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PMID: 3684592 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Genomic characterization of the human DNA excision repair gene ERCC-1.

Nucleic acids research ·Vol. 15 ·No. 22 ·1987-11-25 ·Pages 9195-213

van Duin M, Koken MH, van den Tol J, ten Dijke P, Odijk H, Westerveld A, Bootsma D, Hoeijmakers JH

Abstract

In this report the genomic characterization of the human excision repair gene ERCC-1 is presented. The gene consists of 10 exons spread over approximately 15 kb. By means of a transfection assay the ERCC-1 promoter was confined to a region of +/- 170 bp upstream of the transcriptional start site. Classical promoter elements like CAAT, TATA and GC-boxes are absent from this region. Furthermore, ERCC-1 transcription is not UV-inducible. A possible explanation is provided for the previously reported alternative splicing of exon VIII. Analysis of ERCC-1 cDNA clones revealed the occurrence of differential polyadenylation which gives ERCC-1 transcripts of 3.4 and 3.8 kb in addition to the major 1.1 kb mRNA. Apparent evolutionary conservation of differential polyadenylation of ERCC-1 transcripts suggests a possible role for this mode of RNA processing in the ERCC-1 repair function.

MeSH Terms
Animals Base Sequence Cell Line DNA/isolation & purification DNA Repair Genes HeLa Cells/metabolism Humans Molecular Sequence Data Plasmids Promoter Regions, Genetic Transfection Ultraviolet Rays
Chemicals
DNA
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
van Duin M
Department of Cell Biology and Genetics, Erasmus University, Rotterdam, The Netherlands.
Koken M H
van den Tol J
ten Dijke P
Odijk H
Westerveld A
Bootsma D
Hoeijmakers J H
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/15.22.9195
Published
1987-11-25
Pages
9195-213
Language
English
Region
England
NLM ID
0411011
PMCID
PMC306462
Subset
IM
Databases
GENBANK
X06581, X06582, X07415
External Links
PubMed source DOI: 10.1093/nar/15.22.9195 PMC Full Text View journal details
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