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PMID: 8253090 Published · ppublish English Journal Article

Co-correction of the ERCC1, ERCC4 and xeroderma pigmentosum group F DNA repair defects in vitro.

The EMBO journal ·Vol. 12 ·No. 9 ·1993-09-00 ·Pages 3685-92

Biggerstaff M, Szymkowski DE, Wood RD

Abstract

The mammalian ERCC1-encoded polypeptide is required for nucleotide excision repair of damaged DNA and is homologous to Saccharomyces cerevisiae RAD10, which functions in repair and mitotic intrachromosomal recombination. Rodent cells representing repair complementation group 1 have nonfunctional ERCC1. We report that repair of UV-irradiated DNA can be reconstituted by combining rodent group 1 cell extracts with correcting protein from HeLa cells. Background repair was minimized by employing fractionated rodent cell extracts supplemented with human replication proteins RPA and PCNA. Group 1-correcting activity has a native molecular mass of 100 kDa and contains the 33 kDa ERCC1 polypeptide, as well as complementing activities for extracts from rodent group 4 and xeroderma pigmentosum group F (XP-F) cells. Extracts of group 1, group 4 or XP-F cells do not complement one another in vitro, although they complement extracts from other groups. The amount of ERCC1 detectable by immunoblotting is reduced in group 1, group 4 and XP-F extracts. Recombinant ERCC1 from Escherichia coli only weakly corrected the group 1 defect. The data suggest that ERCC1 is part of a functional protein complex with group 4 and XP-F correcting activities. The latter two may be equivalent to one another and analogous to S. cerevisiae RAD1.

MeSH Terms
Amino Acid Sequence Animals Antibodies Base Sequence CHO Cells Cell Line Cricetinae DNA/radiation effects DNA Damage DNA Primers DNA Repair DNA Replication DNA-Binding Proteins/metabolism Endonucleases Escherichia coli Ethylnitrosourea/toxicity Fungal Proteins/metabolism Gene Library Genetic Complementation Test HeLa Cells Humans Immunoblotting Molecular Sequence Data Mutagenesis Peptides/chemical synthesis,immunology Polymerase Chain Reaction Protein Biosynthesis Proteins/metabolism Recombinant Proteins/biosynthesis,metabolism Saccharomyces cerevisiae/metabolism Saccharomyces cerevisiae Proteins Single-Strand Specific DNA and RNA Endonucleases Ultraviolet Rays Xeroderma Pigmentosum/genetics
Chemicals
Antibodies DNA Primers DNA-Binding Proteins Fungal Proteins Peptides Proteins Recombinant Proteins Saccharomyces cerevisiae Proteins xeroderma pigmentosum group F protein DNA ERCC1 protein, human Endonucleases RAD10 protein, S cerevisiae Single-Strand Specific DNA and RNA Endonucleases Ethylnitrosourea
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Biggerstaff M
Imperial Cancer Research Fund, Clare Hall Laboratories, South Mimms, Herts, UK.
Szymkowski D E
Wood R D
References (49)
49 references, click to expand
  1. Molecular characterization of the human excision repair gene ERCC-1: cDNA cloning and amino acid homology with the yeast DNA repair gene RAD10.
    Cell. 1986 Mar 28;44(6):913-23 PMID: 2420469
  2. RAD10, an excision repair gene of Saccharomyces cerevisiae, is involved in the RAD1 pathway of mitotic recombination.
    Mol Cell Biol. 1990 Jun;10(6):2485-91 PMID: 2188090
  3. Determination of molecular weights and frictional ratios of proteins in impure systems by use of gel filtration and density gradient centrifugation. Application to crude preparations of sulfite and hydroxylamine reductases.
    Biochim Biophys Acta. 1966 Feb 7;112(2):346-62 PMID: 5329026
  4. A screening method for isolating DNA repair-deficient mutants of CHO cells.
    Somatic Cell Genet. 1980 May;6(3):391-405 PMID: 7404270
  5. Repair of ultraviolet radiation damage in xeroderma pigmentosum cells belonging to complementation group F.
    Mutat Res. 1981 Feb;80(2):381-8 PMID: 7207491
  6. Genetic diversity of UV-sensitive DNA repair mutants of Chinese hamster ovary cells.
    Proc Natl Acad Sci U S A. 1981 Jun;78(6):3734-7 PMID: 6943579
  7. Repair capability and the cellular age response for killing and mutation induction after UV.
    Mutat Res. 1982 Aug;95(2-3):505-14 PMID: 6889678
  8. Radiation-induced lethality and mutation in a repair-deficient CHO cell line.
    Int J Radiat Biol Relat Stud Phys Chem Med. 1983 Feb;43(2):207-13 PMID: 6600735
  9. Molecular cloning of a human DNA repair gene.
    Nature. 1984 Aug 2-8;310(5976):425-9 PMID: 6462228
  10. Rapid and efficient site-specific mutagenesis without phenotypic selection.
    Proc Natl Acad Sci U S A. 1985 Jan;82(2):488-92 PMID: 3881765
  11. Defective DNA cross-link removal in Chinese hamster cell mutants hypersensitive to bifunctional alkylating agents.
    Cancer Res. 1985 Apr;45(4):1737-43 PMID: 3919945
  12. Genetic complementation between UV-sensitive CHO mutants and xeroderma pigmentosum fibroblasts.
    Mutat Res. 1985 Jun-Jul;150(1-2):423-9 PMID: 4000167
  13. Biological and biochemical consequences of the human ERCC-1 repair gene after transfection into a repair-deficient CHO cell line.
    Mutat Res. 1987 Jan;183(1):69-74 PMID: 3025723
  14. Complementation of the xeroderma pigmentosum DNA repair defect in cell-free extracts.
    Cell. 1988 Apr 8;53(1):97-106 PMID: 3349527
  15. Evolution and mutagenesis of the mammalian excision repair gene ERCC-1.
    Nucleic Acids Res. 1988 Jun 24;16(12):5305-22 PMID: 3290851
  16. Cellular factors required for multiple stages of SV40 DNA replication in vitro.
    EMBO J. 1988 Apr;7(4):1211-8 PMID: 2841119
  17. RAD1, an excision repair gene of Saccharomyces cerevisiae, is also involved in recombination.
    Mol Cell Biol. 1988 Sep;8(9):3619-26 PMID: 3065620
  18. The cloned human DNA excision repair gene ERCC-1 fails to correct xeroderma pigmentosum complementation groups A through I.
    Mutat Res. 1989 Mar;217(2):83-92 PMID: 2918869
  19. Human chromosome 15 confers partial complementation of phenotypes to xeroderma pigmentosum group F cells.
    Am J Hum Genet. 1989 Apr;44(4):474-85 PMID: 2929593
  20. A presumed DNA helicase encoded by ERCC-3 is involved in the human repair disorders xeroderma pigmentosum and Cockayne's syndrome.
    Cell. 1990 Aug 24;62(4):777-91 PMID: 2167179
  21. Analysis of a human DNA excision repair gene involved in group A xeroderma pigmentosum and containing a zinc-finger domain.
    Nature. 1990 Nov 1;348(6296):73-6 PMID: 2234061
  22. Requirement for the replication protein SSB in human DNA excision repair.
    Nature. 1991 Feb 7;349(6309):538-41 PMID: 1992355
  23. Effect of exogenous DNA fragments on human cell extract-mediated DNA repair synthesis.
    Mutat Res. 1991 May;254(3):217-24 PMID: 2052011
  24. Complementation of DNA repair in xeroderma pigmentosum group A cell extracts by a protein with affinity for damaged DNA.
    EMBO J. 1991 Dec;10(12):3913-21 PMID: 1935910
  25. Induction of a mutant phenotype in human repair proficient cells after overexpression of a mutated human DNA repair gene.
    Nucleic Acids Res. 1991 Oct 25;19(20):5633-7 PMID: 1945841
  26. Correction of xeroderma pigmentosum complementation group D mutant cell phenotypes by chromosome and gene transfer: involvement of the human ERCC2 DNA repair gene.
    Proc Natl Acad Sci U S A. 1992 Jan 1;89(1):261-5 PMID: 1729695
  27. Requirement for ERCC-1 and ERCC-3 gene products in DNA excision repair in vitro. Complementation using rodent and human cell extracts.
    J Biol Chem. 1992 Apr 5;267(10):6879-85 PMID: 1551896
  28. Human nucleotide excision nuclease removes thymine dimers from DNA by incising the 22nd phosphodiester bond 5' and the 6th phosphodiester bond 3' to the photodimer.
    Proc Natl Acad Sci U S A. 1992 Apr 15;89(8):3664-8 PMID: 1314396
  29. Proliferating cell nuclear antigen is required for DNA excision repair.
    Cell. 1992 Apr 17;69(2):367-74 PMID: 1348971
  30. Stable and specific association between the yeast recombination and DNA repair proteins RAD1 and RAD10 in vitro.
    Mol Cell Biol. 1992 Jul;12(7):3041-9 PMID: 1620114
  31. Active site of (A)BC excinuclease. I. Evidence for 5' incision by UvrC through a catalytic site involving Asp399, Asp438, Asp466, and His538 residues.
    J Biol Chem. 1992 Sep 5;267(25):17688-92 PMID: 1387639
  32. Specific complex formation between proteins encoded by the yeast DNA repair and recombination genes RAD1 and RAD10.
    Proc Natl Acad Sci U S A. 1992 Sep 1;89(17):8273-7 PMID: 1518857
  33. Oxidative stress and heat shock induce a human gene encoding a protein-tyrosine phosphatase.
    Nature. 1992 Oct 15;359(6396):644-7 PMID: 1406996
  34. Removal of nonhomologous DNA ends in double-strand break recombination: the role of the yeast ultraviolet repair gene RAD1.
    Science. 1992 Oct 16;258(5081):480-4 PMID: 1411547
  35. Identification of the eleventh complementation group of UV-sensitive excision repair-defective rodent mutants.
    Cancer Res. 1992 Dec 1;52(23):6690-1 PMID: 1423315
  36. ERCC6, a member of a subfamily of putative helicases, is involved in Cockayne's syndrome and preferential repair of active genes.
    Cell. 1992 Dec 11;71(6):939-53 PMID: 1339317
  37. A case of xeroderma pigmentosum complementation group F with neurological abnormalities.
    Br J Dermatol. 1993 Jan;128(1):91-4 PMID: 8427828
  38. Purification and characterization of a human protein that binds to damaged DNA.
    Biochemistry. 1993 Feb 16;32(6):1657-66 PMID: 8431446
  39. Mechanism of action of the Escherichia coli UvrABC nuclease: clues to the damage recognition problem.
    Bioessays. 1993 Jan;15(1):51-9 PMID: 8466476
  40. Yeast DNA repair and recombination proteins Rad1 and Rad10 constitute a single-stranded-DNA endonuclease.
    Nature. 1993 Apr 29;362(6423):860-2 PMID: 8479526
  41. Complementation of the DNA repair defect in xeroderma pigmentosum group G cells by a human cDNA related to yeast RAD2.
    Nature. 1993 May 13;363(6425):182-5 PMID: 8483504
  42. Identical defects in DNA repair in xeroderma pigmentosum group G and rodent ERCC group 5.
    Nature. 1993 May 13;363(6425):185-8 PMID: 8483505
  43. Genetic analysis of nucleotide excision repair in mammalian cells.
    Semin Cancer Biol. 1993 Apr;4(2):105-17 PMID: 8513146
  44. Evidence for a repair enzyme complex involving ERCC1 and complementing activities of ERCC4, ERCC11 and xeroderma pigmentosum group F.
    EMBO J. 1993 Sep;12(9):3693-701 PMID: 8253091
  45. Summary of complementation groups of UV-sensitive CHO cell mutants isolated by large-scale screening.
    Mutagenesis. 1989 Sep;4(5):349-54 PMID: 2687628
  46. Yeast intrachromosomal recombination: long gene conversion tracts are preferentially associated with reciprocal exchange and require the RAD1 and RAD3 gene products.
    Genetics. 1989 Dec;123(4):683-94 PMID: 2558957
  47. The genetic control of direct-repeat recombination in Saccharomyces: the effect of rad52 and rad1 on mitotic recombination at GAL10, a transcriptionally regulated gene.
    Genetics. 1989 Dec;123(4):725-38 PMID: 2693208
  48. ERCC2: cDNA cloning and molecular characterization of a human nucleotide excision repair gene with high homology to yeast RAD3.
    EMBO J. 1990 May;9(5):1437-47 PMID: 2184031
  49. Domainal evolution of a prokaryotic DNA repair protein and its relationship to active-transport proteins.
    Nature. 1986 Oct 2-8;323(6087):451-3 PMID: 3762695
Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1993-09-00
Pages
3685-92
Language
English
Region
England
NLM ID
8208664
PMCID
PMC413645
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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