Home LiteratureArticle Details
PMID: 3008106 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

The organization of the human HPRT gene.

Nucleic acids research ·Vol. 14 ·No. 7 ·1986-04-11 ·Pages 3103-18

Kim SH, Moores JC, David D, Respess JG, Jolly DJ, Friedmann T

Abstract

The organization of the X-linked gene for human hypoxanthine phosphoribosyltransferase (HPRT, EC 2.4.2.8.) has been determined by a combination of restriction endonuclease mapping, heteroduplex analysis and DNA sequence analysis of overlapping genomic clones. The entire gene is 42 kilobases in length and split into 9 exons. The sizes of the 7 internal exons and the exon-intron boundaries are identical to those of mouse HPRT gene. The 5' end of the gene lacks the prototypical 5' transcriptional regulatory sequence elements but contains extremely GC-rich sequences and five GC hexanucleotide motifs (5'-GGCGGG-3'). These structural features are very similar to those found in the mouse HPRT gene and to some of the regulatory signals common to a class of constitutively expressed "housekeeping" genes. Several transcriptional start sites have been identified by nuclease protection studies. Extensive sequence homology between the mouse and human genes is found in the 3' non-coding portion of the gene.

MeSH Terms
Animals Base Sequence Cell Line Cricetinae DNA/analysis DNA Restriction Enzymes/metabolism Humans Hypoxanthine Phosphoribosyltransferase/genetics Isoelectric Focusing Mice Nucleic Acid Conformation Ribonucleases/metabolism Transfection
Chemicals
DNA Hypoxanthine Phosphoribosyltransferase Ribonucleases DNA Restriction Enzymes
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Kim S H
Moores J C
David D
Respess J G
Jolly D J
Friedmann T
References (40)
40 references, click to expand
  1. Heterogeneity at the 5' termini of mouse dihydrofolate reductase mRNAs. Evidence for multiple promoter regions.
    J Biol Chem. 1985 Feb 25;260(4):2307-14 PMID: 2982814
  2. Multiple specific contacts between a mammalian transcription factor and its cognate promoters.
    Nature. 1984 Nov 29-Dec 5;312(5993):409-13 PMID: 6095100
  3. Transcriptional regulation of mouse dihydrofolate reductase in the cell cycle.
    J Biol Chem. 1985 Jun 25;260(12):7675-80 PMID: 2987264
  4. Faithful cell-cycle regulation of a recombinant mouse histone H4 gene is controlled by sequences in the 3'-terminal part of the gene.
    Proc Natl Acad Sci U S A. 1985 Jul;82(13):4389-93 PMID: 3925455
  5. Adenosine deaminase: characterization and expression of a gene with a remarkable promoter.
    EMBO J. 1985 Feb;4(2):437-43 PMID: 3839456
  6. Characterization and sequence of the promoter region of the human epidermal growth factor receptor gene.
    Proc Natl Acad Sci U S A. 1985 Aug;82(15):4920-4 PMID: 2991899
  7. Control of eukaryotic messenger RNA synthesis by sequence-specific DNA-binding proteins.
    Nature. 1985 Aug 29-Sep 4;316(6031):774-8 PMID: 4041012
  8. The use of drug-resistant markers to study the hybridization of mouse fibroblasts.
    Exp Cell Res. 1966 Jan;41(1):190-6 PMID: 4952025
  9. Enzyme defect associated with a sex-linked human neurological disorder and excessive purine synthesis.
    Science. 1967 Mar 31;155(3770):1682-4 PMID: 6020292
  10. A specific enzyme defect in gout associated with overproduction of uric acid.
    Proc Natl Acad Sci U S A. 1967 Jun;57(6):1735-9 PMID: 4291947
  11. Human lymphoblastoid lines from lymph node and spleen.
    Cancer. 1968 Sep;22(3):517-24 PMID: 5673231
  12. Hypoxanthine-guanine phosphoribosyltransferase deficiency in gout.
    Ann Intern Med. 1969 Jan;70(1):155-206 PMID: 4884382
  13. A new technique for the assay of infectivity of human adenovirus 5 DNA.
    Virology. 1973 Apr;52(2):456-67 PMID: 4705382
  14. Improved estimation of secondary structure in ribonucleic acids.
    Nat New Biol. 1973 Nov 14;246(150):40-1 PMID: 4519026
  15. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  16. Human and mouse hypoxanthine-guanine phosphoribosyltransferase: dimers and tetramers.
    Science. 1979 Jan 12;203(4376):174-6 PMID: 569362
  17. The isolation and characterization of linked delta- and beta-globin genes from a cloned library of human DNA.
    Cell. 1978 Dec;15(4):1157-74 PMID: 728996
  18. Sequencing end-labeled DNA with base-specific chemical cleavages.
    Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
  19. Molecular cloning and characterization of the human beta-like globin gene cluster.
    Cell. 1980 Apr;19(4):959-72 PMID: 6155216
  20. Organization and expression of eucaryotic split genes coding for proteins.
    Annu Rev Biochem. 1981;50:349-83 PMID: 6791577
  21. Pattern recognition in nucleic acid sequences. I. A general method for finding local homologies and symmetries.
    Nucleic Acids Res. 1982 Jan 11;10(1):247-63 PMID: 6801626
  22. Cloned cDNA sequences of the hypoxanthine/guanine phosphoribosyltransferase gene from a mouse neuroblastoma cell line found to have amplified genomic sequences.
    Proc Natl Acad Sci U S A. 1982 Mar;79(6):1950-4 PMID: 6952245
  23. An interactive graphics program for comparing and aligning nucleic acid and amino acid sequences.
    Nucleic Acids Res. 1982 May 11;10(9):2951-61 PMID: 7099970
  24. Isolation of a genomic clone partially encoding human hypoxanthine phosphoribosyltransferase.
    Proc Natl Acad Sci U S A. 1982 Aug;79(16):5038-41 PMID: 6956912
  25. The sequences of an expressed rat alpha-tubulin gene and a pseudogene with an inserted repetitive element.
    Nature. 1982 Nov 25;300(5890):330-5 PMID: 6927856
  26. The construction of cosmid libraries which can be used to transform eukaryotic cells.
    Nucleic Acids Res. 1982 Nov 11;10(21):6715-32 PMID: 6294612
  27. Hypoxanthine-guanine phosphoribosyltransferase genes of mouse and Chinese hamster: construction and sequence analysis of cDNA recombinants.
    Nucleic Acids Res. 1982 Nov 11;10(21):6763-75 PMID: 6294614
  28. Recombination during gene transfer into mouse cells can restore the function of deleted genes.
    Science. 1983 Jan 14;219(4581):174-6 PMID: 6294829
  29. Isolation and characterization of a full-length expressible cDNA for human hypoxanthine phosphoribosyl transferase.
    Proc Natl Acad Sci U S A. 1983 Jan;80(2):477-81 PMID: 6300847
  30. Homologous recombination in mammalian cells mediates formation of a functional gene from two overlapping gene fragments.
    Proc Natl Acad Sci U S A. 1983 Apr;80(7):2002-6 PMID: 6132383
  31. Transcription in vivo from SV40 early promoter deletion mutants without repression by large T antigen.
    J Mol Appl Genet. 1983;2(1):127-35 PMID: 6842119
  32. The promoter-specific transcription factor Sp1 binds to upstream sequences in the SV40 early promoter.
    Cell. 1983 Nov;35(1):79-87 PMID: 6313230
  33. Growth-dependent expression of dihydrofolate reductase mRNA from modular cDNA genes.
    Mol Cell Biol. 1983 Sep;3(9):1598-608 PMID: 6138708
  34. Lambda replacement vectors carrying polylinker sequences.
    J Mol Biol. 1983 Nov 15;170(4):827-42 PMID: 6315951
  35. The functional human dihydrofolate reductase gene.
    J Biol Chem. 1984 Mar 25;259(6):3933-43 PMID: 6323448
  36. Structure, expression, and mutation of the hypoxanthine phosphoribosyltransferase gene.
    Proc Natl Acad Sci U S A. 1984 Apr;81(7):2147-51 PMID: 6326107
  37. Homologous recombination between plasmids in mammalian cells can be enhanced by treatment of input DNA.
    Proc Natl Acad Sci U S A. 1984 May;81(10):3153-7 PMID: 6328502
  38. HMG CoA reductase: a negatively regulated gene with unusual promoter and 5' untranslated regions.
    Cell. 1984 Aug;38(1):275-85 PMID: 6088070
  39. Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter.
    Nucleic Acids Res. 1984 Sep 25;12(18):7035-56 PMID: 6091052
  40. Sequence of the promoter region of the gene for human X-linked 3-phosphoglycerate kinase.
    Gene. 1984 Dec;32(3):409-17 PMID: 6099325
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/14.7.3103
Published
1986-04-11
Pages
3103-18
Language
English
Region
England
NLM ID
0411011
PMCID
PMC339724
Subset
IM
Grants
NIADDK NIH HHS · 5T32 AM07318 · United States
NIGMS NIH HHS · GM28223 · United States
NICHD NIH HHS · HD20034 · United States
External Links
PubMed source DOI: 10.1093/nar/14.7.3103 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2