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PMID: 6209716 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

Intron splicing: a conserved internal signal in introns of animal pre-mRNAs.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 81 ·No. 23 ·1984-12-00 ·Pages 7417-20

Keller EB, Noon WA

Abstract

Splicing of introns of yeast pre-mRNAs requires an internal conserved sequence T-A-C-T-A-A-C that is located 20-55 nucleotides from the 3' intron boundary. Sequences differing only in certain positions from this yeast signal have now been identified in the corresponding internal region of pre-mRNA introns of a variety of animal genes. A computer program that searches for homologues to a consensus structure and calculates the accuracy of match of each homologue is used to locate these sequences. We list here the signals found by this search in introns of sea urchin, mouse, rat, and human genes and give the consensus for each species. We also give the consensus found for Drosophila and chicken and duck signals. We then discuss the accumulating evidence that these internal signals are required for splicing in animals. It is also noted that a single-stranded region of small nuclear RNA U2 contains sequences complementary both to the proposed mammalian internal signal and to the neighboring CT-A-G at the 3' intron boundary. A role for U2 ribonucleoprotein in intron splicing is thus suggested.

MeSH Terms
Animals Base Sequence Chickens Drosophila/genetics Ducks Genes Mice Neurospora crassa/genetics Nucleic Acid Precursors/genetics RNA/genetics RNA Precursors RNA Splicing RNA, Messenger/genetics RNA, Small Nuclear Rats Species Specificity
Chemicals
Nucleic Acid Precursors RNA Precursors RNA, Messenger RNA, Small Nuclear RNA
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Keller E B
Noon W A
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45 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.81.23.7417
Published
1984-12-00
Pages
7417-20
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC392157
Subset
IM
Grants
NIGMS NIH HHS · GM10791 · United States
External Links
PubMed source DOI: 10.1073/pnas.81.23.7417 PMC Full Text View journal details
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