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PMID: 2017172 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

Evi-1, a murine zinc finger proto-oncogene, encodes a sequence-specific DNA-binding protein.

Molecular and cellular biology ·Vol. 11 ·No. 5 ·1991-05-00 ·Pages 2665-74

Perkins AS, Fishel R, Jenkins NA, Copeland NG

Abstract

Evi-1 was originally identified as a common site of viral integration in murine myeloid tumors. Evi-1 encodes a 120-kDa polypeptide containing 10 zinc finger motifs located in two domains 380 amino acids apart and an acidic domain located carboxy terminal to the second set of zinc fingers. These features suggest that Evi-1 is a site-specific DNA-binding protein involved in the regulation of RNA transcription. We have purified Evi-1 protein from E. coli and have employed a gel shift-polymerase chain reaction method using random oligonucleotides to identify a high-affinity binding site for Evi-1. The consensus sequence for this binding site is TGACAAGATAA. Evi-1 protein specifically protects this motif from DNase I digestion. By searching the nucleotide sequence data bases, we have found this binding site both in sequences 5' to genes in putative or known regulatory regions and within intron sequences.

Related Genes
Evi-1
MeSH Terms
Animals Base Sequence Binding Sites Blotting, Western Cloning, Molecular DNA-Binding Proteins/genetics,isolation & purification Escherichia coli/genetics MDS1 and EVI1 Complex Locus Protein Mice Molecular Sequence Data Molecular Weight Oligonucleotide Probes Polymerase Chain Reaction/methods Proto-Oncogenes Recombinant Fusion Proteins/isolation & purification Sequence Homology, Nucleic Acid Transcription Factors Zinc Fingers/genetics
Chemicals
DNA-Binding Proteins MDS1 and EVI1 Complex Locus Protein Mecom protein, mouse Oligonucleotide Probes Recombinant Fusion Proteins Transcription Factors
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Perkins A S
Mammalian Genetics Laboratory, NCI-Frederick Cancer Research and Development Center, Maryland 21702.
Fishel R
Jenkins N A
Copeland N G
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.11.5.2665-2674.1991
Published
1991-05-00
Pages
2665-74
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC360036
Subset
IM
Grants
NCI NIH HHS · N01-CO-74101 · United States
External Links
PubMed source DOI: 10.1128/mcb.11.5.2665-2674.1991 PMC Full Text View journal details
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