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PMID: 7498763 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

The role of DNA repair genes in recombination between repeated sequences in yeast.

Genetics ·Vol. 140 ·No. 4 ·1995-08-00 ·Pages 1199-211

Liefshitz B, Parket A, Maya R, Kupiec M

Abstract

The presence of repeated sequences in the genome represents a potential source of karyotypic instability. Genetic control of recombination is thus important to preserve the integrity of the genome. To investigate the genetic control of recombination between repeated sequences, we have created a series of isogenic strains in which we could assess the role of genes involved in DNA repair in two types of recombination: direct repeat recombination and ectopic gene conversion. Naturally occurring (Ty elements) and artificially constructed repeats could be compared in the same cell population. We have found that direct repeat recombination and gene conversion have different genetic requirements. The role of the RAD51, RAD52, RAD54, RAD55, and RAD57 genes, which are involved in recombinational repair, was investigated. Based on the phenotypes of single and double mutants, these genes can be divided into three functional subgroups: one composed of RAD52, a second one composed of RAD51 and RAD54, and a third one that includes the RAD55 and RAD57 genes. Among seven genes involved in excision repair tested, only RAD1 and RAD10 played a role in the types of recombination studied. We did not detect a differential effect of any rad mutation on Ty elements as compared to artificially constructed repeats.

MeSH Terms
Adenosine Triphosphatases Base Sequence DNA Helicases DNA Repair/genetics DNA Repair Enzymes DNA, Fungal/genetics DNA-Binding Proteins/genetics,physiology Endonucleases/genetics,physiology Fungal Proteins/genetics,physiology Gene Conversion/genetics Genes, Fungal Molecular Sequence Data Polymerase Chain Reaction Rad51 Recombinase Rad52 DNA Repair and Recombination Protein Recombination, Genetic Repetitive Sequences, Nucleic Acid Saccharomyces cerevisiae/genetics Saccharomyces cerevisiae Proteins Single-Strand Specific DNA and RNA Endonucleases
Chemicals
DNA, Fungal DNA-Binding Proteins Fungal Proteins RAD52 protein, S cerevisiae RAD55 protein, S cerevisiae Rad52 DNA Repair and Recombination Protein Saccharomyces cerevisiae Proteins RAD51 protein, S cerevisiae Rad51 Recombinase Endonucleases RAD1 protein, S cerevisiae RAD10 protein, S cerevisiae Single-Strand Specific DNA and RNA Endonucleases Adenosine Triphosphatases RAD54 protein, S cerevisiae RAD57 protein, S cerevisiae DNA Helicases DNA Repair Enzymes
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Liefshitz B
Department of Molecular Microbiology and Biotechnology, Tel Aviv University, Ramat Aviv, Israel.
Parket A
Maya R
Kupiec M
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Article Info
Journal
Genetics
Abbr.
Genetics
ISSN
0016-6731
DOI
10.1093/genetics/140.4.1199
Published
1995-08-00
Pages
1199-211
Language
English
Region
United States
NLM ID
0374636
PMCID
PMC1206687
Subset
IM
External Links
PubMed source DOI: 10.1093/genetics/140.4.1199 PMC Full Text View journal details
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