733
  22. Mutation of a meiosis-specific MutS homolog decreases crossing over but not mismatch correction.
    Cell. 1994 Dec 16;79(6):1069-80 PMID: 8001134
  23. The Saccharomyces cerevisiae Msh2 protein specifically binds to duplex oligonucleotides containing mismatched DNA base pairs and insertions.
    Genes Dev. 1995 Jan 15;9(2):234-47 PMID: 7851796
  24. RAD1 and RAD10, but not other excision repair genes, are required for double-strand break-induced recombination in Saccharomyces cerevisiae.
    Mol Cell Biol. 1995 Apr;15(4):2245-51 PMID: 7891718
  25. Mismatch correction acts as a barrier to homeologous recombination in Saccharomyces cerevisiae.
    Genetics. 1995 Mar;139(3):1175-88 PMID: 7768431
  26. MSH5, a novel MutS homolog, facilitates meiotic reciprocal recombination between homologs in Saccharomyces cerevisiae but not mismatch repair.
    Genes Dev. 1995 Jul 15;9(14):1728-39 PMID: 7622037
  27. Male mice defective in the DNA mismatch repair gene PMS2 exhibit abnormal chromosome synapsis in meiosis.
    Cell. 1995 Jul 28;82(2):309-19 PMID: 7628019
  28. The barrier to recombination between Escherichia coli and Salmonella typhimurium is disrupted in mismatch-repair mutants.
    Nature. 1989 Nov 23;342(6248):396-401 PMID: 2555716
  29. The genetic control of direct-repeat recombination in Saccharomyces: the effect of rad52 and rad1 on mitotic recombination at GAL10, a transcriptionally regulated gene.
    Genetics. 1989 Dec;123(4):725-38 PMID: 2693208
  30. Genetic control of RNA polymerase I-stimulated recombination in yeast.
    Genetics. 1990 Sep;126(1):41-52 PMID: 2227387
  31. Targeting, disruption, replacement, and allele rescue: integrative DNA transformation in yeast.
    Methods Enzymol. 1991;194:281-301 PMID: 2005793
  32. Altering the conserved nucleotide binding motif in the Salmonella typhimurium MutS mismatch repair protein affects both its ATPase and mismatch binding activities.
    EMBO J. 1991 Sep;10(9):2707-15 PMID: 1651234
  33. Control of large chromosomal duplications in Escherichia coli by the mismatch repair system.
    Genetics. 1991 Oct;129(2):327-32 PMID: 1743481
  34. Specific complex formation between proteins encoded by the yeast DNA repair and recombination genes RAD1 and RAD10.
    Proc Natl Acad Sci U S A. 1992 Sep 1;89(17):8273-7 PMID: 1518857
  35. Analysis of a gene conversion gradient at the HIS4 locus in Saccharomyces cerevisiae.
    Genetics. 1992 Sep;132(1):113-23 PMID: 1398048
  36. Genome rearrangement in top3 mutants of Saccharomyces cerevisiae requires a functional RAD1 excision repair gene.
    Mol Cell Biol. 1992 Nov;12(11):4988-93 PMID: 1328869
  37. Removal of nonhomologous DNA ends in double-strand break recombination: the role of the yeast ultraviolet repair gene RAD1.
    Science. 1992 Oct 16;258(5081):480-4 PMID: 1411547
  38. Characterization of insertion mutations in the Saccharomyces cerevisiae MSH1 and MSH2 genes: evidence for separate mitochondrial and nuclear functions.
    Genetics. 1992 Dec;132(4):975-85 PMID: 1334021
  39. The protein sequence and some intron positions are conserved between the switching gene swi10 of Schizosaccharomyces pombe and the human excision repair gene ERCC1.
    Nucleic Acids Res. 1992 Dec 11;20(23):6347-53 PMID: 1475195
  40. Yeast DNA repair and recombination proteins Rad1 and Rad10 constitute a single-stranded-DNA endonuclease.
    Nature. 1993 Apr 29;362(6423):860-2 PMID: 8479526
  41. The yeast gene MSH3 defines a new class of eukaryotic MutS homologues.
    Mol Gen Genet. 1993 May;239(1-2):97-108 PMID: 8510668
  42. Yeast DNA-repair gene RAD14 encodes a zinc metalloprotein with affinity for ultraviolet-damaged DNA.
    Proc Natl Acad Sci U S A. 1993 Jun 15;90(12):5433-7 PMID: 8516285
  43. The Drosophila meiotic recombination gene mei-9 encodes a homologue of the yeast excision repair protein Rad1.
    Genetics. 1995 Oct;141(2):619-27 PMID: 8647398
Article Info
Journal
Genetics
Abbr.
Genetics
ISSN
0016-6731
DOI
10.1093/genetics/142.3.727
Published
1996-03-00
Pages
727-36
Language
English
Region
United States
NLM ID
0374636
PMCID
PMC1207014
Subset
IM
Grants
NCI NIH HHS · CA-35035 · United States
External Links
PubMed source DOI: 10.1093/genetics/142.3.727 PMC Full Text View journal details
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