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PMID: 6310494 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

The genes coding for histone H3 and H4 in Neurospora crassa are unique and contain intervening sequences.

Nucleic acids research ·Vol. 11 ·No. 16 ·1983-08-25 ·Pages 5347-60

Woudt LP, Pastink A, Kempers-Veenstra AE, Jansen AE, Mager WH, Planta RJ

Abstract

Sequences coding for histone H3 and H4 of Neurospora crassa could be identified in genomic digests with the use of the corresponding genes from sea urchin and X. laevis as hybridization probes. A 2.6 kb HindIII-generated N. crassa DNA fragment, showing homology with the heterologous histone H3-gene probes was cloned in a charon 21A vector. Using DNA from this clone as a homologous hybridization probe a 6.9 kb SalI-generated DNA fragment was isolated which in addition to the histone H3-gene also contains the gene coding for histone H4. Several lines of evidence demonstrate the presence of only a single histone H3- as well as a single histone H4-gene in N. crassa. The two genes are physically linked on the genome. DNA sequencing of the N. crassa histone H3- and H4-genes confirmed their identity and, in addition, revealed the presence of one short intron (67 bp) within the coding sequence of the H3-gene and even two introns (68 and 69 bp) within the H4-gene. The amino acid sequences of the N. crassa histones H3 and H4, as deduced from the DNA sequences, and those of the corresponding yeast histones differ only at a few positions. Much larger sequence differences, however, are observed at the DNA level, reflecting a diverging codon usage in the two lower eukaryotes.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Cloning, Molecular DNA Restriction Enzymes DNA, Recombinant/metabolism Genes Histones/genetics Nucleic Acid Hybridization Plasmids Saccharomyces cerevisiae/genetics Sea Urchins Species Specificity Xenopus
Chemicals
DNA, Recombinant Histones DNA Restriction Enzymes
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Woudt L P
Pastink A
Kempers-Veenstra A E
Jansen A E
Mager W H
Planta R J
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31 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/11.16.5347
Published
1983-08-25
Pages
5347-60
Language
English
Region
England
NLM ID
0411011
PMCID
PMC326282
Subset
IM
Databases
GENBANK
K03154, X01611, X01612
External Links
PubMed source DOI: 10.1093/nar/11.16.5347 PMC Full Text View journal details
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