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PMID: 3912171 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Nucleotide sequence of the RAD10 gene of Saccharomyces cerevisiae.

The EMBO journal ·Vol. 4 ·No. 13A ·1985-12-16 ·Pages 3549-52

Reynolds P, Prakash L, Dumais D, Perozzi G, Prakash S

Abstract

The RAD10 gene is one of several genes in Saccharomyces cerevisiae required for incision of u.v.-irradiated or cross-linked DNA. We have determined the nucleotide sequence of the RAD10 gene and its flanking regions. The RAD10 nucleotide sequence presented here differs significantly from that recently reported. The RAD10 protein predicted from the nucleotide sequence contains 210 amino acids with a calculated mol. wt. of 24 310. The middle portion of the RAD10 protein, which is highly basic and also contains eight of the total of 10 tyrosine residues present in the protein, may be involved in DNA binding by ionic interactions and tyrosine intercalation between the bases of DNA. A genomic deletion of the entire RAD10 gene does not affect viability; however, the rad10 deletion mutant is highly u.v. sensitive.

MeSH Terms
Amino Acid Sequence Base Sequence Chromosome Deletion Codon DNA Repair DNA, Fungal/genetics DNA-Binding Proteins/genetics Genes Genes, Fungal Genetic Linkage Saccharomyces cerevisiae/genetics
Chemicals
Codon DNA, Fungal DNA-Binding Proteins
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Reynolds P
Prakash L
Dumais D
Perozzi G
Prakash S
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37 references, click to expand
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1985-12-16
Pages
3549-52
Language
English
Region
England
NLM ID
8208664
PMCID
PMC554695
Subset
IM
Grants
NCI NIH HHS · CA35035 · United States
Databases
GENBANK
X05225
External Links
PubMed source PMC Full Text View journal details
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