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PMID: 8197174 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Specific association between the human DNA repair proteins XPA and ERCC1.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 91 ·No. 11 ·1994-05-24 ·Pages 5012-6

Li L, Elledge SJ, Peterson CA, Bales ES, Legerski RJ

Abstract

Processing of DNA damage by the nucleotide-excision repair pathway in eukaryotic cells is most likely accomplished by multiprotein complexes. However, the nature of these complexes and the details of the molecular interactions between DNA repair factors are for the most part unknown. Here, we demonstrate both in vivo, using the two-hybrid system, and in vitro, using recombinant proteins, that the human repair factors XPA and ERCC1 specifically interact. In addition, we report an initial determination of the domains in ERCC1 and XPA that mediate this interaction. These results suggest that XPA may play a role in the localization or loading of an incision complex, composed of ERCC1 and possibly other repair factors, onto a damaged site.

MeSH Terms
DNA Damage DNA Repair DNA-Binding Proteins/metabolism Electrophoresis, Polyacrylamide Gel Endonucleases HeLa Cells Humans Protein Binding Proteins/metabolism Xeroderma Pigmentosum Group A Protein
Chemicals
DNA-Binding Proteins Proteins XPA protein, human Xeroderma Pigmentosum Group A Protein ERCC1 protein, human Endonucleases
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Li L
Department of Molecular Genetics, University of Texas M.D. Anderson Cancer Center, Houston 77030.
Elledge S J
Peterson C A
Bales E S
Legerski R J
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.91.11.5012
Published
1994-05-24
Pages
5012-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC43920
Subset
IM
Grants
NIA NIH HHS · AG11085 · United States
NCI NIH HHS · CA52461 · United States
External Links
PubMed source DOI: 10.1073/pnas.91.11.5012 PMC Full Text View journal details
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