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PMID: 3290851 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Evolution and mutagenesis of the mammalian excision repair gene ERCC-1.

Nucleic acids research ·Vol. 16 ·No. 12 ·1988-06-24 ·Pages 5305-22

van Duin M, van den Tol J, Warmerdam P, Odijk H, Meijer D, Westerveld A, Bootsma D, Hoeijmakers JH

Abstract

The human DNA excision repair protein ERCC-1 exhibits homology to the yeast RAD10 repair protein and its longer C-terminus displays similarity to parts of the E. coli repair proteins uvrA and uvrC. To study the evolution of this 'mosaic' ERCC-1 gene we have isolated the mouse homologue. Mouse ERCC-1 harbors the same pattern of homology with RAD10 and has a comparable C-terminal extension as its human equivalent. Mutation studies show that the strongly conserved C-terminus is essential in contrast to the less conserved N-terminus which is even dispensible. The mouse ERCC-1 amino acid sequence is compatible with a previously postulated nuclear location signal and DNA-binding domain. The ERCC-1 promoter harbors a region which is highly conserved in mouse and man. Since the ERCC-1 promoter is devoid of all classical promoter elements this region may be responsible for the low constitutive level of expression in all mouse tissues and stages of embryogenesis examined.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Biological Evolution Cell Line Cloning, Molecular DNA Repair DNA Replication Escherichia coli/genetics Genes Humans Mice Molecular Sequence Data Nucleic Acid Hybridization Plasmids Proteins/genetics Saccharomyces cerevisiae/genetics Sequence Homology, Nucleic Acid
Chemicals
Proteins
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
van Duin M
Department of Cell Biology and Genetics, Erasmus University, Rotterdam, The Netherlands.
van den Tol J
Warmerdam P
Odijk H
Meijer D
Westerveld A
Bootsma D
Hoeijmakers J H
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/16.12.5305
Published
1988-06-24
Pages
5305-22
Language
English
Region
England
NLM ID
0411011
PMCID
PMC336769
Subset
IM
Databases
GENBANK
X07413, X07414
External Links
PubMed source DOI: 10.1093/nar/16.12.5305 PMC Full Text View journal details
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