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PMID: 2827162 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

RAD3 protein of Saccharomyces cerevisiae is a DNA helicase.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 84 ·No. 24 ·1987-12-00 ·Pages 8951-5

Sung P, Prakash L, Matson SW, Prakash S

Abstract

The Saccharomyces cerevisiae RAD3 gene, which is required for cell viability and excision repair of damaged DNA, encodes an 89-kDa protein that has a single-stranded DNA-dependent ATPase activity. We now show that the RAD3 protein also possesses a helicase activity that unwinds duplex regions in DNA substrates constructed by annealing DNA fragments of 71-851 nucleotides to circular, single-stranded M13 DNA. The DNA helicase activity is dependent on the hydrolysis of ATP, has a pH optimum of approximately 5.6, and is inhibited by antibodies raised against a truncated RAD3 protein produced in Escherichia coli. The RAD3 helicase translocates along single-stranded DNA in the 5'----3' direction. The direction of RAD3 helicase movement is consistent with the possibility that it unwinds DNA duplexes in advance of the replication fork during DNA replication.

MeSH Terms
Antigen-Antibody Reactions DNA Helicases/isolation & purification,physiology DNA Repair DNA Replication Fungal Proteins/isolation & purification,physiology Saccharomyces cerevisiae/physiology
Chemicals
Fungal Proteins DNA Helicases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Sung P
Department of Biology, University of Rochester, NY 14627.
Prakash L
Matson S W
Prakash S
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34 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.84.24.8951
Published
1987-12-00
Pages
8951-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC299669
Subset
IM
Grants
NCI NIH HHS · CA-35035 · United States
NIGMS NIH HHS · GM19261 · United States
NIGMS NIH HHS · GM33476 · United States
External Links
PubMed source DOI: 10.1073/pnas.84.24.8951 PMC Full Text View journal details
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