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PMID: 2184031 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

ERCC2: cDNA cloning and molecular characterization of a human nucleotide excision repair gene with high homology to yeast RAD3.

The EMBO journal ·Vol. 9 ·No. 5 ·1990-05-00 ·Pages 1437-47

Weber CA, Salazar EP, Stewart SA, Thompson LH

Abstract

Human ERCC2 genomic clones give efficient, stable correction of the nucleotide excision repair defect in UV5 Chinese hamster ovary cells. One clone having a breakpoint just 5' of classical promoter elements corrects only transiently, implicating further flanking sequences in stable gene expression. The nucleotide sequences of a cDNA clone and genomic flanking regions were determined. The ERCC2 translated amino acid sequence has 52% identity (73% homology) with the yeast nucleotide excision repair protein RAD3. RAD3 is essential for cell viability and encodes a protein that is a single-stranded DNA dependent ATPase and an ATP dependent helicase. The similarity of ERCC2 and RAD3 suggests a role for ERCC2 in both cell viability and DNA repair and provides the first insight into the biochemical function of a mammalian nucleotide excision repair gene.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Cell Survival/genetics Cloning, Molecular DNA Repair/genetics Gene Library Genes, Fungal/genetics Genetic Complementation Test Humans Introns/genetics Molecular Sequence Data Regulatory Sequences, Nucleic Acid Restriction Mapping Saccharomyces cerevisiae/genetics Sequence Homology, Nucleic Acid Transcription, Genetic/genetics Transformation, Genetic Ultraviolet Rays
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Weber C A
Biomedical Sciences Division, Lawrence Livermore National Laboratory, Livermore, CA 94550.
Salazar E P
Stewart S A
Thompson L H
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1990-05-00
Pages
1437-47
Language
English
Region
England
NLM ID
8208664
PMCID
PMC551832
Subset
IM
Databases
GENBANK
X52221, X52222, X52470
External Links
PubMed source PMC Full Text View journal details
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